Regulation of Autophagy in Chick Myotubes: Effects of Insulin, Insulin-Like Growth Factor-I, and Amino Acids [PDF]
Journal of Poultry Science, 2018 Autophagy, an intracellular bulk protein degradation system in skeletal muscle, is increased under catabolic conditions resulting in muscle atrophy. This study aimed to investigate the effects of insulin, insulin-like growth factor (IGF)-I, and amino ...
Kazuki Nakashima
exaly +5 more sources
Myostatin Increases Smad2 Phosphorylation and Atrogin-1 Expression in Chick Embryonic Myotubes [PDF]
Journal of Poultry Science, 2019 Skeletal muscle mass is an important trait in poultry meat production. In mammals, myostatin, a negative regulator of skeletal muscle growth, activates Smad transcription factors and induces the expression of atrogin-1 by regulating the Akt/FOXO pathway.
Takaoki Saneyasu +2 more
exaly +7 more sources
Regulation of autophagy in chick myotube cultures: Effect of uncoupling mitochondrial oxidative phosphorylation. [PDF]
J Poult Sci, 2023 s: Skeletal muscles have a high demand for ATP, which is met largely through mitochondria oxidative phosphorylation. Autophagy is essential for the maintenance of skeletal muscle mass under catabolic conditions.
Nakashima K, Ishida A.
europepmc +4 more sources
Insulin Stimulation of Protein Synthesis and mTOR Signaling in Chick Myotube Cultures
The Journal of Poultry Science, 2020 Insulin stimulates protein synthesis in skeletal muscles. Protein synthesis is controlled by the mechanistic target of rapamycin (mTOR) signaling in skeletal muscles.
Kazuki Nakashima +4 more
doaj +5 more sources
The Wnt/Beta-Catenin Pathway and Cytoskeletal Filaments Are Involved in the Positioning, Size, and Function of Lysosomes during Chick Myogenesis. [PDF]
Cells, 2022 Lysosomes are highly dynamic organelles involved in the breakdown and recycling of macromolecules, cell cycle, cell differentiation, and cell death, among many other functions in eukaryotic cells. Recently, lysosomes have been identified as cellular hubs
Bagri KM +4 more
europepmc +2 more sources
The ensured proliferative capacity of myoblast in serum-reduced conditions with Methyl-β-cyclodextrin. [PDF]
Front Cell Dev Biol, 2023 To produce muscle fibers for cultured meat on a large scale, it is important to expand myoblasts in a serum-reduced or serum-free medium to avoid cost, ethical, and environmental issues.
Katayama T, Chigi Y, Okamura D.
europepmc +2 more sources
The Journal of Poultry Science, 2022 Protein synthesis in skeletal muscle is considered one of the most energy-consuming cellular processes. AMP-activated protein kinase (AMPK) is a metabolic master switch that regulates glucose and lipid metabolism, and it is implicated in protein ...
Kazuki Nakashima, Aiko Ishida
doaj +2 more sources
The Journal of Poultry Science, 2017 The expression of atrogin-1/MAFbx, a muscle-specific E3 ubiquitin ligase, is increased in catabolic conditions that result in muscle atrophy. The expression of atrogin-1/MAFbx mRNA is also decreased by the insulin-like growth factor-I (IGF-I) in ...
Kazuki Nakashima +4 more
doaj +2 more sources
Chick embryo extract-driven culture system enhances chicken muscle satellite cell differentiation and 3D myotube formation. [PDF]
NPJ Sci FoodThe rapid increase in global meat demand has placed significant pressure on traditional meat production. This has led to the emergence of the cultured meat industry as a promising alternative offering sustainable and ethical solutions.
Kang Y, Lee J, Park J, Do JT.
europepmc +2 more sources
Microbiota-derived 3-phenylpropionic acid promotes myotube hypertrophy by Foxo3/NAD+ signaling pathway. [PDF]
Cell BiosciBackground Gut microbiota and their metabolites play a regulatory role in skeletal muscle growth and development, which be known as gut-muscle axis. 3-phenylpropionic acid (3-PPA), a metabolite produced by colonic microorganisms from phenylalanine in the
Li P +13 more
europepmc +2 more sources