Sorting of chromosomes by magnetic separation [PDF]
, 1988 Chromosomes were isolated from Chinese hamster x human hybrid cell lines containing four and nine human chromosomes. Human genomic DNA was biotinylated by nick translation and used to label the human chromosomes by in situ hybridization in suspension ...
A Oberteufer +30 more
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A method for nucleic acid hybridization to isolated chromosomes in suspension [PDF]
, 1987 A procedure was developed to provide differential fluorescent staining of metaphase chromosomes in suspension following nucleic acid hybridization. For this purpose metaphase chromosomes were isolated from a Chinese hamster x human hybrid cell line ...
Bier, Frank +5 more
core +1 more source
Generation of induced pluripotent stem cells from Chinese hamster embryonic fibroblasts
Stem Cell Research, 2017 We derived a stable cell line from Chinese hamster embryonic fibroblasts by transduction of four mouse transcription factors (M3O, Sox2, Klf4, and n-Myc) using a lentiviral vector.
Haiyun Pei +7 more
doaj +1 more source
Haematologica, 2018 Immune responses to factor VIII remain the greatest complication in the treatment of severe hemophilia A. Recent epidemiological evidence has highlighted that recombinant factor VIII produced in baby hamster kidney cells is more immunogenic than factor ...
Jesse D. Lai +7 more
doaj +1 more source
Evidence for the role of proteoglycans in cation-mediated gene transfer [PDF]
, 1996 We report evidence that gene complexes, consisting of polycations and plasmid DNA enter cells via binding to membrane-associated proteoglycans. Treatment of HeLa cells with sodium chlorate, a potent inhibitor of proteoglycan sulfation, reduced luciferase
Baldeschwieler, John D. +1 more
core +1 more source
A reference genome of the Chinese hamster based on a hybrid assembly strategy
Biotechnology and Bioengineering, 2018 Accurate and complete genome sequences are essential in biotechnology to facilitate genome‐based cell engineering efforts. The current genome assemblies for Cricetulus griseus, the Chinese hamster, are fragmented and replete with gap sequences and ...
O. Rupp +17 more
semanticscholar +1 more source
Immunocytochemical localization of chromatin regions UV-microirradiated in S phase or anaphase : Evidence for a territorial organization of chromosomes during cell cycle of cultured Chinese hamster cells [PDF]
, 1983 Chinese hamster cells (M3-1 line) in S phase were laser-UV-microirradiated (λ, 257 nm) at a small site of the nucleus. Cells were fixed either immediately thereafter or in subsequent stages of the cell cycle, including prophase and metaphase.
Avivi +33 more
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Enhancing the toolbox to study IL-17A in cattle and sheep
Veterinary Research, 2017 The development of methods to detect cytokine expression by T cell subsets in ruminants is fundamental to strategic development of new livestock vaccines for prevention of infectious diseases. It has been possible to detect T cell expression of IFN-γ, IL-
Sean R. Wattegedera +9 more
doaj +1 more source
Current Therapeutic Research, 2014 Objective: We aimed to assess the therapeutic efficacy of differentially modified soluble receptor for advanced glycation end products (sRAGE) in vivo using vessel ultrasound sonography and to compare the sonography data with those from postmortem ...
Hyun-Jin Tae, DVM, PhD +6 more
doaj +1 more source
UV micro-irradiation of the Chinese hamster cell nucleus and caffeine post-treatment immunocytochemical localization of DNA photolesions in cells with partial and generalized chromosome shattering [PDF]
, 1983 UV micro-irradiation of a small part of the Chinese hamster nucleus and caffeine post-incubation often results in shattered chromosomes at the first post-irradiation mitosis.
Cornelis +26 more
core +1 more source