Results 51 to 60 of about 139,168 (286)

Screening and epitope characterization of Nidogen‐2‐specific nanobodies

open access: yesFEBS Open Bio, EarlyView.
Camel immunization and phage display were employed to generate high‐affinity VHH nanobodies against Nidogen‐2. After library construction, biopanning, ELISA screening, sequencing, and recombinant expression, selected nanobodies were purified and characterized, leading to the preliminary exploration of a nanobody‐based sandwich ELISA for specific ...
Jianchuan Wen   +9 more
wiley   +1 more source

Evaluation of multiple generative large language models on neurology board-style questions

open access: yesFrontiers in Digital Health
ObjectiveTo compare the performance of eight large language models (LLMs) with neurology residents on board-style multiple-choice questions across seven subspecialties and two cognitive levels.MethodsIn a cross-sectional benchmarking study, we evaluated ...
Mohammad Almomani   +9 more
doaj   +1 more source

"ChatGPT, a Friend or Foe for Education?" Analyzing the User's Perspectives on the Latest AI Chatbot Via Reddit

open access: yes, 2023
Latest developments in Artificial Intelligence (AI) and big data gave rise to Artificial Intelligent agents like Open AI's ChatGPT, which has recently become the fastest growing application since Facebook and WhatsApp.
Ayinde, Lateef   +3 more
core  

Proteasomal degradation of intracellularly expressed Amblyomin‐X limits suicide gene therapy potential in melanoma cells

open access: yesFEBS Open Bio, EarlyView.
This study explores the feasibility of expressing the antitumoral protein Amblyomin‐X through a suicide gene therapy approach and investigates its intracellular fate after gene delivery. Although the gene is efficiently expressed, melanoma cells rapidly degrade the Amblyomin‐X protein via proteasome activity.
Victor Dal Posolo Cinel   +4 more
wiley   +1 more source

ChatGPT in psychiatry: promises and pitfalls

open access: yesThe Egyptian Journal of Neurology, Psychiatry and Neurosurgery
ChatGPT has become a hot topic of discussion since its release in November 2022. The number of publications on the potential applications of ChatGPT in various fields is on the rise.
Rebecca Shin-Yee Wong
doaj   +1 more source

Generative AI, Why, How, and Outcomes: A User Adoption Study [PDF]

open access: yes
Drawing on the extended unified theory of acceptance and use of technology (UTAUT2) and task-technology fit (TTF) theory, we developed an integrated research model to explore the factors affecting ChatGPT use and its subsequent effects on whether users ...
Huy, Le Van   +4 more
core   +2 more sources

Rapid screening of staphylokinase protein variants using an unpurified cell‐free expression system

open access: yesFEBS Open Bio, EarlyView.
An unpurified cell‐free protein synthesis (CFPS) platform enables rapid functional screening of staphylokinase variants. Direct plasminogen‐activation assays performed in microplate format provide real‐time activity readouts, allowing rapid identification and ranking of variants with improved or reduced fibrinolytic activity without protein ...
Maria Tomková   +3 more
wiley   +1 more source

Insights into nurse educators' use of ChatGPT in active teaching methods: A cross-sectional pilot study

open access: yesEducación Médica
Introduction: Artificial intelligence has revolutionized nursing education with the emergence of ChatGPT, which has the potential to transform active teaching methods.
Asma Bouriami   +5 more
doaj   +1 more source

Pharmacological inhibition of the PERK pathway modulates hepatocellular carcinoma growth and immune signaling

open access: yesFEBS Open Bio, EarlyView.
Pharmacological inhibition of PERK in a DEN‐induced mouse model of liver cancer does not reduce tumor burden but alters cellular stress signaling. Despite blocking PERK activity, downstream stress responses, including CHOP expression, remain active, suggesting compensatory mechanisms within the unfolded protein response that may influence tumor ...
Ada Lerma‐Clavero   +5 more
wiley   +1 more source

Knowledge, Attitude, and Practices of General Population Toward Utilizing ChatGPT: A Cross-sectional Study

open access: yesSAGE Open, 2023
ChatGPT, an artificial intelligence-based program, has been used by numerous users in various fields. This study aimed to determine to knowledge, attitude, and practices of the general population toward the use of ChatGPT.
Nikita Bodani   +5 more
doaj   +1 more source

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