Results 31 to 40 of about 131,127 (291)

Screening Strategies for High-Yield Chinese Hamster Ovary Cell Clones

Frontiers in Bioengineering and Biotechnology, 2022
Chinese hamster ovary (CHO) cells are by far the most commonly used mammalian expression system for recombinant expression of therapeutic proteins in the pharmaceutical industry.
Wenwen Yang, Wenwen Yang, Junhe Zhang, Junhe Zhang, Junhe Zhang, Yunxi Xiao, Wenqing Li, Tianyun Wang, Tianyun Wang   +8 more
doaj   +1 more source

Induction of chromosome shattering by ultraviolet light and caffeine: The influence of different distributions of photolesions [PDF]

, 1986
Cells of synchonized and of asynchronously growing cultures of a V79 Chinese hamster line were microirradiated with a low poweer laser-UV-microbeam of wavelength 257 nm.
Barranco, Brenner, Cornelis, Cremer, Cremer, Cremer, Cremer, Cremer, Cremer, Cremer, Cremer, Cremer, Cremer, Cremer, Cremer, Cremer, Cremer, Cremer, González-Fernández, Hens, Kihlman, Kihlman, Kihlman, Kihlman, Lau, Lehmann, Moreno, Nilsson, Painter, Painter, Raith, Rappold, Roberts, Schardin, Sedgwick, Setlow, Stack, Steward, Tatsumi, Tobey, Tomulin, Zorn, Zorn   +42 more
core   +1 more source

Metabolism of 25-hydroxycholesterol in mammalian cell cultures. Side-chain scission to pregnenolone in mouse L929 fibroblasts.

Journal of Lipid Research, 1989
Three mammalian cell lines were examined for their ability to metabolize the regulatory oxysterol, 25-hydroxycholesterol, and derepress 3-hydroxy-3-methylglutaryl CoA reductase.
F R Taylor, A A Kandutsch
doaj   +1 more source

Novel cell lines derived from Chinese hamster kidney tissue.

PLoS ONE, 2022
Immortalized kidney cell lines are widely used in basic and applied research such as cell permeability tests and drug screening. Although many cell lines have been established from kidney tissues, the immortalization process has not been clarified in ...
Yoshinori Kawabe, Masamichi Kamihira
doaj   +1 more source

Induction of chromosome damage by ultraviolet light and caffeine: Correlation of cytogenetic evaluation and flow karyotype [PDF]

, 1982
Asynchrononously growing cells of a M3-1 Chinese hamster line were ultraviolet (UV) irradiated ( = 254 nm) with UV fluences up to 7.5 J/m2. After irradiation, cells were incubated with or without 2 mM caffeine for 20 hr, then mitotic cells were selected ...
Carrano, Carrano, Carrano, Chu, Cremer, Cremer, Cremer, Cremer, Gray, Gray, Gray, Horan, Kato, Kihlman, Langlois, Lovelace, Moore, Nilsson, Otto, Roberts, Yu   +20 more
core   +1 more source

Analysis of chromosome positions in the interphase nucleus of Chinese hamster cells by laser-UV-microirradiation experiments [PDF]

, 1982
Unsynchronized cells of an essentially diploid strain of female Chinese hamster cells derived from lung tissue (CHL) were laser-UV-microirradiated (=257 nm) in the nucleus either at its central part or at its periphery.
B Dutrillaux, BA Kihlman, C Cremer, C Cremer, C Cremer, C Cremer, C Wollenberg, C Zorn, C Zorn, C. Cremer, DE Barton, DE Comings, DE Comings, E Therman, F Vogel, FE Fosse Del, H Kato, H. Baumann, HD Hager, JW Gray, K Church, K Nilsson, L Avivi, L Hens, L Hens, L. Hens, LL Deaven, M Ray, M Schmid, M. Kirsch-Volders, S Brenner, T Caspersson, T Cremer, T Cremer, T. Cremer, T. Schneider, Th Boveri, W Sauerbier, Y Moroi   +38 more
core   +1 more source

Detection of laser-UV microirradiation-induced DNA photolesions by immunofluorescent staining [PDF]

, 1980
A low-power laser-UV microbeam of wave-length 257 nm was used for microirradiation of a small part of the nucleus of Chinese hamster cells. Following fixation in interphase or in the subsequent metaphase indirect immunofluorescent staining was performed ...
Cremer, Christoph, Cremer, Thomas, Fukada, M, Nakanishi, K.   +3 more
core   +1 more source

Laser-UV-microirradiation of interphase nuclei and posttreatment with caffeine: a new approach to establish the arrangement of interphase chromosomes [PDF]

, 1976
Laser UV microirradiation of Chinese hamster interphase cells combined with caffeine post-treatment produced different patterns of chromosome damage in mitosis following irradiation of a small area of the nucleus that may be classified in three ...
B. A. Kihlman, B. A. Kihlman, C. Cremer, C. Cremer, D. E. Comings, D. E. Comings, D. Warburton, F. Vogel, G. Moreno, H. G. Schwarzacher, H. J. Barr, K. Nilsson, K. Sperling, L. Hens, L. L. Deaven, M. Schmid, R. J. Singh, S. L. Schor, U. Nur, V. N. Sakharov   +19 more
core   +1 more source

Elucidation of Proteoforms of Chinese Hamster Ovary (CHO) Phospholipase B-Like 2 (PLBL2) Captured From a Monoclonal Antibody. [PDF]

Biotechnol Bioeng
A platform approach to the affinity capture and enrichment of host cell proteins to enable their subsequent deep characterization and elucidation of variants or proteoforms (e.g., molecular size variants, charge variants, glycoforms, etc.). ABSTRACT Removing host cell proteins (HCPs) during biotherapeutic manufacturing is essential to ensure patient ...
Dolan ME, Wang LL, Tedeschi A, Wang Y, Barton C, Oppenheim SF, Zhou ZS.   +6 more
europepmc   +2 more sources

His+ reversions Caused in Salmonella typhimurium by different types of ionizing radiation [PDF]

, 1988
The yield of his+ reversions in the Ames Salmonella tester strain TA2638 has been determined for 60Co γ rays, 140 kV X rays, 5.4 keV characteristic X rays, 2.2 MeV protons, 3.1 MeV α particles, and 18 MeV/U Fe ions.
Fitzek, M., Kellerer, Albrecht M., Roos, H., Thomas, W.-H.   +3 more
core   +1 more source