Results 11 to 20 of about 128,450 (211)

Generation of induced pluripotent stem cells from Chinese hamster embryonic fibroblasts

Stem Cell Research, 2017
We derived a stable cell line from Chinese hamster embryonic fibroblasts by transduction of four mouse transcription factors (M3O, Sox2, Klf4, and n-Myc) using a lentiviral vector.
Haiyun Pei, Hsu-Yuan Fu, Hiroyuki Hirai, Dong Seong Cho, Timothy D. O'Brien, James Dutton, Catherine M. Verfaillie, Wei-Shou Hu   +7 more
doaj   +1 more source

N-linked glycosylation modulates the immunogenicity of recombinant human factor VIII in hemophilia A mice

Haematologica, 2018
Immune responses to factor VIII remain the greatest complication in the treatment of severe hemophilia A. Recent epidemiological evidence has highlighted that recombinant factor VIII produced in baby hamster kidney cells is more immunogenic than factor ...
Jesse D. Lai, Laura L. Swystun, Dominique Cartier, Kate Nesbitt, Cunjie Zhang, Christine Hough, James W. Dennis, David Lillicrap   +7 more
doaj   +1 more source

Genomic landscapes of Chinese hamster ovary cell lines as revealed by the Cricetulus griseus draft genome

Nature Biotechnology, 2013
Genome sequences of the Chinese hamster and six Chinese hamster ovary cell lines provide a resource to aid in improving production of recombinant proteins.
N. Lewis, Xin Liu, Yuxiang Li, Harish Nagarajan, George Yerganian, Edward J. O'Brien, A. Bordbar, A. M. Roth, J. Rosenbloom, Chao Bian, Min Xie, Wenbin Chen, Ning Li, Deniz Baycin-Hizal, Haythem Latif, Jochen Forster, M. Betenbaugh, Iman Famili, Xun Xu, Jun Wang, B. Palsson   +20 more
semanticscholar   +1 more source

Vessel Ultrasound Sonographic Assessment of Soluble Receptor for Advanced Glycation End Products Efficacy in a Rat Balloon Injury Model

Current Therapeutic Research, 2014
Objective: We aimed to assess the therapeutic efficacy of differentially modified soluble receptor for advanced glycation end products (sRAGE) in vivo using vessel ultrasound sonography and to compare the sonography data with those from postmortem ...
Hyun-Jin Tae, DVM, PhD, Natalia Petrashevskaya, PhD, Ismayil Ahmet, PhD, Sungha Park, MD, PhD, Mark I. Talan, MD, PhD, Edward G. Lakatta, MD, Li Lin, PhD   +6 more
doaj   +1 more source

Advances in Escherichia coli-Based Therapeutic Protein Expression: Mammalian Conversion, Continuous Manufacturing, and Cell-Free Production

Biologics, 2023
Therapeutic proteins treat many acute and chronic diseases that were until recently considered untreatable. However, their high development cost keeps them out of reach of most patients around the world. One plausible solution to lower-cost manufacturing
Sarfaraz K. Niazi, Matthias Magoola
doaj   +1 more source

Antibody mix-and-read assays based on fluorescence intensity probes

mAbs, 2021
Antibodies and Fc fusion proteins are a rapidly growing class of pharmaceuticals. Cell culture and purification process development and operation require frequent measurement of product concentrations, commonly by complex enzyme-linked immunosorbent ...
Ujwal Patil, Atul Goyal, Binh Vu, Yanyun Liu, Vijay Maranholkar, Katerina Kourentzi, James M. Briggs, Richard C. Willson   +7 more
doaj   +1 more source

Lingering Dynamics in Microvascular Blood Flow [PDF]

Biophysical Journal 120(3) (2021), 432-439, 2021
The microvascular networks in the body of vertebrates consist of the smallest vessels such as arterioles, capillaries, and venules. The flow of RBCs through these networks ensures the gas exchange in as well as the transport of nutrients to the tissues. Any alterations in this blood flow may have severe implications on the health state.
arxiv   +1 more source

Stoichiometry of the Glial Glutamate Transporter GLT-1 Expressed Inducibly in a Chinese Hamster Ovary Cell Line Selected for Low Endogenous Na+-Dependent Glutamate Uptake

Journal of Neuroscience, 1998
Glutamate transport across the plasma membrane of neurons and glia is powered by the transmembrane electrochemical gradients for sodium, potassium, and pH, but there is controversy over the number of Na+ cotransported with glutamate. The stoichiometry of
L. Levy, O. Warr, D. Attwell
semanticscholar   +1 more source

THE EFFECTS OF ELECTRICAL STIMULATION AND LASER RADIATION ON F ACTIN REORGANIZATION IN A549 AND CHO AA8 CELL LINES

International Journal of Physiotherapy, 2015
Background: The aim of this report was to determine the effects of electrical stimulation and laser radiation on non-small lung cancer (A549) and Chinese Hamster Ovary cell line (CHO AA8).
Maciej Gagat, Magdalena Izdebska, Marta Hałas-Wiśniewska, Monika Struensee, Marcin Struensee, Anna Klimaszewska-Wiśniewska, Alina Grzanka   +6 more
doaj   +1 more source

A bioinformatics pipeline for the identification of CHO cell differential gene expression from RNA-Seq data [PDF]

Methods Mol Biol. 2017;1603:169-186, 2019
In recent years the publication of genome sequences for the Chinese hamster and Chinese hamster ovary (CHO) cell lines have facilitated study of these biopharmaceutical cell factories with unprecedented resolution. Our understanding of the CHO cell transcriptome, in particular, has rapidly advanced through the application of next-generation sequencing (
arxiv   +1 more source