Results 41 to 50 of about 311 (117)
Physiological Reports, Volume 12, Issue 3, February 2024.Abstract Recurrent spontaneous abortion (RSA) is a serious condition that adversely affects women's health. Differentially expressed proteins (DEPs) in plasma of patients experiencing RSA is helpful to find new therapeutic targets and identified with mass spectrometry. In 57 DEPs, 21 were upregulated and 36 were downregulated in RSA.
Li Ji +9 more
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, 2020 Additional file 14 Quantitative PCR (double delta analysis of relative changes in levels of methylated DNA) on CT and KD DNA from human dermal fibroblasts shows widespread differences between CT and KD. (A) qPCR on HpaII-digested DNA (digests DNA flanking unmethylated cytosine) shows a significant (p < 0.05, n = 3 technical replicates, unpaired T ...
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, 2020 Additional file 2. Chemiluminescence scan at a weaker intensity of DBID blots shown in Fig. 3a. The well marked with a red X symbol contains a sample irrelevant to the experiments described. The names of the inhibitors are indicated at the top of each well.
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, 2020 Additional file 3. Tabulation of the sequencing and alignment statistics for CT and KD MeDIP in HEK293T and GM02639.
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, 2020 Additional file 8. CGGBP1 depletion in GM02639 cells: GM02639 cells were transfected with non-targeting or CGGBP1-targeting siRNA twice at 24 and 72 h post-seeding. Cells were harvested at 96 h. Immunoblotting results for CGGBP1 (upper panel) and GAPDH (lower panel) show approximately 55% knockdown of CGGBP1 when normalized to the level of GAPDH.
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, 2020 Additional file 11. Repeat content analysis in HEK293T CT and KD MeDIP DNA shows subfamily-specific methylation changes: Methylation bin frequency plots for HEK293T CT and KD. CT and KD reads for each methylation bin (From 5 to 30) were merged and sequences for merged regions (> 150 bp long) were extracted and subjected to repeat identification. The
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, 2014 Current customers' demands focus on the nutritional and sensory quality of cattle meat. Candidate gene approach allows identification of genetic polymorphisms that have a measurable effect on traits of interest.
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, 2020 Additional file 5. Methylation differences between CT and KD are discernible at small genomic length ranges. Genome-wide methylation signal distribution was compared between CT and KD by using “deeptools multiBigwigSummary”. Methylation signals were compared at bin sizes of 10 kb, 5 kb, 1 kb and 0.2 kb. Correlation between CT and KD was computed by the
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, 2020 Additional file 7. The MeDIP reads distribution for HEK293T CT and KD at low methylation signal bins (1 to 4): Lower methylation signal bins account for a major fraction of MeDIP reads. The frequency of the MeDIP reads for these bins with less than 5 methylation read signals were calculated separately from those in the range 5–30.
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, 2020 Additional file 15. CpG SNPs in the entire AIM dataset and those mapping to the CTCF motifs undergoing AIM in GM02639 cells. The methylation changes at CTCF motifs are not linked to any detectable CpG SNPs within the motifs even if the flanking regions in the sequence reads harbour CpG SNPs.
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