The effect of centrifugation speed and time on pre-analytical platelet activation
Clinical Chemistry and Laboratory Medicine, 2016 Background: The results of laboratory analyses are affected by pre-analytical variables, and in particular can platelets be activated by shear handling stress and secrete granular substances.
Anna Cecilia Söderström +3 more
semanticscholar +1 more source
The effect of two pre-cryopreservation single layer colloidal centrifugation protocols in combination with different freezing extenders on the fragmentation dynamics of thawed equine sperm DNA [PDF]
, 2012 Variability among stallions in terms of semen cryopreservation quality renders it difficult to arrive at a standardized cryopreservation method. Different extenders and processing techniques (such us colloidal centrifugation) are used in order to ...
Fernández, Alvaro +11 more
core +1 more source
Neutralization of the PTX3/CD44 axis suppresses TGF‐β1 signaling in kidney fibrosis
FEBS Letters, EarlyView.This study investigates how the PTX3/CD44 axis promotes kidney fibrosis by activating TGF‐β1 signaling. We demonstrate that the neutralizing antibody WHC‐001 effectively inhibits this pathway, suppressing myofibroblast activation and partial epithelial–mesenchymal transition (EMT).
Chao‐Chun Cheng +8 more
wiley +1 more source
Sperm quality improvement of cryopreserved boar semen through colloidal centrifugation gradient
Boar semen cryopreservation is limited due to the lower thermal shock resistance of sperm cells. A colloidal centrifugation gradient is an enhancement to cryopreservation that could improve the frozen boar semen quality.
Patricia Cervantes-Acosta +6 more
core +1 more source
DNA ligase LigE increases bacterial transformation with damaged extracellular DNA
FEBS Letters, EarlyView.The DNA ligase LigE from the naturally competent Gram‐negative bacterium Neisseria gonorrhoaea increases transformation with cut extracellular DNA. This process is ATP‐dependent and relies on the catalytic activity of LigE; it also requires that LigE contains an intact periplasmic localisation sequence, indicating that ligE functions in the periplasm ...
Jolyn Pan +4 more
wiley +1 more source
Effects of Different Centrifugation Parameters on Equilibrium Solubility Measurements
Methods and ProtocolsThe bioavailability of a drug is closely linked to its solubility, making its early determination essential in drug development. The saturation shake-flask (SSF) method is the gold standard protocol for this, which includes a phase separation step—either
Rita Szolláth +4 more
doaj +1 more source
Purification of SWNTs using high-speed centrifugation
, 2008 For eventual commercial applications of single-walled carbon nanotubes (SWNTs) in nanoscale devices, it will be very important to realize effective purification of SWNTs.
Qu YL(曲艳丽) +7 more
core
Guidelines for an optimized differential centrifugation of cells
, 2023 Literature reviews reveal a significant deficiency in conceptual comprehension concerning centrifugation, a crucial step in both medical and research protocols.
Mohammad Sharifian Gh., Fatemeh Norouzi
core +1 more source
Going viral − Virus‐targeting nanobodies for research, diagnostics and therapy
FEBS Letters, EarlyView.The increasing prevalence of viral diseases represents a major threat for global human health. Nanobodies, small binding fragments derived from camelid heavy‐chain antibodies, offer versatile tools for viral research, diagnostics, and antiviral therapy.
Domenic Ebert, Ulrich Rothbauer
wiley +1 more source
Fabrication of Three-Dimensional HepG2 Liver Tissue by Centrifugation
Tokyo Women's Medical University JournalBackground: We previously developed a rapid three-dimensional (3-D) tissue fabrication method using centrifugation. In this study, we fabricated 3-D HepG2 liver tissues using a scaffold-free centrifugation method and analyzed them biochemically. Methods:
Mai Ogawa +2 more
doaj +1 more source