Results 71 to 80 of about 70,973 (282)

Nitrated Nucleotides: New Players in Signaling Pathways of Reactive Nitrogen and Oxygen Species in Plants

Frontiers in Plant Science, 2020
Nitration of diverse biomolecules, including proteins, lipids and nucleic acid, by reactive nitrogen species represents one of the key mechanisms mediating nitric oxide (NO) biological activity across all types of organisms.
Marek Petřivalský, Lenka Luhová
doaj   +1 more source

Continuous Purification of mRNA Produced by In Vitro Transcription Using High Performance Countercurrent Membrane Purification

Biotechnology and Bioengineering, EarlyView.
ABSTRACT Messenger ribonucleic acid (mRNA) therapeutics produced by in vitro transcription must be purified to remove residual enzymes and free nucleotides. This study examines the use of high‐performance countercurrent membrane purification (HPCMP) for the purification of mRNA therapeutics based on differences in the rate of diffusion across ...
Ziqiao Wang, Amin Javidanbardan, Ali Behboudi, Andrew L. Zydney   +3 more
wiley   +1 more source

Activation of HIV-1 expression and replication by cGMP dependent protein kinase type 1-β (PKG1β)

Retrovirology, 2007
The effect of cGMP (cyclic GMP) dependent protein kinase 1-β (PKG1-β) and cGMP analogues on transcriptional activity and replication of human immunodeficiency virus type 1 (HIV-1) was investigated.
Jeang Kuan-Teh, Yedavalli Venkat RK, Lee Jia   +2 more
doaj   +1 more source

Mechanisms associated with cGMP binding and activation of cGMP-dependent protein kinase [PDF]

Proceedings of the National Academy of Sciences, 2003
Using small-angle x-ray scattering, we have observed the cGMP-induced elongation of an active, cGMP-dependent, monomeric deletion mutant of cGMP-dependent protein kinase (Δ 1–52 PKG-Iβ). On saturation with cGMP, the radius of gyration of Δ 1–52 PKG-Iβ increases from 29.4 ± 0.1 Å to 40.1 ± 0.7
Michael E, Wall, Sharron H, Francis, Jackie D, Corbin, Kennard, Grimes, Robyn, Richie-Jannetta, Jun, Kotera, Brian A, Macdonald, Rowena R, Gibson, Jill, Trewhella   +8 more
openaire   +2 more sources

Glycoengineered Recombinant Alpha1‐Antitrypsin Results in Comparable In Vitro and In Vivo Activities to Human Plasma‐Derived Protein

Biotechnology and Bioengineering, EarlyView.
ABSTRACT Alpha‐1‐antitrypsin (A1AT) is a multifunctional, clinically important, high‐value therapeutic glycoprotein that can be used for the treatment of many diseases, such as A1AT deficiency, diabetes, graft‐versus‐host disease, cystic fibrosis, and various viral infections. Currently, the only U.S. food and drug administration‐approved treatment for
Frances Rocamora, Chen‐Lin Hsieh, Sanne Schoffelen, Johnny Arnsdorf, Eric A. Toth, Abdul S. Yunus, Thomas E. Cleveland IV, Sara Petersen Bjørn, Mina Ying Min Wu, Noel G. McElvaney, Bjørn Gunnar Rude Voldborg, Thomas R. Fuerst, Nathan E. Lewis   +12 more
wiley   +1 more source

Phosphodiesterase 5 attenuates the vasodilatory response in renovascular hypertension.

PLoS ONE, 2013
NO/cGMP signaling plays an important role in vascular relaxation and regulation of blood pressure. The key enzyme in the cascade, the NO-stimulated cGMP-forming guanylyl cyclase exists in two enzymatically indistinguishable isoforms (NO-GC1, NO-GC2) with
Johannes Stegbauer, Sebastian Friedrich, Sebastian A Potthoff, Kathrin Broekmans, Miriam M Cortese-Krott, Ivo Quack, Lars Christian Rump, Doris Koesling, Evanthia Mergia   +8 more
doaj   +1 more source

Interconvertible cGMP-free and cGMP-bound forms of cGMP-dependent protein kinase in mammalian tissues.

Journal of Biological Chemistry, 1987
Several vascular and nonvascular mammalian tissue extracts exhibited variable amounts of two peaks (peaks I and II) of cGMP-dependent protein kinase by NaCl elution of DEAE columns. When [3H]cGMP was added to the extracts before chromatography, a peak of protein-bound [3H]cGMP coeluted with peak II.
L, Wolfe, S H, Francis, L R, Landiss, J D, Corbin   +3 more
openaire   +2 more sources

Partial Reconstitution of Photoreceptor cGMP Phosphodiesterase Characteristics in cGMP Phosphodiesterase-5 [PDF]

Journal of Biological Chemistry, 2001
Photoreceptor cGMP phosphodiesterases (PDE6) are uniquely qualified to serve as effector enzymes in the vertebrate visual transduction cascade. In the dark-adapted photoreceptors, the activity of PDE6 is blocked via tight association with the inhibitory gamma-subunits (Pgamma).
A E, Granovsky, N O, Artemyev
openaire   +2 more sources

Advances and Techniques to Measure cGMP in Intact Cardiomyocytes

, 2013
Förster resonance energy transfer (FRET)-based biosensors are powerful tools for real-time monitoring of signaling events in intact cells using fluorescence microscopy.
Götz, Konrad R., Krieg, Thomas, Lukowski, Robert, Nikolaev, Viacheslav O.   +3 more
core   +1 more source

Status and future of recombinant adeno‐associated virus vector manufacturing

Biotechnology Progress, EarlyView.
Abstract Sixty years of adeno‐associated virus (AAV) research illustrates a trajectory marked by basic science exploration, iterative innovation, persistent challenges, a number of clinical setbacks, as well as commercial therapeutic triumphs. This continual evolution has led to recombinant AAV (rAAV) becoming a cornerstone of modern gene therapy ...
Frank Agbogbo, David Dismuke
wiley   +1 more source