Results 81 to 90 of about 380,843 (313)
On the isolation of TI-plasmid from Agrobacterium tumefaciens [PDF]
, 1976 An efficient lysis method for Agrobacterium cells was developed, which allows a reproducible isolation of the tumor inducing (TI)-plasmid. The lysis method is based on the sensitivity of this bacterium to incubation with lysozyme, n-dodecylamine,EDTA ...
Dons, Johannes JM +7 more
core +2 more sources
FEBS Open Bio, EarlyView.CAF‐mediated immunosuppression in ovarian cancer is driven by IDO1, reducing T‐cell function. Inhibiting IDO1 restores T‐cell proliferation and cytotoxicity, increases cancer cell apoptosis, and may help overcome CAF‐induced immune suppression in high‐grade serous ovarian cancer. Targeting IDO1 may improve antitumor immunity.
Hyewon Lee +3 more
wiley +1 more source
Preferential localization of a vesicular monoamine transporter to dense core vesicles in PC12 cells. [PDF]
, 1994 Neurons and endocrine cells have two types of secretory vesicle that undergo regulated exocytosis. Large dense core vesicles (LDCVs) store neural peptides whereas small clear synaptic vesicles store classical neurotransmitters such as acetylcholine ...
Edwards, RH +5 more
core
A general model for analysis of linear and hyperbolic enzyme inhibition mechanisms
FEBS Open Bio, EarlyView.We developed a general enzyme kinetic model that integrates these six basic inhibition mechanism onto a single one. From this model, we deduced a general enzyme kinetic equation that through modulation of simple parameters, γ (the relative inhibitor affinity for two binding sites) and β (the reactivity of the enzyme–substrate–inhibitor complex), is ...
Rafael S. Chagas, Sandro R. Marana
wiley +1 more source
Process of infection with bacteriophage phi X 174 XXXVIII. Replication of phi chi 174 replicative form in vivo [PDF]
, 1976 The replication of bacteriophage phi X 174 replicative-form DNA has been studied by structural analysis of pulse-labeled replicative-intermediate molecules.
Fukuda, Akio, Sinsheimer, Robert L.
core
From colloidal dispersions to colloidal pastesthrough solid–liquid separation processes [PDF]
, 2005 Solid–liquid separation is an operation that starts with a dispersion of solid particles in a liquid and removes some of the liquid from the particles, producing a concentrated solid paste and a clean liquid phase.
Botet, Robert +6 more
core +6 more sources
Thrombolytic proteins profiling: High‐throughput activity, selectivity, and resistance assays
FEBS Open Bio, EarlyView.We present optimized biochemical protocols for evaluating thrombolytic proteins, enabling rapid and robust screening of enzymatic activity, inhibition resistance, and fibrin affinity, stimulation, and selectivity. The outcome translates to key clinical indicators such as biological half‐life and bleeding risk. These assays streamline the development of
Martin Toul +3 more
wiley +1 more source
Molecular Therapy: Methods & Clinical Development, 2018 Adeno-associated virus (AAV) is one of the most promising gene therapy vectors and is widely used as a gene delivery vehicle for basic research. As AAV continues to become the vector of choice, it is increasingly important for new researchers to have ...
Sean M. Crosson +3 more
doaj +1 more source
, 2007 Large-scale phosphoproteome analysis on synaptosome and preparation of post-synaptic density (PSD) were investigated. It was found that protein phosphor is a common event in the synapse, which is consistent with the presence of diverse classes of kinases
A Dosemeci +16 more
core +2 more sources
SmallTalk: a novel small‐sized fusion tag for peptide expression and purification
FEBS Open Bio, EarlyView.The SmallTalk fusion tag allows for the efficient expression and purification of soluble recombinant proteins or peptides in Escherichia coli. Testing with SmallTalk‐GFP confirmed that the proteins were soluble and folded correctly, while SmallTalk‐Bin1b maintained its antimicrobial activity against various bacterial isolates. This streamlined workflow
Atika Tariq +3 more
wiley +1 more source