Results 131 to 140 of about 18,335 (296)

Organization of Valence-Encoding and Projection-Defined Neurons in the Basolateral Amygdala

Cell Reports, 2018
The basolateral amygdala (BLA) mediates associative learning for both fear and reward. Accumulating evidence supports the notion that different BLA projections distinctly alter motivated behavior, including projections to the nucleus accumbens (NAc ...
Anna Beyeler, Chia-Jung Chang, Margaux Silvestre, Clémentine Lévêque, Praneeth Namburi, Craig P. Wildes, Kay M. Tye   +6 more
doaj   +1 more source

Hippocampal low-frequency stimulation prevents seizure generation in a mouse model of mesial temporal lobe epilepsy

eLife, 2020
Mesial temporal lobe epilepsy (MTLE) is the most common form of focal, pharmacoresistant epilepsy in adults and is often associated with hippocampal sclerosis. Here, we established the efficacy of optogenetic and electrical low-frequency stimulation (LFS)
Enya Paschen, Claudio Elgueta, Katharina Heining, Diego M Vieira, Piret Kleis, Catarina Orcinha, Ute Häussler, Marlene Bartos, Ulrich Egert, Philipp Janz, Carola A Haas   +10 more
doaj   +1 more source

Three-dimensional scanless holographic optogenetics with temporal focusing (3D-SHOT). [PDF]

, 2017
Optical methods capable of manipulating neural activity with cellular resolution and millisecond precision in three dimensions will accelerate the pace of neuroscience research. Existing approaches for targeting individual neurons, however, fall short of
Adesnik, Hillel, Mardinly, Alan, Oldenburg, Ian, Pégard, Nicolas, Sridharan, Savitha, Waller, Laura   +5 more
core   +2 more sources

Multidimensional screening yields channelrhodopsin variants having improved photocurrent and order-of-magnitude reductions in calcium and proton currents

Journal of Biological Chemistry, 2019
Channelrhodopsins (ChRs) are light-gated ion channels in widespread use in neuroscience for mediating the genetically targetable optical control of neurons (optogenetics).
Y. Cho, Demian Park, A. Yang, Fei Chen, A. Chuong, Nathan C. Klapoetke, E. Boyden   +6 more
semanticscholar   +1 more source

Direct Stimulation of Gastric Smooth Muscle Cells via Gq Proteins With Light

Neurogastroenterology &Motility, EarlyView.
Optogenetic stimulation enables direct and selective stimulation of smooth muscle cells and thus provides a new opportunity to restore gastric contractility in cases of gastroparesis. Herein, we demonstrate that hOPN5 is a new and effective tool acting via Gq signaling and is thus complementary to light‐induced membrane depolarization with ...
David Zipf, Markus Vogt, Udhayabhaskar Sathyanarayanan, Ahmed Wagdi, Johannes Riebeling, Robert Patejdl, Tobias Bruegmann   +6 more
wiley   +1 more source

The two-photon reversible reaction of the bistable jumping spider rhodopsin-1 [PDF]

, 2019
Bistable opsins are photopigments expressed in both invertebrates and vertebrates. These light-sensitive G-protein-coupled receptors undergo a reversible reaction upon illumination.
Deupi, Xavier, Ehrenberg, David, Heberle, Joachim, Koyanagi, Mitsumasa, Lesca, Elena, Schertler, Gebhard F. X., Terakita, Akihisa, Varma, Niranjan   +7 more
core   +1 more source

Nonadiabatic Photodynamics of Retinal Protonated Schiff Base in Channelrhodopsin 2.

Journal of Physical Chemistry Letters, 2019
Channelrhodopsin 2 (ChR2) is a light-gated ion channel and an important tool in optogenetics. Photoisomerization of retinal protonated Schiff base (RPSB) in ChR2 triggers channel activation.
Ruibin Liang, Fang Liu, T. Martínez
semanticscholar   +1 more source

Kinetics of proton release and uptake by channelrhodopsin‐2 [PDF]

FEBS Letters, 2012
Electrophysiological experiments showed that the light‐activated cation channel channelrhodopsin‐2 (ChR2) pumps protons in the absence of a membrane potential. We determined here the kinetics of transient pH change using a water‐soluble pH‐indicator.
M. Nack, l. Radu, B. J. Schultz, T. Resler, R. Schlesinger, A. N. Bondar, C. del Val, C. Bamann, E. Bamberg, J. Heberle, ABBRUZZETTI, Stefania, VIAPPIANI, Cristiano   +11 more
openaire   +5 more sources

Optogenetic methods to stimulate gamma motor neuron axons ex vivo

Experimental Physiology, EarlyView.
Abstract It is challenging to stimulate gamma motor neurons, which are important regulators of muscle spindle afferent function, without also recruiting alpha motor neurons. Here, we test the feasibility of stimulating gamma motor neuron axons using optogenetics in two transgenic mouse lines. We used an ex vivo muscle–nerve preparation in adult mice to
Apoorva Karekal, Remie Mandawe, Cameron Chun, Sai Kiran Byri, Danitza Cheline, Serena Ortiz, Shawn Hochman, Katherine A. Wilkinson   +7 more
wiley   +1 more source

Control of voluntary and optogenetically perturbed locomotion by spike rate and timing of neurons of the mouse cerebellar nuclei

eLife, 2018
Neurons of the cerebellar nuclei (CbN), which generate cerebellar output, are inhibited by Purkinje cells. With extracellular recordings during voluntary locomotion in head-fixed mice, we tested how the rate and coherence of inhibition influence CbN cell
Rashmi Sarnaik, Indira M Raman
doaj   +1 more source