Results 91 to 100 of about 110,281 (284)

Signaling across the Platelet Adhesion Receptor Glycoprotein Ib-IX Induces αIIbβ3 Activation Both in Platelets and a Transfected Chinese Hamster Ovary Cell System*

Journal of Biological Chemistry, 2000
In platelets, αIIbβ3 exists in a form that cannot bind adhesive proteins in the plasma; although it can interact with immobilized fibrinogen it cannot interact with immobilized von Willebrand factor in the vessel wall.
Y. Zaffran, Sylvie C. Meyer, E. Negrescu, K. Reddy, J. Fox   +4 more
semanticscholar   +1 more source

Innovative methodology for flexible monitoring of various bioprocesses by using pre‐existing Raman data coupled with automated transfer learning technique

Biotechnology Progress, EarlyView.
The application of the transfer learning algorithm successfully allowed the use of a model built on process A for real‐time monitoring of process B. This study shows the improvement obtained with this methodology compared to the direct application of the model.
Adèle Schini, Hadi El Radi, Johan Cailletaud, Célia Sanchez, Nathan Gay, Bruno Ebel, Emmanuel Guedon, Laurent Jourdainne   +7 more
wiley   +1 more source

Thrombomodulin is essential for recombinant Fc-fusion protein production in Chinese hamster ovary cells via multiple signaling pathways

Journal of Biological Engineering
Background Chinese hamster ovary (CHO) cells are the predominant cell line used for biotherapeutic production. To reduce the cost of therapeutic recombinant proteins produced in CHO cells, efforts have been made over decades to improve overall yield ...
Xiaonan Ma, Nawei Wang, Qihang Yan, Shuang Li, Guorong Hu, Lunxiao Zhao, Ruoyin Xiao, Mingqi Wang, Ningning Ma   +8 more
doaj   +1 more source

Protocol for microfluidic single-cell cultivation and live-cell imaging of Chinese hamster ovary suspension cell lines

STAR Protocols
Summary: Microfluidic single-cell cultivation (MSCC) is a powerful tool for investigating the cellular behavior of various cell types at the single-cell level.
Julian Schmitz, Boris Yermakov, Alexander Grünberger   +2 more
doaj   +1 more source

Growth of Pseudotypes of Vesicular Stomatitis Virus with N-Tropic Murine Leukemia Virus Coats in Cells Resistant to N-Tropic Viruses [PDF]

, 1973
Formation of pseudotypes between murine RNA tumor viruses and vesicular stomatitis virus (VSV) has been confirmed. Pseudotypes of VSV genomes coated by the surface envelope from an N-tropic tumor virus grew equally well in cells homozygous for either the
Baltimore, David, Besmer, Peter, Chu, Louise, Huang, Alice S.   +3 more
core   +1 more source

Characterizing steady states of genome-scale metabolic networks in continuous cell cultures

, 2017
We present a model for continuous cell culture coupling intra-cellular metabolism to extracellular variables describing the state of the bioreactor, taking into account the growth capacity of the cell and the impact of toxic byproduct accumulation.
Fernandez-de-Cossio-Diaz, Jorge, León, Kalet, Mulet, Roberto   +2 more
core   +3 more sources

Engineering novel complement activity into a pulmonary surfactant protein [PDF]

, 2010
Complement neutralizes invading pathogens, stimulates inflammatory and adaptive immune responses, and targets non- or altered-self structures for clearance.
Anthony H. Keeble, Brodsky-Doyle, Chen, Chen, Christopher Furze, Daniel A. Mitchell, Doliana, Emsley, Fan, Feinberg, Girija, Haurum, Heise, Hohenester, Hoppe, Jensenius, Julia Toth, Kaufman, Kurata, Phillips, Porter, Reid, Reid, Reid, Russell Wallis, Scherer, Shoulders, Sim, Strang, Teillet, Uemura, Umakhanth Venkatraman Girija, Wallis, Wallis, Wallis, Wallis, Wallis, Wallis, Wilhelm J. Schwaeble   +38 more
core   +2 more sources

Chemical engineering as an essential element of industrial biotechnology in Mexico: New aims in research and university education

The Canadian Journal of Chemical Engineering, EarlyView.
Abstract On the centennial of higher education in Chemical Engineering in Mexico, it is pertinent to revisit the key stages that have contributed to its consolidation as a vital discipline for the nation's scientific and technological advancement. Although the initial mission of chemical engineering education was primarily oriented toward the training ...
Agustín López Munguía, Octavio T. Ramírez, Rafael Vazquez‐Duhalt, Eduardo Bárzana   +3 more
wiley   +1 more source

Cell Propagation of Cholera Toxin CTA ADP-Ribosylating Factor by Exosome Mediated Transfer [PDF]

, 2018
In this study, we report how the cholera toxin (CT) A subunit (CTA), the enzyme moiety responsible for signaling alteration in host cells, enters the exosomal pathway, secretes extracellularly, transmits itself to a cell population.
Boussadia, Zaira, Carollo, Maria, Fabbri, Alessia, Falchi, Mario, Fecchi, Katia, Fiani, Maria Luisa, Gallina, Angelo, Palermo, Angela, Parisi, Sofia, Pasquini, Luca, Sargiacomo, Massimo, Zanetti, Cristiana   +11 more
core   +1 more source

The In Vitro Transgenic Rodent Assay in Primary MutaMouse Hepatocytes Compared to the Mammalian Cell Gene Mutation Assay Using the HPRT Gene

Environmental and Molecular Mutagenesis, EarlyView.
ABSTRACT Gene mutations can be detected in mammalian cells in vitro using indicator genes such as the hypoxanthine‐guanine‐phosphoribosyltransferase (HPRT) gene. These assays have been adopted as OECD test guidelines (TG, e.g., OECD TG no. 476) and are used for regulatory purposes.
Alina Göpfert, Kylee Kendra Marie Ronnenberg, Claudia Ruelker, Silke Spang, Naveed Honarvar, Robert Landsiedel   +5 more
wiley   +1 more source