Results 31 to 40 of about 130,827 (311)

Detection of laser-UV microirradiation-induced DNA photolesions by immunofluorescent staining [PDF]

, 1980
A low-power laser-UV microbeam of wave-length 257 nm was used for microirradiation of a small part of the nucleus of Chinese hamster cells. Following fixation in interphase or in the subsequent metaphase indirect immunofluorescent staining was performed ...
Cremer, Christoph, Cremer, Thomas, Fukada, M, Nakanishi, K.   +3 more
core   +1 more source

Induction of chromosome shattering by ultraviolet light and caffeine: The influence of different distributions of photolesions [PDF]

, 1986
Cells of synchonized and of asynchronously growing cultures of a V79 Chinese hamster line were microirradiated with a low poweer laser-UV-microbeam of wavelength 257 nm.
Barranco, Brenner, Cornelis, Cremer, Cremer, Cremer, Cremer, Cremer, Cremer, Cremer, Cremer, Cremer, Cremer, Cremer, Cremer, Cremer, Cremer, Cremer, González-Fernández, Hens, Kihlman, Kihlman, Kihlman, Kihlman, Lau, Lehmann, Moreno, Nilsson, Painter, Painter, Raith, Rappold, Roberts, Schardin, Sedgwick, Setlow, Stack, Steward, Tatsumi, Tobey, Tomulin, Zorn, Zorn   +42 more
core   +1 more source

Comprehensive Mapping of Functional Enhancers in Chinese Hamster Ovary Cells. [PDF]

Biotechnol Bioeng
In this study, the authors identified regions with enhancer activity in the CHO cell genome using genome‐wide and targeted STARR‐seq. The analysis revealed thousands of newly identified enhancer sequences, where some of the most active sequences could drive transgene expression at levels similar to or higher than strong viral enhancers.
Santos M, Johari YB, Biggins L, Elliott NC, Schoenfelder S, Boddireddy M, Fabian DK, Anbar M, O'Callaghan PM, Rugg-Gunn PJ.   +9 more
europepmc   +2 more sources

UV micro-irradiation of the Chinese hamster cell nucleus and caffeine post-treatment immunocytochemical localization of DNA photolesions in cells with partial and generalized chromosome shattering [PDF]

, 1983
UV micro-irradiation of a small part of the Chinese hamster nucleus and caffeine post-incubation often results in shattered chromosomes at the first post-irradiation mitosis.
Cornelis, Cornelis, Cornelis, Cremer, Cremer, Cremer, Cremer, Cremer, Cremer, Cremer, Cremer, Cremer, Cremer, Cremer, Cremer, Deaven, Fukuda, Gray, Hsu, Kihlman, Lehmann, Nilsson, Stack, Stubblefield, Taylor, Zorn, Zorn   +26 more
core   +1 more source

Analysis of chromosome positions in the interphase nucleus of Chinese hamster cells by laser-UV-microirradiation experiments [PDF]

, 1982
Unsynchronized cells of an essentially diploid strain of female Chinese hamster cells derived from lung tissue (CHL) were laser-UV-microirradiated (=257 nm) in the nucleus either at its central part or at its periphery.
B Dutrillaux, BA Kihlman, C Cremer, C Cremer, C Cremer, C Cremer, C Wollenberg, C Zorn, C Zorn, C. Cremer, DE Barton, DE Comings, DE Comings, E Therman, F Vogel, FE Fosse Del, H Kato, H. Baumann, HD Hager, JW Gray, K Church, K Nilsson, L Avivi, L Hens, L Hens, L. Hens, LL Deaven, M Ray, M Schmid, M. Kirsch-Volders, S Brenner, T Caspersson, T Cremer, T Cremer, T. Cremer, T. Schneider, Th Boveri, W Sauerbier, Y Moroi   +38 more
core   +1 more source

Laser-UV-microirradiation of interphase nuclei and posttreatment with caffeine: a new approach to establish the arrangement of interphase chromosomes [PDF]

, 1976
Laser UV microirradiation of Chinese hamster interphase cells combined with caffeine post-treatment produced different patterns of chromosome damage in mitosis following irradiation of a small area of the nucleus that may be classified in three ...
B. A. Kihlman, B. A. Kihlman, C. Cremer, C. Cremer, D. E. Comings, D. E. Comings, D. Warburton, F. Vogel, G. Moreno, H. G. Schwarzacher, H. J. Barr, K. Nilsson, K. Sperling, L. Hens, L. L. Deaven, M. Schmid, R. J. Singh, S. L. Schor, U. Nur, V. N. Sakharov   +19 more
core   +1 more source

Investigating the Glucagon Receptor and Glucagon-Like Peptide 1 Receptor Activity of Oxyntomodulin-Like Analogues in Male Wistar Rats

Current Therapeutic Research, 2015
Aims: To investigate the effect of Glu-3 OXM-like analogues on food intake and bodyweight in male rats. Background: Oxyntomodulin (OXM) is a natural agonist at both the glucagon receptor (GCGr) and the glucagon-like peptide 1 receptor (GLP-1r), and ...
Samantha L. Price, MRes, James S. Minnion, PhD, Stephen R. Bloom, MD   +2 more
doaj   +1 more source

Model-based analysis of N-glycosylation in Chinese hamster ovary cells. [PDF]

PLoS ONE, 2017
The Chinese hamster ovary (CHO) cell is the gold standard for manufacturing of glycosylated recombinant proteins for production of biotherapeutics. The similarity of its glycosylation patterns to the human versions enable the products of this cell line ...
Frederick J Krambeck, Sandra V Bennun, Mikael R Andersen, Michael J Betenbaugh   +3 more
doaj   +1 more source

Preclinical development of a molecular clamp‐stabilised subunit vaccine for severe acute respiratory syndrome coronavirus 2

Clinical & Translational Immunology, 2021
Objectives Efforts to develop and deploy effective vaccines against severe acute respiratory syndrome coronavirus 2 (SARS‐CoV‐2) continue at pace. Here, we describe rational antigen design through to manufacturability and vaccine efficacy of a prefusion ...
Daniel Watterson, Danushka K Wijesundara, Naphak Modhiran, Francesca L Mordant, Zheyi Li, Michael S Avumegah, Christopher LD McMillan, Julia Lackenby, Kate Guilfoyle, Geert vanAmerongen, Koert Stittelaar, Stacey TM Cheung, Summa Bibby, Mallory Daleris, Kym Hoger, Marianne Gillard, Eve Radunz, Martina L Jones, Karen Hughes, Ben Hughes, Justin Goh, David Edwards, Judith Scoble, Lesley Pearce, Lukasz Kowalczyk, Tram Phan, Mylinh La, Louis Lu, Tam Pham, Qi Zhou, David A Brockman, Sherry J Morgan, Cora Lau, Mai H Tran, Peter Tapley, Fernando Villalón‐Letelier, James Barnes, Andrew Young, Noushin Jaberolansar, Connor AP Scott, Ariel Isaacs, Alberto A Amarilla, Alexander A Khromykh, Judith MA van denBrand, Patrick C Reading, Charani Ranasinghe, Kanta Subbarao, Trent P Munro, Paul R Young, Keith J Chappell   +49 more
doaj   +1 more source

His+ reversions Caused in Salmonella typhimurium by different types of ionizing radiation [PDF]

, 1988
The yield of his+ reversions in the Ames Salmonella tester strain TA2638 has been determined for 60Co γ rays, 140 kV X rays, 5.4 keV characteristic X rays, 2.2 MeV protons, 3.1 MeV α particles, and 18 MeV/U Fe ions.
Fitzek, M., Kellerer, Albrecht M., Roos, H., Thomas, W.-H.   +3 more
core   +1 more source