Results 11 to 20 of about 110,193 (198)

Evidence for the role of proteoglycans in cation-mediated gene transfer [PDF]

open access: yes, 1996
We report evidence that gene complexes, consisting of polycations and plasmid DNA enter cells via binding to membrane-associated proteoglycans. Treatment of HeLa cells with sodium chlorate, a potent inhibitor of proteoglycan sulfation, reduced luciferase
Baldeschwieler, John D.   +1 more
core   +1 more source

Enhancing the toolbox to study IL-17A in cattle and sheep

open access: yesVeterinary Research, 2017
The development of methods to detect cytokine expression by T cell subsets in ruminants is fundamental to strategic development of new livestock vaccines for prevention of infectious diseases. It has been possible to detect T cell expression of IFN-γ, IL-
Sean R. Wattegedera   +9 more
doaj   +1 more source

Genotoxicity and Cytotoxicity Exerted by Pesticides in Different Biotic Matrices-An Overview of More Than a Decade of Experimental Evaluation [PDF]

open access: yes, 2014
Agrochemicals represent one of the most important sources of environmental pollution. Although attempts to reduce agrochemical use through organic agricultural practices and the use of other technologies to control pests continue, the problem is still
Larramendy, Marcelo Luis   +3 more
core   +2 more sources

N-linked glycosylation modulates the immunogenicity of recombinant human factor VIII in hemophilia A mice

open access: yesHaematologica, 2018
Immune responses to factor VIII remain the greatest complication in the treatment of severe hemophilia A. Recent epidemiological evidence has highlighted that recombinant factor VIII produced in baby hamster kidney cells is more immunogenic than factor ...
Jesse D. Lai   +7 more
doaj   +1 more source

A theoretical estimate for nucleotide sugar demand towards Chinese Hamster Ovary cellular glycosylation [PDF]

open access: yes, 2016
Glycosylation greatly influences the safety and efficacy of many of the highest-selling recombinant therapeutic proteins (rTPs). In order to define optimal cell culture feeding strategies that control rTP glycosylation, it is necessary to know how ...
Jimenez del Val, I   +2 more
core   +1 more source

A Metabolomics Approach to Increasing Chinese Hamster Ovary (CHO) Cell Productivity

open access: yesMetabolites, 2021
Much progress has been made in improving the viable cell density of bioreactor cultures in monoclonal antibody production from Chinese hamster ovary (CHO) cells; however, specific productivity (qP) has not been increased to the same degree. In this work,
Grace Yao   +5 more
doaj   +1 more source

Vessel Ultrasound Sonographic Assessment of Soluble Receptor for Advanced Glycation End Products Efficacy in a Rat Balloon Injury Model

open access: yesCurrent Therapeutic Research, 2014
Objective: We aimed to assess the therapeutic efficacy of differentially modified soluble receptor for advanced glycation end products (sRAGE) in vivo using vessel ultrasound sonography and to compare the sonography data with those from postmortem ...
Hyun-Jin Tae, DVM, PhD   +6 more
doaj   +1 more source

Metabolism of 25-hydroxycholesterol in mammalian cell cultures. Side-chain scission to pregnenolone in mouse L929 fibroblasts.

open access: yesJournal of Lipid Research, 1989
Three mammalian cell lines were examined for their ability to metabolize the regulatory oxysterol, 25-hydroxycholesterol, and derepress 3-hydroxy-3-methylglutaryl CoA reductase.
F R Taylor, A A Kandutsch
doaj   +1 more source

A point mutation of integrin beta 1 subunit blocks binding of alpha 5 beta 1 to fibronectin and invasin but not recruitment to adhesion plaques. [PDF]

open access: yes, 1992
A point mutation in a highly conserved region of the beta 1 subunit, Asp130 to Ala (D130A) substitution, abrogates the Arg-Gly-Asp (RGD)-dependent binding of alpha 5 beta 1 to fibronectin (FN) without disrupting gross structure or heterodimer assembly ...
Ginsberg, MH   +4 more
core   +2 more sources

Antibody mix-and-read assays based on fluorescence intensity probes

open access: yesmAbs, 2021
Antibodies and Fc fusion proteins are a rapidly growing class of pharmaceuticals. Cell culture and purification process development and operation require frequent measurement of product concentrations, commonly by complex enzyme-linked immunosorbent ...
Ujwal Patil   +7 more
doaj   +1 more source

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