Results 51 to 60 of about 110,281 (284)

Lactylation‐Driven YTHDC1 Alleviates MASLD by Suppressing PTPN22‐Mediated Dephosphorylation of NLRP3

Advanced Science, EarlyView.
In MASLD, YTHDC1 undergoes increased lactylation and ubiquitination, reducing its expression. AARS1 mediates lactylation at lysine 565, while disrupted binding to LDHA further promotes lactylation, suppressing YTHDC1. This downregulation enhances PTPN22 mRNA stability, leading to NLRP3 dephosphorylation and activation, which exacerbates inflammation ...
Feng Zhang, Linghua Zeng, Kunkun Zou, Keying Lin, Fangting Yao, Jinglun Song, Shumeng Zhang, Hanshu Feng, Zhichao Yang, Chunlei Wang, Hongtao Diao, Xue Kong, Tengfei Pan, Joonki Kim, Tianqi Duo, Linqiang Li, Yu Bian   +16 more
wiley   +1 more source

Novel column generation-based optimization approach for poly-pathway kinetic model applied to CHO cell culture

Metabolic Engineering Communications, 2019
Mathematical modelling can provide precious tools for bioprocess simulation, prediction, control and optimization of mammalian cell-based cultures. In this paper we present a novel method to generate kinetic models of such cultures, rendering complex ...
Erika Hagrot, Hildur Æsa Oddsdóttir, Meeri Mäkinen, Anders Forsgren, Véronique Chotteau   +4 more
doaj   +1 more source

Isolation and characterization of Chinese hamster ovary cell mutants defective in intracellular low density lipoprotein-cholesterol trafficking

Journal of Cell Biology, 1990
This paper reports the isolation and characterization of Chinese hamster ovary cell mutants defective in low density lipoprotein (LDL)- cholesterol trafficking.
K. Cadigan, D. Spillane, T. Chang
semanticscholar   +1 more source

Remote Modulation of Single‐Atom Catalyst Boosts High‐Valent Cobalt–Oxo Species Generation for Water Purification and Detoxification

Advanced Science, EarlyView.
This study develops a phosphorus‐doped cobalt single‐atom catalyst that enhances high‐valent cobalt–oxo species generation for water purification. This atomic‐level tuning boosts the catalyst's activity by 3.5 times, enabling efficient pollutant degradation. A continuous‐flow reactor achieves high, long‐term removal efficiency, producing water with low
Wen‐Min Wang, Zheng‐Wei Yang, De‐Xiu Wu, Wen‐Long Wang, Qian‐Yuan Wu   +4 more
wiley   +1 more source

Application of a genome-scale model in tandem with enzyme assays for identification of metabolic signatures of high and low CHO cell producers

Metabolic Engineering Communications, 2019
Biopharmaceutical industrial processes are based on high yielding stable recombinant Chinese Hamster Ovary (CHO) cells that express monoclonal antibodies.
Cyrielle Calmels, Solène Arnoult, Bassem Ben Yahia, Laetitia Malphettes, Mikael Rørdam Andersen   +4 more
doaj   +1 more source

Versatile microscale screening platform for improving recombinant protein productivity in Chinese hamster ovary cells [PDF]

, 2015
Chinese hamster ovary (CHO) cells are widely used as cell factories for the production of biopharmaceuticals. In contrast to the highly optimized production processes for monoclonal antibody (mAb)-based biopharmaceuticals, improving productivity of non ...
Andersen, Mikael Rørdam, Grav, Lise Marie, Hansen, Henning Gram, Kildegaard, Helene Faustrup, Kol, Stefan, Lund, Anne Mathilde, Lundqvist, Magnus, Min Lee, Gyun, Nilsson, Claes Nymand, Rockberg, Johan   +9 more
core   +1 more source

Failure to release iron from transferrin in a Chinese hamster ovary cell mutant pleiotropically defective in endocytosis

Journal of Cell Biology, 1984
A Chinese hamster ovary cell mutant defective in the receptor-mediated endocytosis of several unrelated ligands (Robbins, A. R., S. S. Peng, and J. L. Marshall, 1983, J. Cell Biol., 96:1064-1071) failed to accumulate iron provided in the form of diferric
R. Klausner, J. V. Renswoude, C. Kempf, K. Rao, J. Bateman, April R. Robbins   +5 more
semanticscholar   +1 more source

Quantitative Proteomics and CRISPR/Cas9 Editing Reveal UPR‐Mediated Control of Immunoglobulin Homeostasis in Hybridomas

Advanced Science, EarlyView.
BCR sequencing and subclone analysis correlated immunoglobulin (Ig) chain loss in dysfunctional hybridomas with disrupted monoclonal antibody homeostasis. Proteomics‐guided CRISPR/Cas9 editing revealed that the unfolded protein response (UPR) regulates aberrant Ig synthesis.
Rubing Zou, Xinying Lu, Ying Liu, Peiyu Yang, Shuo Sun, Yihua Liu, Yinyuan Mo, Guonian Zhu, Jae Seong Lee, Yirong Guo   +9 more
wiley   +1 more source

Isolation and characterization of Chinese hamster ovary cell mutants defective in assembly of peroxisomes

Journal of Cell Biology, 1990
We made use of autoradiographic screening to isolate two Chinese hamster ovary (CHO) cell mutants deficient in peroxisomal dihydroxyacetonephosphate acyltransferase, a key enzyme for the biosynthesis of ether glycerolipids such as plasmalogens ...
T. Tsukamoto, S. Yokota, Y. Fujiki
semanticscholar   +1 more source

Autosomal Dominant Erythrocytosis Caused by Non‐Renal Erythropoietin (EPO) Due to EPO c.‐136 G>A Germline Mutation

American Journal of Hematology, EarlyView.
A novel erythropoietin (EPO) promoter mutation (c.‐136 G>A) causes autosomal dominant erythrocytosis via non‐renal expression of EPO. ABSTRACT We previously reported a five‐generation kindred with autosomal dominant erythrocytosis associated with a novel germline promoter variant in the erythropoietin (EPO) gene (EPO c.‐136 G>A).
Lucie Lanikova, Dusan Hrckulak, Veronika Zimolova, Felipe R. Lorenzo, Jihyun Song, Katerina Vecerkova, Olga Babosova, Linda Berkova, Vladimir Korinek, Steve Elliott, Josef T. Prchal   +10 more
wiley   +1 more source