Results 81 to 90 of about 786,883 (190)

Phase retention control in preparative centrifugal partition chromatography

Journal of Chromatography A
Centrifugal Partition Chromatography (CPC) is a separation technique based on Liquid-Liquid Chromatography (LLC) using two immiscible liquids as mobile and stationary phases. The latter is immobilized in separation chambers by the application of a centrifugal field.
Jan Hohlmann, Antonia Göring, Fabian Rhein, Felix Buthmann, Gerhard Schembecker   +4 more
openaire   +2 more sources

Multi‐Omics Integration Reveals Metabolic Reprogramming and EMT‐Driven Metastasis in Triple‐Negative Breast Cancer

Cancer Science, EarlyView.
Integrated transcriptomic, metabolomic, and spatial proteomic profiling of TNBC tumors identifies a metastatic‐associated molecular landscape characterized by EMT activation, metabolic reprogramming, and tumor microenvironment (TME) remodeling. Multi‐omics integration reveals coordinated alterations in EMT signatures, amino acid metabolism, immune ...
Yuheng Pang, Yuefeng Shang, Wenjing Wang, Tong Liu, Xiaolong Zou   +4 more
wiley   +1 more source

Biocatalytic synthesis of S‐adenosyl‐l‐methionine analogues for allosteric activation of cystathionine‐β‐synthase

The FEBS Journal, EarlyView.
Cystathionine‐β‐synthase (CBS) helps remove harmful homocysteine and supports the production of protective sulfur‐containing molecules. Using its natural activator, S‐adenosyl‐l‐methionine, as a template, we designed and tested 15 analogues. Three activated and stabilized the enzyme, including the newly identified S‐2‐amino‐adenosyl‐l‐methionine. These
Dalibor Nakládal, Žaneta Zajacová, Rick Oerlemans, Jana Havlikova, Gabriel Zorkócy, Lukáš Kerti, Katarína Sujová, Adrian Lengyel, Zoë van der Gun, Christina Yoseif, Adrianus Cornelis van der Graaf, Guido Krenning, Ján Kyselovič, Vladimír Frecer, Matthew R. Groves, Zdenko Levarski, André Heeres, Rob H. Henning, Leo E. Deelman   +18 more
wiley   +1 more source

leaves: Advantage of centrifugal partition chromatography over conventional column chromatography

, 2014
International audienceSolanum somalense leaves, used in Djibouti for their medicinal properties, were extracted by MeOH. Because of the high polyphenol and flavonoid contents of the extract, respectively, determined at 80.80 ± 2.13 mg gallic acid ...
Saïda Chideh, Anne Wadouachi, Sylvie Baltora‐Rosset, Dominique Cailleu, Attoumbré, Jacques, Serge Pilard, Pilard, Serge, Saguez, Robert, Hassan-Abdallah, Alshaimaa, Cailleu, Dominique, Robert Saguez, Jacques Attoumbré, Wadouachi, Anne, Chideh, Saïda, Baltora-Rosset, Sylvie, Alshaimaa Hassan‐Abdallah   +15 more
core   +1 more source

Submerged Fermentation of the Edible Mushroom Pleurotus ostreatus in a Batch Stirred Tank Bioreactor as a Promising Alternative for the Effective Production of Bioactive Metabolites

Molecules, 2012
The aim of this study was to investigate the potential of the submerged fermentation procedure in the production of bioactive metabolites of the common edible mushroom Pleurotus ostreatus.
Nikolas Fokialakis, Alexandros-Leandros Skaltsounis, Paul Christakopoulos, Evangelos Topakas, Lefki-Maria Papaspyridi, Nektarios Aligiannis   +5 more
doaj   +1 more source

A posttranslational proteomic survey of a single anatomically preserved human 20‐week postconception brain

Journal of Anatomy, EarlyView.
A well‐preserved 20 post‐conception week human brain was obtained and finely dissected into 18 anatomically distinct regions, including the pia mater. Each region underwent in‐depth proteomic analysis, encompassing both total protein content and post‐translational modifications.
S. Bandiera, H. Bogetofte, P. Jensen, R. Hussain, S. A. Nischal, L. Rihova, G. J. Clowry, M. R. Larsen, Z. Molnár, B. C. Carlyle   +9 more
wiley   +1 more source

Direct Measurement of Octanol-water Partition Coefficients Using Centrifugal Partition Chromatography with a Back-flushing Technique

, 1990
Octanol-water partitioning coefficients (K(oct)) are an accepted parameter in the prediction of biological activity of organic chemicals. Centrifugal Partition Chromatography (CPC), a form of counter current chromatography, is shown to have several ...
Bertrand, Gary L., Menges, Randy A., Armstrong, Daniel W.   +2 more
core   +1 more source

Understanding extracellular vesicle biology, isolation, and characterization: The theory of EV‐erything

Periodontology 2000, EarlyView.
Abstract Background Extracellular vesicles (EVs), especially exosomes, are nanoparticles increasingly recognized as key regulators of intercellular communication in both physiological and pathological aspects. Despite rapid progress, inconsistencies in nomenclature, isolation, and characterization continue to restrict translational advancement.
Paras Ahmad, Nima Farshidfar, Nathan E. Estrin, Yulan Wang, Yufeng Zhang, Anton Sculean, Richard J. Miron   +6 more
wiley   +1 more source

Separation of aflatoxins by centrifugal partition chromatography [PDF]

, 2018
Aflatoxins are mycotoxins, produced by several species of filamentous fungi. For this group of toxins, there are very low, ppb-level limits in the EU in food and feed products, thus as reference standards relatively high amounts of pure aflatoxins are ...
Varga Mónika, Vágvölgyi Csaba, Szekeres András, Endre Gábor, Hegedüs Zsófia   +4 more
core  

A Three Amino Acid Sequence Gates Protein Stability Control of bHLH104 and Iron Uptake in Arabidopsis thaliana

Plant, Cell &Environment, Volume 49, Issue 12, Page 10421-10441, December 2026.
ABSTRACT Iron (Fe) homeostasis is regulated to prevent iron imbalance, with the help of redundant basic helix‐loop‐helix (bHLH) IVc transcription factors that can be controlled by Fe‐binding E3 ligases such as BRUTUS (BTS) in Arabidopsis thaliana.
Dibin Baby, Sarah Weldi, Marie Knopf, Vinitha Venkadasamy, Jessica Lee Erickson, Petra Bauer   +5 more
wiley   +1 more source