Results 21 to 30 of about 128,450 (211)

Isolation and characterization of Chinese hamster ovary cell variants deficient in the expression of fibronectin receptor

Journal of Cell Biology, 1989
Chinese hamster ovary cell populations were enriched for cells displaying low surface expression of the 140-kD integrin fibronectin receptor (FnR) by means of fluorescence-activated cell sorting using monoclonal anti-FnR antibodies.
C. Schreiner, J. Bauer, Y. Danilov, S. Hussein, M. Sczekan, R. Juliano   +5 more
semanticscholar   +1 more source

A single mutation affects both N-acetylglucosaminyltransferase and glucuronosyltransferase activities in a Chinese hamster ovary cell mutant defective in heparan sulfate biosynthesis.

Proceedings of the National Academy of Sciences of the United States of America, 1992
Mutants of Chinese hamster ovary cells have been found that no longer produce heparan sulfate. Characterization of one of the mutants, pgsD-677, showed that it lacks both N-acetylglucosaminyl- and glucuronosyltransferase, enzymes required for the ...
K. Lidholt, J. L. Weinke, C. Kiser, F. N. Lugemwa, K. Bame, S. Cheifetz, J. Massagué, U. Lindahl, J. Esko   +8 more
semanticscholar   +1 more source

Amplified dihydrofolate reductase genes are localized to a homogeneously staining region of a single chromosome in a methotrexate-resistant Chinese hamster ovary cell line.

Proceedings of the National Academy of Sciences of the United States of America, 1978
Methotrexate-resistant Chinese hamster ovary cells selected for high resistance by progressive increments of methotrexate in the culture medium have levels of dihydrofolate reductase (tetrahydrofolate dehydrogenase, 7,8-dihydrofolate: NADP ...
J. Nunberg, R. Kaufman, R. Schimke, G. Urlaub, L. Chasin   +4 more
semanticscholar   +1 more source

Observation of Chinese Hamster Ovary Cells retained inside the non-woven fiber matrix of the CellTank bioreactor

Data in Brief, 2015
This data article shows how the recombinant Chinese Hamster Ovary (CHO) cells are located in the interstices of the matrix fibers of a CellTank bioreactor after completion of a perfusion culture, supporting the article entitled “Very high cell density ...
Ye Zhang, Véronique Chotteau
doaj   +1 more source

Assessment of the cytotoxicity of chlorhexidine by employing an in vitro mammalian test system

Journal of Dental Sciences, 2014
Background/purpose: Chlorhexidine (CHX), a chlorophenyl biguanide with broad antibacterial action, has been widely used in dentistry. The initial uses of CHX in dentistry were to wash operation site and to disinfect root canals. Recently, the addition of
Yi-Ching Li, Yu-Hsiang Kuan, Tzu-Hsin Lee, Fu-Mei Huang, Yu-Chao Chang   +4 more
doaj   +1 more source

Data-driven and Physics Informed Modelling of Chinese Hamster Ovary Cell Bioreactors [PDF]

arXiv, 2023
Fed-batch culture is an established operation mode for the production of biologics using mammalian cell cultures. Quantitative modeling integrates both kinetics for some key reaction steps and optimization-driven metabolic flux allocation, using flux balance analysis; this is known to lead to certain mathematical inconsistencies.
arxiv  

Glycomics Profiling of Chinese Hamster Ovary Cell Glycosylation Mutants Reveals N-Glycans of a Novel Size and Complexity*

Journal of Biological Chemistry, 2009
Identifying biological roles for mammalian glycans and the pathways by which they are synthesized has been greatly facilitated by investigations of glycosylation mutants of cultured cell lines and model organisms.
S. North, Hung‐Hsiang Huang, S. Sundaram, Jihye Jang-Lee, A. Etienne, Alana Trollope, Sara Chalabi, A. Dell, P. Stanley, S. Haslam   +9 more
semanticscholar   +1 more source

Osmotic Effects Induced by Pore-Forming Agent Nystatin: From Lipid Vesicles to the Cell. [PDF]

PLoS ONE, 2016
The responses of Chinese hamster ovary epithelial cells, caused by the pore-forming agent nystatin, were investigated using brightfield and fluorescence microscopy.
Špela Zemljič Jokhadar, Bojan Božič, Luka Kristanc, Gregor Gomišček   +3 more
doaj   +1 more source

Characterizing steady states of genome-scale metabolic networks in continuous cell cultures [PDF]

PLOS Computational Biology. 13 (11): e1005835 (2017-11-13), 2017
We present a model for continuous cell culture coupling intra-cellular metabolism to extracellular variables describing the state of the bioreactor, taking into account the growth capacity of the cell and the impact of toxic byproduct accumulation.
arxiv   +1 more source

BioPreDyn-bench: benchmark problems for kinetic modelling in systems biology [PDF]

BMC Systems Biology 2015 9:8, 2014
Dynamic modelling is one of the cornerstones of systems biology. Many research efforts are currently being invested in the development and exploitation of large-scale kinetic models. The associated problems of parameter estimation (model calibration) and optimal experimental design are particularly challenging.
arxiv   +1 more source