Results 71 to 80 of about 90,326,780 (286)

A light‐triggered Time‐Resolved X‐ray Solution Scattering (TR‐XSS) workflow with application to protein conformational dynamics

FEBS Open Bio, EarlyView.
Time‐resolved X‐ray solution scattering captures how proteins change shape in real time under near‐native conditions. This article presents a practical workflow for light‐triggered TR‐XSS experiments, from data collection to structural refinement. Using a calcium‐transporting membrane protein as an example, the approach can be broadly applied to study ...
Fatemeh Sabzian‐Molaei, Fredrik Orädd, Konstantinos Magkakis, Magnus Andersson   +3 more
wiley   +1 more source

Single‐molecule DNA flow‐stretch assays for high‐throughput DNA–protein interaction studies

FEBS Open Bio, EarlyView.
We describe an optimised single‐molecule DNA flow‐stretch assay that visualises DNA–protein interactions in real time. Linear DNA fragments are tethered to a surface and stretched by buffer flow for fluorescence imaging. Using λ and φX174 DNA, this protocol enhances reproducibility and accessibility, providing a versatile approach for studying diverse ...
Ayush Kumar Ganguli, Mohammad Nour Alsamsam, Ugnė Bagdonaitė, Van Truc Vu, Chun‐Jen Huang, Polina Kuzhir, Mindaugas Zaremba, Aurimas Kopūstas, Marijonas Tutkus   +8 more
wiley   +1 more source

Developing predictions for pion fragmentation functions

European Physical Journal C: Particles and Fields
Exploiting crossing symmetry, the hadron scale pion valence quark distribution function is used to predict the kindred elementary valence quark fragmentation function (FF).
H.-Y. Xing, Z.-Q. Yao, B.-L. Li, D. Binosi, Z.-F. Cui, C. D. Roberts   +5 more
doaj   +1 more source

λϕ 4 theory — Part II. the broken phase beyond NNNN(NNNN)LO

Journal of High Energy Physics, 2019
We extend the study of the two-dimensional euclidean ϕ 4 theory initiated in ref. [1] to the ℤ2 broken phase. In particular, we compute in perturbation theory up to N4LO in the quartic coupling the vacuum energy, the vacuum expectation value of ϕ and the
Marco Serone, Gabriele Spada, Giovanni Villadoro   +2 more
doaj   +1 more source

Analysing the significance of small conformational changes and low occupancy states in serial crystallographic data

FEBS Open Bio, EarlyView.
This protocol paper outlines methods to establish the success of a time‐resolved serial crystallographic experiment, by means of statistical analysis of timepoint data in reciprocal space and models in real space. We show how to amplify the signal from excited states to visualise structural changes in successful experiments.
Jake Hill, Yelyzaveta Pulnova, Elke De Zitter, Helen M. Ginn, Briony A. Yorke   +4 more
wiley   +1 more source

λϕ 4 theory — Part I. The symmetric phase beyond NNNNNNNNLO

Journal of High Energy Physics, 2018
Perturbation theory of a large class of scalar field theories in d < 4 can be shown to be Borel resummable using arguments based on Lefschetz thimbles. As an example we study in detail the λϕ 4 theory in two dimensions in the Z 2 symmetric phase.
Marco Serone, Gabriele Spada, Giovanni Villadoro   +2 more
doaj   +1 more source

Coordination of CO to low-valent phosphorus centres and other related P–C bonding situations. A theoretical case study

Chemical Science, 2013
The multi-faceted bonding of CO in molecular phosphorus compounds is described using calculated P–C bond strengths as a criterion. Full compliance matrices at coupled cluster level of HPCO (1a), singlet oxaphosphirane-3-ylidene HP(η2-CO)), the dimer (HPCO)2 as well as PCH, HPCH2 and H2P–CH3 were calculated to obtain quantifiable data and enable ...
Gerd von Frantzius, Arturo Espinosa Ferao, Rainer Streubel   +2 more
openaire   +1 more source

Sudden anaerobization in Amphibacillus xylanus increases intracellular labile ferrous iron and inhibits cell growth

FEBS Open Bio, EarlyView.
Abruptly changing from aerobic to anaerobic conditions (sudden anaerobization) induced growth inhibition and a significant increase in intracellular labile ferrous iron in the aerotolerant anaerobe Amphibacillus xylanus. We found that free flavins mediate efficient electron transfer from NADH to ferric iron under anaerobic conditions, suggesting that ...
Shinya Kimata, Yoichi Sakai, Keisuke Tanaka, Etsuro Yoshimura, Ken Kitano, Morio Ishikawa, Tadahiro Nakamoto, Tsutomu Takayama, Hirofumi Inoue, Kaho Nomura, Tomonori Suzuki, Masataka Uchino, Akira Abe, Youichi Niimura   +13 more
wiley   +1 more source

Neumann-Rosochatius system for strings in ABJ model

Journal of High Energy Physics, 2019
Neumann-Rosochatius system is a well known one dimensional integrable sys- tem. We study the rotating and pulsating string in AdS4 × ℂℙ3 with a B NS holonomy turned on over ℂℙ1 ⊂ ℂℙ3, the so called Aharony-Bergman-Jafferis (ABJ) background.
Adrita Chakraborty, Kamal L. Panigrahi
doaj   +1 more source

RoundMi: A quantitative method to analyze mitochondrial morphology in mitotic cells

FEBS Open Bio, EarlyView.
RoundMi is a workflow for rapid analysis of mitochondrial morphology in mitotic cells. By combining adaptive preprocessing with automated segmentation and quantification, it enables accurate measurements from single focal plane images, reducing acquisition time and computational demands while remaining compatible with high‐throughput fixed and live ...
Elmira Parvindokht Bararpour, Christine Volmert, Valentina Piano   +2 more
wiley   +1 more source