Results 61 to 70 of about 4,765 (275)
SmallTalk: a novel small‐sized fusion tag for peptide expression and purification
FEBS Open Bio, EarlyView.The SmallTalk fusion tag allows for the efficient expression and purification of soluble recombinant proteins or peptides in Escherichia coli. Testing with SmallTalk‐GFP confirmed that the proteins were soluble and folded correctly, while SmallTalk‐Bin1b maintained its antimicrobial activity against various bacterial isolates. This streamlined workflow
Atika Tariq +3 more
wiley +1 more source
Effects of temperature on dormancy release in Norway spruce and Scots pine seedlings.
Silva Fennica, 1988 Models concerning the effects of temperature on dormancy release in woody plants were tested using two-year old seedlings of Scots pine ( L.) and Norway spruce ( (L.) H. Karst.). Chilling experiments suggest that the rest period has a distinct end point.
Hänninen, Heikki, Pelkonen, Paavo
doaj +1 more source
Phenological characterization, evaluation of chilling requirement and frost hardiness of almond (Prunuc dulcis Mill) [PDF]
, 2023 Belay Teweldemedhin Keleta +1 more
openalex +1 more source
FEBS Open Bio, EarlyView.Clinical analysis reveals significant dysregulation of FGFRL1 in esophageal cancer (EC) patients. RNAi‐coupled next‐generation sequencing (NGS) and in vitro study reveal FGFRL1‐mediated EC progression via EMT, PI3K/Akt, and Notch pathways. Functional assays confirm its role in tumor growth, migration, and invasion.
Aprajita Srivastava +3 more
wiley +1 more source
Evaluation of winter temperatures on apple budbreak using grafted twigs
Revista Brasileira de Fruticultura, 2013 Temperature is the main climate factor related to induction, maintenance and dormancy release in apple (Malus domestica Borkh.). The inadequate chilling exposure in apples causes budbreak problems, resulting in decrease in yield potential.
Fernando José Hawerroth +4 more
doaj +1 more source
Frontiers in Plant Science, 2020 The effects of winter chilling, spring forcing temperature, and photoperiod on spring phenology are well known for many European and North American species, but the environmental cues that regulate the spring phenology of East Asian species have not yet ...
Huanjiong Wang +3 more
doaj +1 more source
FEBS Open Bio, EarlyView.CRISPRI‐mediated gene silencing and phenotypic exploration in nontuberculous mycobacteria. In this Research Protocol, we describe approaches to control, monitor, and quantitatively assess CRISPRI‐mediated gene silencing in M. smegmatis and M. abscessus model organisms.
Vanessa Point +7 more
wiley +1 more source
FEBS Open Bio, EarlyView.NF90–NF45 functions as a negative regulator of methyltransferase‐like 3/14 (METTL3/14)‐mediated N6‐methyladenosine (m6A) modification on primary microRNAs (pri‐miRNAs). NF90–NF45 binds to anti‐oncogenic pri‐miRNAs and inhibits their m6A modification, thereby suppressing the biogenesis of anti‐oncogenic miRNAs.
Takuma Higuchi +6 more
wiley +1 more source
Influence of exogenous gibberellin (GA3) on fruit quality in different peach varieties (strains) [PDF]
Guoshu xuebao[Objective] Exogenous GA3 is widely used to regulate fruit growth and development, which is closely related to fruit quality. The sensitivity of exogenous GA3 varies among different fruit tree species. The aim of this study was to investigate the effects
CHEN Hong +6 more
doaj +1 more source
FEBS Open Bio, EarlyView.dUTPases are involved in balancing the appropriate nucleotide pools. We showed that dUTPase is essential for normal development in zebrafish. The different zebrafish genomes contain several single‐nucleotide variations (SNPs) of the dut gene. One of the dUTPase variants displayed drastically lower protein stability and catalytic efficiency as compared ...
Viktória Perey‐Simon +6 more
wiley +1 more source