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Paraquat-resistant cell lines derived from chinese hamster ovary cells

Cell Biology International Reports, 1990
Two paraquat-resistant clones, PR-1 and PR-2, were selected from CHO K1 cells pretreated with ethyl methanesulfonate. PR-1 and PR-2, routinely cultured in a normal medium without paraquat, were six fold more resistant to paraquat than the parental CHO K1 cells.
T C, Lee   +6 more
openaire   +2 more sources

Regulation of hamster α1B-adrenoceptors expressed in Chinese hamster ovary cells

European Journal of Pharmacology, 1996
Chinese hamster ovary (CHO) cells were stably transfected to express the hamster alpha 1B-adrenoeceptor, and the function and agonist-induced regulation of the binding properties of these receptors were characterized. The cells expressed approximately 230,000 receptors per cell, with a KD for [3H]prazosin of 140 pM.
S J, Zhu   +3 more
openaire   +2 more sources

Purification of phosphatidylglycerophosphate synthase from Chinese hamster ovary cells

Biochemical Journal, 2001
Phosphatidylglycerophosphate (PGP) synthase catalyses the committed step in the biosynthesis of phosphatidylglycerol and cardiolipin in mammalian cells. Recently we isolated a Chinese hamster ovary (CHO) PGS1 cDNA encoding PGP synthase. In the present study we purified this PGP synthase to near-homogeneity from the mitochondrial fraction of CHO-K1 ...
K, Kawasaki   +3 more
openaire   +2 more sources

Ultrasound characterization of Chinese hamster ovary cells

The Journal of the Acoustical Society of America, 2012
Ultrasonic characteristics of various tissues are currently not known in enough detail to be used reliably for tissue identification or diagnosis. Analysis at a cellular level as opposed to a tissue level can examine these qualities specific to a cell type.
Aditya D. Mahajan   +3 more
openaire   +1 more source

Histones from Chinese hamster ovary cells

European Journal of Biochemistry, 1983
A quantitative analysis of histone variants and of their modified forms in exponentially growing Chinese hamster ovary cells is described. A two‐step analytical procedure has been developed. Total nuclear proteins were first fractionated on a sodium dodecyl sulfate/polyacrylamide slab gel electrophoresis.
G, Joseph   +2 more
openaire   +2 more sources

Chinese hamster ovary cells sensitivity to localized electrical stresses

Bioelectrochemistry and Bioenergetics, 1999
Application of an external electric field on a cell suspension induces an alteration in the membrane structure giving free access to the cell cytoplasm. Under mild pulsation conditions, permeabilization is a reversible process which weakly affects cell viability while drastic electrical conditions lead to cell death.
M C, Vernhes, P A, Cabanes, J, Teissie
openaire   +2 more sources

Electrofusion of Chinese hamster ovary cells after ethanol incubation

Biochimica et Biophysica Acta (BBA) - Biomembranes, 1985
Electrofusion of Chinese hamster ovary cells is obtained with cells growing in monolayers on a culture dish. The cells were incubated with increasing amounts of ethanol before the field pulsation. The electroperforation was apparently not affected by the incubation but the threshold field intensity required to induce the fusion was shifted to higher ...
G, Orgambide, C, Blangero, J, Teissié
openaire   +2 more sources

Proline biosynthesis: Multiple defects in Chinese hamster ovary cells

Biochemical and Biophysical Research Communications, 1973
Abstract The biochemical basis for the proline requirement of Chinese hamster ovary cells has been investigated. The results indicate an absence of conversion of glutamate to proline and a 90% reduction in ornithine transaminase, the first enzyme in the pathway converting ornithine to proline.
D, Valle   +3 more
openaire   +2 more sources

A Chinese hamster ovary cell mutant resistant to aphidicolin

Cell Biology International Reports, 1985
A triple (aphr ara-Ar and araCr) mutant (AP7) of Chinese hamster ovary cells resistant to DNA polymerase inhibitors is described. The aphidicolin-resistance of the mutant was stable and inherited as a dominant genetic trait. The DNA polymerase alpha from the wild type (aphs) and the mutant (aphr) cells differed in their elution profiles on DEAE ...
D B, Smith, N C, Mishra
openaire   +2 more sources

Genomic Exploration on Chinese Hamster Ovary Cells

2004
Industrially important recombinant CHO cell clones carry many desirable phenotypic traits that differentiate them from the parental clone. The molecular basis for these traits at the genomic level is not well understood. A better understanding of key genetic alterations related to these traits will greatly facilitate the development of new cell lines ...
M. De Leon Gatti   +13 more
openaire   +1 more source

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