Results 71 to 80 of about 1,124,759 (258)
FEBS Letters, EarlyView.Mycobacterial 3‐methylcrotonyl‐CoA carboxylase uses a mobile biotin‐carrying domain to shuttle a carboxyl group between two catalytic sites, enabling carboxylation of 3‐methylcrotonyl‐CoA during leucine breakdown. Cryo‐electron microscopy captures the carrier at both sites and reveals an inward loop movement that may prevent futile rebinding to the ...
Ajit Yadav +2 more
wiley +1 more source
Sperm quality improvement of cryopreserved boar semen through colloidal centrifugation gradient
Boar semen cryopreservation is limited due to the lower thermal shock resistance of sperm cells. A colloidal centrifugation gradient is an enhancement to cryopreservation that could improve the frozen boar semen quality.
Patricia Cervantes-Acosta +6 more
core +1 more source
Fabrication of Three-Dimensional HepG2 Liver Tissue by Centrifugation
Tokyo Women's Medical University JournalBackground: We previously developed a rapid three-dimensional (3-D) tissue fabrication method using centrifugation. In this study, we fabricated 3-D HepG2 liver tissues using a scaffold-free centrifugation method and analyzed them biochemically. Methods:
Mai Ogawa +2 more
doaj +1 more source
The Shewanella oneidensis Fic enzyme SoFic targets the switch‐I region of EF‐Tu for AMPylation
FEBS Letters, EarlyView.Fic enzymes mediate diverse post‐translational modifications across all domains of life, including AMPylation. Prokaryotic EF‐Tu can be AMPylated and deAMPylated by the conserved Fic enzyme SoFic. Structural and biochemical approaches were used to characterize the effect of AMPylation on EF‐Tu, SoFic's enzymatic activities, and the enzyme‐target ...
Svenja Runge +6 more
wiley +1 more source
, 1994 Soil solution was obtained from potted rhizosphere or non-rhizosphere soils by water displacement or soil centrifugation. The pH of the displaced solutions was lower than that of bulk soils when solutions were obtained from non-rhizosphere soil, although
Lorenz, S. E. +5 more
core +1 more source
Animals(1) Background: Platelet-rich plasma (PRP) therapy has become a cornerstone of equine regenerative medicine, yet significant methodological variability compromises reproducibility and clinical comparability.
Jorge U. Carmona +2 more
doaj +1 more source
FEBS Letters, EarlyView.Synechocystis strains deficient in succinate dehydrogenase (SDH) secrete more succinate than the WT under dark anaerobic conditions, supporting that SDH then primarily acts as SDH, not as a fumarate reductase. L‐aspartate oxidase (Laspo) from Synechocystis is functional under anaerobic conditions, reducing fumarate to succinate.
Kateryna Kukil +3 more
wiley +1 more source
Journal of Fisheries, 2017 The extraction of proteins from wastes reduces production costs and environmental pollution. The aim of this work was to evaluate the effect of two treatments involving decanting/sieving or centrifugation and the number of washing cycles on the quality ...
Lígia Boarin Alcalde +2 more
doaj
Influence of Standard Laboratory Procedures on Measures of Erythrocyte Damage
Frontiers in Physiology, 2017 The ability to characterize the mechanical properties of erythrocytes is important in clinical and research contexts: to diagnose and monitor hematologic disorders, as well as to optimize the design of cardiovascular implants and blood circulating ...
Lena Wiegmann +11 more
doaj +1 more source