Results 1 to 10 of about 1,700,295 (170)

Lasso-grafting of macrocyclic peptide pharmacophores yields multi-functional proteins

Nature Communications, 2021
RaPID (Random non-standard Peptides Integrated Discovery) enables discovery of small macrocyclic peptides binding desired targets. Here, the authors propose lasso-grafting: the RaPID-derived peptides are implanted onto diverse proteins and maintain both ...
Emiko Mihara, Satoshi Watanabe, Nasir K. Bashiruddin, Nozomi Nakamura, Kyoko Matoba, Yumi Sano, Rumit Maini, Yizhen Yin, Katsuya Sakai, Takao Arimori, Kunio Matsumoto, Hiroaki Suga, Junichi Takagi   +12 more
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Structural mechanism of laminin recognition by integrin

Nature Communications, 2021
Recognition of laminin by integrin receptors mediates epithelial cell adhesion to basement membrane. Here, the structures of the α6β1 integrin alone and in complex with three-chain laminin-511 fragment reveal the laminin-integrin interface in molecular ...
Takao Arimori, Naoyuki Miyazaki, Emiko Mihara, Mamoru Takizawa, Yukimasa Taniguchi, Carlos Cabañas, Kiyotoshi Sekiguchi, Junichi Takagi   +7 more
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Rapid measurement of heteronuclear transverse relaxation rates using non-uniformly sampled R_1ρ accordion experiments [PDF]

Magnetic Resonance, 2021
Multidimensional, heteronuclear NMR relaxation methods are used extensively to characterize the dynamics of biological macromolecules. Acquisition of relaxation datasets on proteins typically requires significant measurement time, often several days ...
S. Wernersson, G. Carlström, A. Jakobsson, M. Akke   +3 more
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Functional role of the Frizzled linker domain in the Wnt signaling pathway

Communications Biology, 2022
The linker domain of Frizzled receptors has a varying effect on the functional levels of canonical and non-canonical Wnt signaling, with FZD chimeras influencing the binding of Wnt proteins and the recruitment of proteins regulating downstream signaling.
Seung-Bum Ko, Emiko Mihara, Yedarm Park, Kyeonghwan Roh, Chanhee Kang, Junichi Takagi, Injin Bang, Hee-Jung Choi   +7 more
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Higher-Order Architecture of Cell Adhesion Mediated by Polymorphic Synaptic Adhesion Molecules Neurexin and Neuroligin

Cell Reports, 2012
Polymorphic adhesion molecules neurexin and neuroligin (NL) mediate asymmetric trans-synaptic adhesion, which is crucial for synapse development and function.
Hiroki Tanaka, Naoyuki Miyazaki, Kyoko Matoba, Terukazu Nogi, Kenji Iwasaki, Junichi Takagi   +5 more
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Active and water-soluble form of lipidated Wnt protein is maintained by a serum glycoprotein afamin/α-albumin

eLife, 2016
Wnt plays important role during development and in various diseases. Because Wnts are lipidated and highly hydrophobic, they can only be purified in the presence of detergents, limiting their use in various in vitro and in vivo assays.
Emiko Mihara, Hidenori Hirai, Hideki Yamamoto, Keiko Tamura-Kawakami, Mami Matano, Akira Kikuchi, Toshiro Sato, Junichi Takagi   +7 more
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FAK activity in cancer‐associated fibroblasts is a prognostic marker and a druggable key metastatic player in pancreatic cancer

EMBO Molecular Medicine, 2020
Cancer‐associated fibroblasts (CAFs) are considered the most abundant type of stromal cells in pancreatic ductal adenocarcinoma (PDAC), playing a critical role in tumour progression and chemoresistance; however, a druggable target on CAFs has not yet ...
Sonia Zaghdoudi, Emilie Decaup, Ismahane Belhabib, Rémi Samain, Stéphanie Cassant‐Sourdy, Julia Rochotte, Alexia Brunel, David Schlaepfer, Jérome Cros, Cindy Neuzillet, Manon Strehaiano, Amandine Alard, Richard Tomasini, Vinothini Rajeeve, Aurélie Perraud, Muriel Mathonnet, Oliver MT Pearce, Yvan Martineau, Stéphane Pyronnet, Corinne Bousquet, Christine Jean   +20 more
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Engineered ACE2 receptor therapy overcomes mutational escape of SARS-CoV-2

Nature Communications, 2021
Hoshino et al., engineer a human virus receptor, hACE2, and demonstrate its potential for overcoming SARS-CoV-2 mutations that otherwise hinder therapeutic interventions.
Yusuke Higuchi, Tatsuya Suzuki, Takao Arimori, Nariko Ikemura, Emiko Mihara, Yuhei Kirita, Eriko Ohgitani, Osam Mazda, Daisuke Motooka, Shota Nakamura, Yusuke Sakai, Yumi Itoh, Fuminori Sugihara, Yoshiharu Matsuura, Satoaki Matoba, Toru Okamoto, Junichi Takagi, Atsushi Hoshino   +17 more
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2D hybrid analysis: Approach for building three-dimensional atomic model by electron microscopy image matching

Scientific Reports, 2017
In this study, we develop an approach termed “2D hybrid analysis” for building atomic models by image matching from electron microscopy (EM) images of biological molecules.
Atsushi Matsumoto, Naoyuki Miyazaki, Junichi Takagi, Kenji Iwasaki   +3 more
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Biochemical aspects of bacterial strategies for handling the incomplete translation processes

Frontiers in Microbiology, 2014
During protein synthesis in cells, translating ribosomes may encounter abnormal situations that lead to retention of immature peptidyl-tRNA on the ribosome due to failure of suitable termination processes.
Yoshihiro eShimizu
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