Results 11 to 20 of about 110,281 (284)

Screening Strategies for High-Yield Chinese Hamster Ovary Cell Clones [PDF]

Frontiers in Bioengineering and Biotechnology, 2022
Chinese hamster ovary (CHO) cells are by far the most commonly used mammalian expression system for recombinant expression of therapeutic proteins in the pharmaceutical industry.
Wenwen Yang, Wenwen Yang, Junhe Zhang, Junhe Zhang, Junhe Zhang, Yunxi Xiao, Wenqing Li, Tianyun Wang, Tianyun Wang   +8 more
doaj   +2 more sources

Genomic landscapes of Chinese hamster ovary cell lines as revealed by the Cricetulus griseus draft genome. [PDF]

Nature Biotechnology, 2013
Genome sequences of the Chinese hamster and six Chinese hamster ovary cell lines provide a resource to aid in improving production of recombinant proteins.
Baycin-Hizal, Deniz, Betenbaugh, Michael, Bian, Chao, Bordbar, Aarash, Chen, Wenbin, Famili, Iman, Förster, Jochen, Latif, Haythem, Lewis, Nathan E, Li, Ning, Li, Yuxiang, Liu, Xin, Nagarajan, Harish, O'Brien, Edward, Palsson, Bernhard O, Rosenbloom, Jeffrey, Roth, Anne M, Wang, Jun, Xie, Min, Xu, Xun, Yerganian, George   +20 more
core   +3 more sources

4-(2,5-Dimethyl-1H-pyrrol-1-yl)-N-(2,5-dioxopyrrolidin-1-yl) benzamide improves monoclonal antibody production in a Chinese hamster ovary cell culture. [PDF]

PLoS ONE, 2021
There is a continuous demand to improve monoclonal antibody production for medication supply and medical cost reduction. For over 20 years, recombinant Chinese hamster ovary cells have been used as a host in monoclonal antibody production due to ...
Yuichi Aki, Yuta Katsumata, Hirofumi Kakihara, Koichi Nonaka, Kenshu Fujiwara   +4 more
doaj   +2 more sources

Chinese hamster ovary cell line DXB-11: chromosomal instability and karyotype heterogeneity [PDF]

Molecular Cytogenetics, 2021
Background Chinese hamster ovary cell lines, also known as CHO cells, represent a large family of related, yet quite different, cell lines which are metabolic mutants derived from the original cell line, CHO-ori.
Victoria I. Turilova, Tatyana S. Goryachaya, Tatiana K. Yakovleva   +2 more
doaj   +2 more sources

Genetic drivers of Chinese hamster ovary cell proliferation revealed by functional genomics [PDF]

iScience
Summary: Cell lines used in biopharmaceutical manufacturing are engineered to optimize protein yield, quality, and production speed. Cellular growth rate is a key bottleneck in the development of biopharmaceutical production cell lines.
Jannis Marzluf, Merle Rattay, Maximilian Goertz, Christoph Zehe, Ann-Cathrin Leroux   +4 more
doaj   +2 more sources

Comparative omics profiling reveals differences in biomass, energy production, and vesicle transport between CHO and fast-growing CHL-YN cells [PDF]

Scientific Reports
Chinese hamster lung (CHL)-YN cells are promising novel hosts for producing therapeutic antibodies with the potential to shorten the research, development, and manufacturing timelines in biopharmaceutical production.
Yu Tsunoda, Rintaro Arishima, Tatiana Boronina, Robert Cole, Noriko Yamano-Adachi, Michael Betenbaugh, Takeshi Omasa   +6 more
doaj   +2 more sources

CRISPR Technologies in Chinese Hamster Ovary Cell Line Engineering. [PDF]

Int J Mol Sci, 2023
The Chinese hamster ovary (CHO) cell line is a well-established platform for the production of biopharmaceuticals due to its ability to express complex therapeutic proteins with human-like glycopatterns in high amounts. The advent of CRISPR technology has opened up new avenues for the engineering of CHO cell lines for improved protein production and ...
Glinšek K, Bozovičar K, Bratkovič T.
europepmc   +3 more sources

Glycoengineering Chinese hamster ovary cells: a short history [PDF]

Biochemical Society Transactions, 2021
Biotherapeutic glycoproteins have revolutionised the field of pharmaceuticals, with new discoveries and continuous improvements underpinning the rapid growth of this industry. N-glycosylation is a critical quality attribute of biotherapeutic glycoproteins that influences the efficacy, half-life and immunogenicity of these drugs.
Roberto Donini, Stuart M. Haslam, Cleo Kontoravdi   +2 more
openaire   +4 more sources

Rapid Antibody Glycoengineering in Chinese Hamster Ovary Cells

Journal of Visualized Experiments, 2022
Recombinant monoclonal antibodies bind specific molecular targets and, subsequently, induce an immune response or inhibit the binding of other ligands. However, monoclonal antibody functionality and half-life may be reduced by the type and distribution of host-specific glycosylation. Attempts to produce superior antibodies have inspired the development
Marbiah, M, Kotidis, P, Donini, R, Gómez, IA, Jimenez Del Val, I, Haslam, SM, Polizzi, KM, Kontoravdi, C   +7 more
openaire   +4 more sources

Proteomic Analysis of Chinese Hamster Ovary Cells [PDF]

Journal of Proteome Research, 2012
To complement the recent genomic sequencing of Chinese hamster ovary (CHO) cells, proteomic analysis was performed on CHO cells including the cellular proteome, secretome, and glycoproteome using tandem mass spectrometry (MS/MS) of multiple fractions obtained from gel electrophoresis, multidimensional liquid chromatography, and solid phase extraction ...
Deniz, Baycin-Hizal, David L, Tabb, Raghothama, Chaerkady, Lily, Chen, Nathan E, Lewis, Harish, Nagarajan, Vishaldeep, Sarkaria, Amit, Kumar, Daniel, Wolozny, Joe, Colao, Elena, Jacobson, Yuan, Tian, Robert N, O'Meally, Sharon S, Krag, Robert N, Cole, Bernhard O, Palsson, Hui, Zhang, Michael, Betenbaugh   +17 more
openaire   +2 more sources