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Plant Protoplast Enucleation by Density Gradient Centrifugation
2003A number of different methods have been developed for the enucleation of cells, particularly mammalian cells. Centrifugation of cells adhering to a surface may lead to the nucleus being drawn out from the cytoplasm, resulting in the formation of an enucleated cell or cytoplast and a nucleus surrounded by a thin layer of cytoplasm bounded by a plasma ...
Peter K. Evans +2 more
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Wall effects in sucrose density gradient centrifugation of viruses
Archives of Virology, 1989The depletion of sedimenting particles to be expected from wall effects in the course of sucrose gradient centrifugation, was computed for various types of swing-out rotors on the assumption that particles hitting the tube wall are lost from the gradient. This assumption was roughly verified for heat-denatured, but not for native poliovirus.
Boeye, Albert, De Rees, Alfons
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An improved gradient-making device for density gradient centrifugation
Analytical Biochemistry, 1965Abstract An improved gradient-making device for sucrose density gradient centrifugation is described. The device can be easily constructed with a minimum of glassblowing skill. An alternate mode of operation is presented which allows the production of a linear sucrose density gradient even from a poorly constructed device.
D.M. Kouns, T. Salo
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Automatic optical scanning in density gradient centrifugations
Analytical Biochemistry, 1966SCOPUS: ar.j ; info:eu-repo/semantics ...
Herzog, Albert +2 more
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A Density Gradient Centrifugation Method for Separation of Peat
Separation Science and Technology, 1997The conditions necessary for a laboratory-scale separation of the premaceral constituent of peat (i.e., the precursor to coal) are investigated. The method used is an isopycnic density centrifugation (DGC) technique aimed at isolating pure premacerals.
A. D. Cohen +3 more
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Immuno-ultracentrifugation: A combination of density gradient centrifugation and immunodiffusion
Journal of Immunological Methods, 1977By incorporating acrylamide in a sucrose gradient and photopolymerizing the tubes at the end of the run, direct immunochemical and other analysis can be performed on the separated material in situ without recovering fractions from the centrifuge tube.
P.J. Quinn, M.A. Fernando
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Isopyknotic cushioning for density gradient centrifugation
Experimental Cell Research, 1959G.M. Mateyko, M. J. Kopac
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New cell designs for density gradient centrifugation
Die Makromolekulare Chemie, 1964AbstractThree different designs for double sector cells are presented. The designs allow density gradient experiments to be carried out with equal column lengths in each sector. Also they assure equal heights of the columns from experiment to experiment.
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Quantification with Density Perturbants in Density-Gradient Centrifugation
Biochemical Society Transactions, 1979Norma M. Ryan, Denis R. Headon
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