Results 21 to 30 of about 130,827 (311)
Data in Brief, 2020 The data presented in this article relates to the manuscript entitled ‘Engineering of Chinese hamster ovary cell lipid metabolism results in an expanded ER and enhanced recombinant biotherapeutic protein production’, published in the Journal Metabolic ...
James D. Budge +11 more
doaj +1 more source
Enhancing the toolbox to study IL-17A in cattle and sheep
Veterinary Research, 2017 The development of methods to detect cytokine expression by T cell subsets in ruminants is fundamental to strategic development of new livestock vaccines for prevention of infectious diseases. It has been possible to detect T cell expression of IFN-γ, IL-
Sean R. Wattegedera +9 more
doaj +1 more source
Induction of chromosome damage by ultraviolet light and caffeine: Correlation of cytogenetic evaluation and flow karyotype [PDF]
, 1982 Asynchrononously growing cells of a M3-1 Chinese hamster line were ultraviolet (UV) irradiated ( = 254 nm) with UV fluences up to 7.5 J/m2. After irradiation, cells were incubated with or without 2 mM caffeine for 20 hr, then mitotic cells were selected ...
Carrano +20 more
core +1 more source
Current Therapeutic Research, 2014 Objective: We aimed to assess the therapeutic efficacy of differentially modified soluble receptor for advanced glycation end products (sRAGE) in vivo using vessel ultrasound sonography and to compare the sonography data with those from postmortem ...
Hyun-Jin Tae, DVM, PhD +6 more
doaj +1 more source
Micro-Raman spectroscopy of chromosomes [PDF]
, 1984 Raman spectra of intact chromosomes (Chinese hamster), recorded with a microspectrometer, are reported. The spectra could be assigned to protein and DNA contributions.
Albrecht +16 more
core +3 more sources
Elucidation of Proteoforms of Chinese Hamster Ovary (CHO) Phospholipase B-Like 2 (PLBL2) Captured From a Monoclonal Antibody. [PDF]
Biotechnol BioengA platform approach to the affinity capture and enrichment of host cell proteins to enable their subsequent deep characterization and elucidation of variants or proteoforms (e.g., molecular size variants, charge variants, glycoforms, etc.). ABSTRACT Removing host cell proteins (HCPs) during biotherapeutic manufacturing is essential to ensure patient ...
Dolan ME +6 more
europepmc +2 more sources
Novel cell lines derived from Chinese hamster kidney tissue.
PLoS ONE, 2022 Immortalized kidney cell lines are widely used in basic and applied research such as cell permeability tests and drug screening. Although many cell lines have been established from kidney tissues, the immortalization process has not been clarified in ...
Yoshinori Kawabe, Masamichi Kamihira
doaj +1 more source
Screening Strategies for High-Yield Chinese Hamster Ovary Cell Clones
Frontiers in Bioengineering and Biotechnology, 2022 Chinese hamster ovary (CHO) cells are by far the most commonly used mammalian expression system for recombinant expression of therapeutic proteins in the pharmaceutical industry.
Wenwen Yang +8 more
doaj +1 more source
Journal of Lipid Research, 1989 Three mammalian cell lines were examined for their ability to metabolize the regulatory oxysterol, 25-hydroxycholesterol, and derepress 3-hydroxy-3-methylglutaryl CoA reductase.
F R Taylor, A A Kandutsch
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Rabl's model of the interphase chromosome arrangement tested in Chinise hamster cells by premature chromosome condensation and laser-UV-microbeam experiments [PDF]
, 1982 In 1885 Carl Rabl published his theory on the internal structure of the interphase nucleus. We have tested two predictions of this theory in fibroblasts grown in vitro from a female Chinese hamster, namely (1) the Rabl-orientation of interphase ...
C Cremer +34 more
core +1 more source