Results 91 to 100 of about 1,676,115 (338)

Valosin‐containing protein counteracts ATP‐driven dissolution of FUS condensates through its ATPase activity in vitro

FEBS Letters, EarlyView.
Biomolecular condensates formed by fused in sarcoma (FUS) are dissolved by high ATP concentrations yet persist in cells. Using a reconstituted system, we demonstrate that valosin‐containing protein (VCP), an AAA+ ATPase, counteracts ATP‐driven dissolution of FUS condensates through its D2 ATPase activity.
Hitomi Kimura, Shin‐ichi Tate, Kyota Yasuda   +2 more
wiley   +1 more source

Comparison of centrifugal-membrane hybrid plasmapheresis and centrifugation therapeutic plasma exchange in hyperlipidemia

Zhongguo shuxue zazhi
[Objective] To establish a new centrifugal-membrane hybrid plasmapheresis (CMHP) model, and observe its clinical efficacy, safety and advantages in the treatment of hyperlipidemia by comparing with centrifugation therapeutic plasma exchange (cTPE ...
ZHANG Bin, WU Chunxi, ZHOU Yumeng, ZHOU Fang, YI Zhongmei   +4 more
doaj   +1 more source

The influence of rotor type and centrifugation time on the yield and purity of extracellular vesicles

Journal of Extracellular Vesicles, 2014
Background Extracellular vesicles (EV), the collective term for vesicles released from cells, consist of vesicle species ranging in size from 30 nm to 5 µm in diameter.
Aleksander Cvjetkovic, J. Lötvall, Cecilia Lässer   +2 more
semanticscholar   +1 more source

Preparation, purification and characterization of aminopropyl-functionalized silica sol [PDF]

, 2013
A new, simple and “green” method was developed for the surface modification of 20 nm diameter Stöber silica particles with 3-aminopropyl(diethoxy)methylsilane in ethanol. The bulk polycondensation of the reagent was inhibited and the stability of the sol
Attila Bóta, Branda, Bruce, Campo, Chuang, Depasse, Deák, Dire, Du, Greena, Guinier, Gábor Tárkányi, Haubold, Ho, Iijima, Iler, Imola Csilla Szigyártó, Judith Mihály, Kneuer, Lee, Lin, Lívia Naszályi Nagy, Marcell Pálmai, Marini, Nagai, Naviroj, Penkova, Pham, Rahman, Réka Mizsei, Salmio, Salon, Shen, Shen, Socrates, Stöber, Teréz Kiss, Thorne, Tibor Kremmer, Vansant, Vejayakumaran, Vrancken, Weigel, Wu, Ye, Yoshida, Yuan, Zoltán Varga   +47 more
core   +1 more source

Hyperosmotic stress induces PARP1‐mediated HPF1‐dependent mono(ADP‐ribosyl)ation

FEBS Letters, EarlyView.
Sorbitol‐induced hyperosmotic stress rapidly induces reversible mono(ADP‐ribosyl)ation (MARylation) on PARP1 without the signs of genotoxic signaling. We show that PARP1 autoMARylation is HPF1 dependent and forms hydroxylamine‐resistant O‐glycosidic linkages.
Anna Georgina Kopasz, Mihály Mérey, Rebeka Vásárhelyi, Ramóna Pék, Victor Imburchia, László Henn, Adrián Kószó, Nicholas D. Lakin, Ivan Ahel, Sébastien Huet, Ágnes Czibula, Gyula Timinszky   +11 more
wiley   +1 more source

Toward Multi-Dimensional Separation of Nanoparticles in Tubular Centrifuges

Powders
The processing and preparation of particulate products is an important process in modern industry and science. The enormous potential for innovation in research and development is due to the complex interactions of solids with their environment.
Marvin Winkler, Marco Gleiss, Hermann Nirschl   +2 more
doaj   +1 more source

Comparative analysis of discrete exosome fractions obtained by differential centrifugation

Journal of Extracellular Vesicles, 2014
Background Cells release a mixture of extracellular vesicles, amongst these exosomes, that differ in size, density and composition. The standard isolation method for exosomes is centrifugation of fluid samples, typically at 100,000×g or above.
D. K. Jeppesen, M. L. Hvam, Bjarke Primdahl-Bengtson, A. Boysen, B. Whitehead, L. Dyrskjøt, T. Ørntoft, K. Howard, M. S. Ostenfeld   +8 more
semanticscholar   +1 more source

An isoform of 14‐3‐3 protein regulates transbilayer lipid movement at the plasma membrane

FEBS Letters, EarlyView.
Loss of 14‐3‐3ζ in CHO cells confers resistance to exogenous phosphatidylserine (PS) and impairs endocytosis‐independent inward flip‐flop of fluorescent PS at the plasma membrane. RNAi‐mediated knockdown reproduces this defect, while no additive effect is seen in ATP11C‐deficient cells.
Akiko Yamaji‐Hasegawa, Akira Hattori, Masafumi Tsujimoto, Toshihide Kobayashi   +3 more
wiley   +1 more source

Minimal Information About Sample Preparation for Phosphoproteomics [PDF]

, 2009
This guideline describes parameters and conditions involved in phosphopeptide sample preparation. It covers from the description and preparation of the cells and tissues to the fractionation and specific enrichment of phosphopeptides for MS analysis. The
David Ovelleiro, Joaquin Abian, Marina Gay, Montserrat Carrascal   +3 more
core   +2 more sources

The ubiquitin ligase RNF115 is required for the clearance of damaged lysosomes

FEBS Letters, EarlyView.
Upon lysosomal rupture, an E3 ubiquitin ligase RNF115 translocates from the cytosol to the damaged lysosomal membrane. Moreover, RNF115 depletion impairs the clearance of damaged lysosomes, identifying it as a key regulator of lysosomal quality control.
Sae Nakanaga, Toshiki Takahashi, Akiko Kuma, Hiroyuki Kawahara   +3 more
wiley   +1 more source