cDNA that encodes active agrin [PDF]
Agrin is thought to mediate the motor neuron-induced aggregation of AChRs and AChE on the surface of muscle fibers at neuromuscular junctions. We have isolated a cDNA from a chick brain library that, based on sequence homology and expression experiments,
Kröger, Stephan +9 more
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Permeability properties of chick myotube acetylcholine-activated channels
The acetylcholine-(ACh-)activated channels of chick myotubes were studied by the patch-clamp method. Single-channel amplitudes were measured over a wide range of potentials in solutions of cesium, arginine, and three small amines. Symmetrical, isotonic cesium solutions gave a linear I-V relationship with the single-channel conductance, gamma, of 42 pS ...
Dwyer, T.M., Farley, J.M.
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The agrin gene codes for a family of basal lamina proteins that differ in function and distribution [PDF]
We isolated two cDNAs that encode isoforms of agrin, the basal lamina protein that mediates the motor neuron-induced aggregation of acetylcholine receptors on muscle fibers at the neuromuscular junction.
Tsim, K. W. +13 more
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Biphasic effect of pentobarbitone on chick myotube nicotinic receptor channel kinetics [PDF]
The modulatory action of pentobarbitone on chick myotube nicotinic acetylcholine receptor kinetics was studied by the patch clamp technique, particularly focussing on effects at low concentrations. Open time sojourn distributions of foetal‐type receptors recorded at room temperature (22–24°C) in cell‐attached mode in the presence of 0.2 μm ...
G J, Liu, B W, Madsen
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Effects of Serum Deprivation on Myofibrillar Proteolysis in Chick Myotube Cultures
We examined the effects of serum deprivation on myofibrillar proteolysis in chick myotubes. Myotubes were incubated with serum-free medium for 24 hours. N(tau)-methylhistidine release, as an index of myofibrillar proteolysis, as well as protease activities such as calpain, proteasome, and cathepsins (B+L and D) activities were increased by serum ...
NAKASHIMA, Kazuki +2 more
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Prospects for transgenesis in the chick [PDF]
Research to develop a useful method for genetic modification of the chick has been on-going since the first demonstrations in the mouse in the 1980s that genetic modification is an invaluable tool for the study of gene function. Manipulation of the chick
Sang, Helen
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Enhanced chemosensitivity of chick parasympathetic neurones in co‐culture with myotubes.
1. The influence of target interaction upon the electrophysiological properties of dissociated ciliary ganglion cells was investigated by testing the sensitivity of the neuronal somal membrane to ionophoretically applied acetylcholine (ACh). Variations in the percentage of cells responsive to the transmitter were measured with time in culture.
G, Crean, G, Pilar, J B, Tuttle, K, Vaca
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Effects of High Salt-Exposure on the Development of Retina and Lens in 5.5-Day Chick Embryo [PDF]
Background/Aims: Excess maternal salt intake during pregnancy may alter fetal development. However, our knowledge on how an increased salt intake during pregnancy influences fetal eye development is limited.
Xiao-yu Wang +18 more
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Purification and characterization of a polypeptide from chick brain that promotes the accumulation of acetylcholine receptors in chick myotubes. [PDF]
Acetylcholine receptors (AChRs) are packed in the postsynaptic membrane at neuromuscular junctions at a density of approximately 20,000/micron 2, whereas the density a few micrometers away is less than 20/micron 2. To understand how this remarkable distribution comes about during nerve-muscle synapse formation, we have attempted to isolate factors from
T B, Usdin, G D, Fischbach
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Excess caffeine exposure impairs eye development during chick embryogenesis [PDF]
Caffeine has been an integral component of our diet and medicines for centuries. It is now known that over consumption of caffeine has detrimental effects on our health, and also disrupts normal foetal development in pregnant mothers.
Chuai, Manli +6 more
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