Results 81 to 90 of about 1,834,987 (265)

Structural and biochemical analysis of a B12 superbinder

FEBS Letters, EarlyView.
BtuG proteins are vitamin B12 scavengers in Bacteroides thetaiotaomicron, a dominant human gut bacterium. We present crystal structures of three BtuG homologs bound to cobalamin and its precursor cobinamide, revealing picomolar binding affinities, among the highest known for any natural protein.
Jose M. Martinez Felices, Jacob J. Whittaker, Dirk J. Slotboom, Albert Guskov   +3 more
wiley   +1 more source

Structures of mycobacterial 3‐methylcrotonyl‐CoA carboxylase reveal carrier‐domain translocation between catalytic sites

FEBS Letters, EarlyView.
Mycobacterial 3‐methylcrotonyl‐CoA carboxylase uses a mobile biotin‐carrying domain to shuttle a carboxyl group between two catalytic sites, enabling carboxylation of 3‐methylcrotonyl‐CoA during leucine breakdown. Cryo‐electron microscopy captures the carrier at both sites and reveals an inward loop movement that may prevent futile rebinding to the ...
Ajit Yadav, Bogdan I. Florea, Sebastian Geibel   +2 more
wiley   +1 more source

The crystal structure of 4-carboxy-2-oxobutan-1-aminium chloride, C5H10ClNO3

Zeitschrift für Kristallographie - New Crystal Structures
C5H10ClNO3, orthorhombic, Pbca (no. 61), a = 8.2012(1) Å, b = 11.2215(2) Å, c = 16.8339(3) Å, V = 1549.22(4) Å3, Z = 8, Rgt(F) = 0.0473, wRref(F2) = 0.1331, T = 297 K.
Wang Fengfeng, Zhang Caiyu, Wang Youyou, Luan Lin, Hu Xiaowen, Liu Yang   +5 more
doaj   +1 more source

From junk to function — How weak selection in eukaryotes builds new parts and drives genomic complexity

FEBS Letters, EarlyView.
How do genomes gain new functional parts? In eukaryotes, which tend to evolve under weak selection, much of the genome is junk. Palazzo and Qiu borrow the logic of Markov chains to show how non‐functional DNA becomes functional through the appearance of intermediate states, which arise due to epistasis, buffering, and biochemical messiness, allowing ...
Alexander F. Palazzo, Yi Qiu
wiley   +1 more source

Fractional order system identification using a joint multi-innovation fractional gradient descent algorithm

Scientific Reports
This paper proposes a joint multi-innovation fractional gradient descent identification algorithm for fractional order systems. First, the flexibility of fractional calculus is leveraged to design a joint fractional gradient descent algorithm capable of ...
Zishuo Wang, Beichen Chen, Hongliang Sun, Shuning Liang   +3 more
doaj   +1 more source

The Shewanella oneidensis Fic enzyme SoFic targets the switch‐I region of EF‐Tu for AMPylation

FEBS Letters, EarlyView.
Fic enzymes mediate diverse post‐translational modifications across all domains of life, including AMPylation. Prokaryotic EF‐Tu can be AMPylated and deAMPylated by the conserved Fic enzyme SoFic. Structural and biochemical approaches were used to characterize the effect of AMPylation on EF‐Tu, SoFic's enzymatic activities, and the enzyme‐target ...
Svenja Runge, Vivian Pogenberg, Alexander Baumgart, Bente Siebels, Hartmut Schlüter, Michael Hecht‐Bucher, Aymelt Itzen   +6 more
wiley   +1 more source

Additional fractional gradient descent identification algorithm based on multi-innovation principle for autoregressive exogenous models

Scientific Reports
This paper proposed the additional fractional gradient descent identification algorithm based on the multi-innovation principle for autoregressive exogenous models.
Zishuo Wang, Shuning Liang, Beichen Chen, Hongliang Sun   +3 more
doaj   +1 more source

Prospecting the protein design landscape

FEBS Letters, EarlyView.
This review outlines the current state of various protein design approaches. We discuss the current possibilities enabled by recently released tools, highlight future avenues to pursue in protein design, and underscore the crucial role of key databases and resources for successful protein design workflows.
Jakob R. Riccabona, Katharina T. Stonig, Jens Meiler, Clara T. Schoeder, Monica L. Fernández‐Quintero   +4 more
wiley   +1 more source

Tecovirimat-Related Substance: Characterization, Hirshfeld Analysis, Theoretical Study, In Silico Toxicity Assessment

Molecules
Crystal structures of 4-(trifluoromethyl)benzene-1-carbohydrazide (1), 4-(trifluoromethyl)-N’-{[4-(trifluoromethyl)phenyl]carbonyl}benzohydrazide (2) and (1R,2S,6R,7S,8S,10R)-4-({[4-(trifluoromethyl)phenyl]carbonyl}amino)-4-azatetracyclo [5.3.2.08,10.02 ...
Fengfeng Wang, Xiaowen Hu, Caiyu Zhang, Lin Luan, Zhengzheng Zhou, Yang Liu   +5 more
doaj   +1 more source

L‐aspartate oxidase provides new insights into fumarate reduction in anaerobic darkness in Synechocystis sp. PCC6803

FEBS Letters, EarlyView.
Synechocystis strains deficient in succinate dehydrogenase (SDH) secrete more succinate than the WT under dark anaerobic conditions, supporting that SDH then primarily acts as SDH, not as a fumarate reductase. L‐aspartate oxidase (Laspo) from Synechocystis is functional under anaerobic conditions, reducing fumarate to succinate.
Kateryna Kukil, Jeffrey A. Hawkes, Cecilia Blikstad, Pia Lindberg   +3 more
wiley   +1 more source