The agrin gene codes for a family of basal lamina proteins that differ in function and distribution [PDF]
We isolated two cDNAs that encode isoforms of agrin, the basal lamina protein that mediates the motor neuron-induced aggregation of acetylcholine receptors on muscle fibers at the neuromuscular junction.
Tsim, K. W. +13 more
core +1 more source
Biphasic effect of pentobarbitone on chick myotube nicotinic receptor channel kinetics [PDF]
The modulatory action of pentobarbitone on chick myotube nicotinic acetylcholine receptor kinetics was studied by the patch clamp technique, particularly focussing on effects at low concentrations. Open time sojourn distributions of foetal‐type receptors recorded at room temperature (22–24°C) in cell‐attached mode in the presence of 0.2 μm ...
G J, Liu, B W, Madsen
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Effects of Serum Deprivation on Myofibrillar Proteolysis in Chick Myotube Cultures
We examined the effects of serum deprivation on myofibrillar proteolysis in chick myotubes. Myotubes were incubated with serum-free medium for 24 hours. N(tau)-methylhistidine release, as an index of myofibrillar proteolysis, as well as protease activities such as calpain, proteasome, and cathepsins (B+L and D) activities were increased by serum ...
NAKASHIMA, Kazuki +2 more
openaire +2 more sources
The differentiation of chick embryonic myoblasts in primary culture [PDF]
The differentiation of chick embryonic skeletal myoblasts results in the formation of myotubes which are the precursors of muscle fibres. The fusion of mononucleated myoblasts represents an apparent switching point in differentiation since it results in ...
Evans, Caroline Akrill
core +1 more source
Enhanced chemosensitivity of chick parasympathetic neurones in co‐culture with myotubes.
1. The influence of target interaction upon the electrophysiological properties of dissociated ciliary ganglion cells was investigated by testing the sensitivity of the neuronal somal membrane to ionophoretically applied acetylcholine (ACh). Variations in the percentage of cells responsive to the transmitter were measured with time in culture.
G, Crean, G, Pilar, J B, Tuttle, K, Vaca
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Purification and characterization of a polypeptide from chick brain that promotes the accumulation of acetylcholine receptors in chick myotubes. [PDF]
Acetylcholine receptors (AChRs) are packed in the postsynaptic membrane at neuromuscular junctions at a density of approximately 20,000/micron 2, whereas the density a few micrometers away is less than 20/micron 2. To understand how this remarkable distribution comes about during nerve-muscle synapse formation, we have attempted to isolate factors from
T B, Usdin, G D, Fischbach
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Effects of Serum Deprivation on Expression of Proteolytic-Related Genes in Chick Myotube Cultures [PDF]
We previously reported that serum deprivation stimulates myofibrillar proteolysis in chick myotubes. In the present study, we examined the effect of serum deprivation on expression of the proteolytic-related genes (ubiquitin, proteasome, calpains, and cathepsin B) by real-time PCR of cDNA in chick myotubes.
NAKASHIMA, Kazuki +2 more
openaire +2 more sources
Repeated disuse imprints a molecular memory in skeletal muscle, conferring transcriptional resilience in young adults but exaggerated susceptibility in aged muscle, driven by epigenetic regulation of aerobic metabolism, mitochondrial and NAD+ pathways.
Daniel C. Turner +14 more
wiley +1 more source
Micro Pattern‐Based 3D Cell Culture Platform: An Overview of Technologies and Applications
Micro patterns are characterized as a coating of specific adhesion matrices on substrates to support cell growth. An overview of micropattern technology to regulate single cell morphology and fates, further focusing on the micro pattern‐based 3D cell culture platform used in the non‐tumor and tumor researches.
Xinglong Zhu +8 more
wiley +1 more source
The Space Within: How Architected Voids Promote Tissue Formation
This review explores the role of void spaces in tissue engineering scaffolds and examines four key methods for introducing porosity into hydrogels at different scales. It discusses sacrificial templating, microgels, phase separation, and 3D printing, highlighting principles, advantages, and limitations. It also addresses emerging strategies integrating
Anna Puiggalí‐Jou +3 more
wiley +1 more source

