Results 81 to 90 of about 10,635 (98)
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Lysosomes in Chinese Hamster fibroblasts in culture
Experimental Cell Research, 1964Abstract A modified Gomori method for staining cultured cells for acid phosphatase is described, which permits a chosen cell to be photographed after various times of incubation in the substrate mixture, and thereby assists in the identification of artefacts.
J.T. Dingle, M.R. Daniel, T.R. Munro
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Pachytene mapping of the male Chinese hamster
Cytogenetic and Genome Research, 1976Minced seminiferous tubules of male Chinese hamsters, when treated with a mixture of trypsin (one part) and McCoy’s 5a growth medium with 20 % fetal calf serum (nine parts) at 4° C, washed twice with the regular growth medium, incubated at 37° C in growth medium for 4 h, and harvested without Colcemid and hypotonic pretreatments, gave excellent ...
Amara Markvong, T. C. Hsu, S. Pathak
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Architecture of the Chinese hamster metaphase chromosome
Chromosoma, 1971The development of procedures for the isolation of unfixed metaphase chromosomes has made feasible a direct analysis of their morphology. Wholemount stereo electron microscopy was used to examine intact and partially disrupted chromosomes produced by physical shearing and extraction with salt and urea solutions.
Wayne Wray, Elton Stubblefield
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Chinese hamster ovary chromosomes and antigens in tobacco/hamster heterokaryons
Journal of Heredity, 1981Tobacco GGLL/hamster CHO hamster heterokaryon frequency following cell fusion has been raised to 4-10 percent with a modified PEG procedure. In heterokaryons, GGLL nuclei become activated, adhere to and appear to fuse with CHO nucleo. Metaphase CHO chromosomes can be identified 24 hours after fusion and immunofluorescent CHO cell surface antigens ...
Iris A. Mastrangelo, J. Mitra
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Endocytosis in Chinese hamster fibroblasts
Experimental Cell Research, 1973Abstract Endocytosis in Chinese hamster ovary fibroblasts was investigated by measuring the rate of uptake of 3 H-sucrose, which is known to enter cells only by endocytosis. Serum, polyvinylpyrrolidone (PVP), adenosine triphosphate, insulin, and cyclic 3′,5′-adenosine monophosphate, all of which are known to increase the rate of endocytosis by other
G. Becker, M.J. Ashwood-Smith
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Toxicity of 90 Sr- 90 Y in Chinese Hamsters
Radiation Research, 1974Strontium-90 citrate was injected intraperitoneally into Chinese hamsters at activity levels of 0.2, 0.5, 1.0, 2.0, 3.0, and 5.0 μCi/g body weight and the animals held for lifespan observation. Additional hamsters injected with sodium citrate served as controls.
Antone L. Brooks +2 more
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Mutation Research/Fundamental and Molecular Mechanisms of Mutagenesis, 1975
Mutant Chinese hamster ovarian (CHO) cells with a resistance to 7-10(-7) and 8-10(-7) M cycloheximide (CHM) were induced at mutation rates of 1.9-5.2-10(-3) and 1.6-1.8-10(-3) respectively after treatment with N-nitrosomethylurea (NMU) at 100 mug/ml. The induced mutation rates differed by two orders of magnitude from the spontaneous rate of mutation to
Nina B. Varshaver +3 more
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Mutant Chinese hamster ovarian (CHO) cells with a resistance to 7-10(-7) and 8-10(-7) M cycloheximide (CHM) were induced at mutation rates of 1.9-5.2-10(-3) and 1.6-1.8-10(-3) respectively after treatment with N-nitrosomethylurea (NMU) at 100 mug/ml. The induced mutation rates differed by two orders of magnitude from the spontaneous rate of mutation to
Nina B. Varshaver +3 more
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Effect of Distamycin A on Chinese hamster chromosomes
Experimental Cell Research, 1977G. Prantera +3 more
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Metabolomics of Chinese Hamster Ovary Cells
Increasing demand of protein biotherapeutics produced using Chinese hamster ovary (CHO) cell lines necessitates improvement in the production yield of the bioprocess. Various cell engineering, improved media formulation and process-design based approaches utilizing the power of OMICS technologies, specifically, genomics and proteomics, have been ...Rita, Singh +5 more
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[73] Chinese hamster purine nucleoside phosphorylase
1978Publisher Summary This chapter describes the purification procedure of nucleoside phosphorylase enzyme from Chinese hamster V79 tissue culture cells. Purine nucleoside phosphorylase (purine nucleoside: orthophosphate ribosyltransferase) in eukaryotes catalyzes the reversible conversion among the purine bases, hypoxanthine and guanine, and their ...
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