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Chemiluminescent labels for steroid immunoassays
Journal of Steroid Biochemistry, 1987During the last few years non-isotopic techniques have been developed to measure steroid hormones. One of these methods is the luminescence immunoassay. Three classes of substances have been found to yield reasonable results, i.e. aminophthalhydrazides, aminonaphthohydrazides and acridiniumesters. Examples of application are shown.
W, Klingler, G, Wiegand, R, Knuppen
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Enhanced chemiluminescent immunoassay for aldosterone
Journal of Bioluminescence and Chemiluminescence, 1990AbstractA solid phase immunoassay for aldosterone using enhanced chemiluminescent detection has been developed. Monoclonal antibodies against aldosterone were used for the immune reaction and compared with polyclonal antibodies. Uniform Protein A coated polystyrene tubes were used as solid phase for the monoclonal antibody and second (anti‐rabbit ...
W, Hubl +4 more
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Chemiluminescence immunoassay in microemulsions
Colloids and Surfaces B: Biointerfaces, 1998Abstract A novel chemiluminescence immunoassay which combines microparticles and microemulsion is described. The method is based on a specific interaction of an antibody with an antigen preadsorbed onto fluorescer (perylene) microparticles (mean diameter 0.8–1.6 μm).
Alexander Kamyshny, Shlomo Magdassi
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Chemiluminescence to Immunoassays
2010The extreme sensitivity of chemiluminescence analysis in combination of the high specificity of antibodies has resulted in many application of chemiluminescence immunoassays (CLIA) over the last three decades. Especially the reproducible analysis in complex matrices leads to the success of this method in clinical, food and environmental analysis ...
Michael Seidel, Reinhard Niessner
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An immunoassay for plasma cortisol based on chemiluminescence
Steroids, 1980An immunoassay procedure for the determination of cortisol in human plasma is described, which utilizes chemiluminescence as the end point. A cortisol-isoluminol conjugate serves as the chemiluminescent marker. The light emission by this conjugate upon oxidation is delayed by prior incubation with anti-cortisol IgG, but not by unrelated gamma-globulin.
F. Kohen +3 more
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A chemiluminescence immunoassay for the detection of NT-proBNP
Analytical Biochemistry, 2020The aim of the study was to assess the analytical performance of the HISCL NT-proBNP assay, a newly developed chemiluminescence immunoassay, for the detection of NT-proBNP.The within-run and total imprecision of the NT-proBNP assay were determined with HISCL cardiac marker controls.
Yu-Yan, Chen +5 more
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Advent of Innovative Chemiluminescent Enzyme Immunoassay
Biosensors and Bioelectronics, 2010Using 1,1'-oxalyldiimidazole (ODI) chemiluminescence detection, a new chemiluminescent enzyme immunoassay (CLEIA) was developed to quantify prostate specific antigen (PSA) in human serum. The results observed in ODI CLEIA were compared with those obtained in commercially available enzyme linked immunosorbent assay (ELISA), fluorescence enzyme ...
Ji Hoon Lee
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Chemiluminescent tags in immunoassays
TrAC Trends in Analytical Chemistry, 1983Abstract Although radioimmunoassay procedures have a number of advantages they do pose problems, especially with regard to the disposal of radioactive waste. Alternative methods, such as chemiluminescence, enzyme, fluorescence, or spin immunoassays, have been tested with the aim of replacing radioactive labels without loss of sensitivity, precision ...
W. Klingler, C.J. Strasburger, W.G. Wood
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Chemiluminescent immunoassay (CLIA) for urinary albumin
Journal of Bioluminescence and Chemiluminescence, 1989AbstractA simple chemiluminescent immunoassay (CLIA) for urinary albumin has been developed based on the use of a chemiluminescent acridinium ester‐labelled human albumin and a commercially available antiserum. It includes two incubation steps and a second polyethylene glycol‐assisted antibody separation. The sensitivity of detection is 0.016 mg/l, the
F J, Bagazgoitia +6 more
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Chemiluminescence enzyme immunoassay for the determination of sulfamethoxydiazine
Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy, 2011Sulfamethoxydiazine (SMD), which is often used for animal disease treatment, is harmful to human health. No SMD residue should be detected in food in some countries, such as USA and Japan. Therefore, it is significant to develop a high-throughput, high-sensitivity and accurate method for the determination of the content of SMD in food.
Yongjun, Wu +5 more
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