Results 51 to 60 of about 43,674 (210)

Performance Comparison between Semi‐Automated and Manual Cell Counting for Animal Cell Culture

Chemie Ingenieur Technik, EarlyView.
Following ISO 20391‐2:2019 guidelines, manual and semi‐automated cell counting performances were compared using validation parameters from ICH Q2(R1). Specificity, linearity, and repeatability served as key validation parameters in the evaluation of total cell density and cell viability of CHO‐K1 cells using trypan blue exclusion staining.
Selina Uhlendorff, Ulrich Odefey, Björn Frahm, Miriam Pein‐Hackelbusch   +3 more
wiley   +1 more source

Thrombomodulin is essential for recombinant Fc-fusion protein production in Chinese hamster ovary cells via multiple signaling pathways

Journal of Biological Engineering
Background Chinese hamster ovary (CHO) cells are the predominant cell line used for biotherapeutic production. To reduce the cost of therapeutic recombinant proteins produced in CHO cells, efforts have been made over decades to improve overall yield ...
Xiaonan Ma, Nawei Wang, Qihang Yan, Shuang Li, Guorong Hu, Lunxiao Zhao, Ruoyin Xiao, Mingqi Wang, Ningning Ma   +8 more
doaj   +1 more source

Protocol for microfluidic single-cell cultivation and live-cell imaging of Chinese hamster ovary suspension cell lines

STAR Protocols
Summary: Microfluidic single-cell cultivation (MSCC) is a powerful tool for investigating the cellular behavior of various cell types at the single-cell level.
Julian Schmitz, Boris Yermakov, Alexander Grünberger   +2 more
doaj   +1 more source

Chemical engineering as an essential element of industrial biotechnology in Mexico: New aims in research and university education

The Canadian Journal of Chemical Engineering, EarlyView.
Abstract On the centennial of higher education in Chemical Engineering in Mexico, it is pertinent to revisit the key stages that have contributed to its consolidation as a vital discipline for the nation's scientific and technological advancement. Although the initial mission of chemical engineering education was primarily oriented toward the training ...
Agustín López Munguía, Octavio T. Ramírez, Rafael Vazquez‐Duhalt, Eduardo Bárzana   +3 more
wiley   +1 more source

The In Vitro Transgenic Rodent Assay in Primary MutaMouse Hepatocytes Compared to the Mammalian Cell Gene Mutation Assay Using the HPRT Gene

Environmental and Molecular Mutagenesis, EarlyView.
ABSTRACT Gene mutations can be detected in mammalian cells in vitro using indicator genes such as the hypoxanthine‐guanine‐phosphoribosyltransferase (HPRT) gene. These assays have been adopted as OECD test guidelines (TG, e.g., OECD TG no. 476) and are used for regulatory purposes.
Alina Göpfert, Kylee Kendra Marie Ronnenberg, Claudia Ruelker, Silke Spang, Naveed Honarvar, Robert Landsiedel   +5 more
wiley   +1 more source

26-hydroxycholesterol: regulation of hydroxymethylglutaryl-CoA reductase activity in Chinese hamster ovary cell culture.

Journal of Lipid Research, 1983
The effect of 26-hydroxycholesterol and other intermediates in bile acid synthesis on HMG-CoA reductase activity was studied in Chinese hamster ovary (CHO) cell culture.
A L Esterman, H Baum, N B Javitt, G J Darlington   +3 more
doaj   +1 more source

Neurodevelopmental features in KCNQ2 developmental and epileptic encephalopathy may have limited associations with KV7.2 dysfunction

Epilepsia Open, EarlyView.
Abstract Objective Variants in KCNQ2 encoding the voltage‐gated potassium channel KV7.2 are associated with developmental and epileptic encephalopathy (DEE) of varying severity. This study examined the relationship of KCNQ2 variant dysfunction with the neurodevelopmental phenotype of individuals with KCNQ2‐DEE. Methods A parent‐reported survey gathered
Jessa S. Bidwell, Carlos G. Vanoye, Reshma R. Desai, Anne T. Berg, Alfred L. George Jr   +4 more
wiley   +1 more source

Comparison of the Production of Recombinant Protein in Suspension Culture of CHO Cells in Spinner Flask and Shake Flask System

International Islamic University Malaysia Engineering Journal, 2011
Chinese hamster ovary (CHO) cells have been most widely used as the production host for the commercial production of biopharmaceuticals product.
S.N.Z Zainul Abidin, and N. Anuar
doaj   +1 more source

Using generalized quantitative structure–property relationship (QSPR) models to predict host cell protein retention in ion‐exchange chromatography

Journal of Chemical Technology &Biotechnology, EarlyView.
Abstract BACKGROUND Selecting an optimal chromatography resin during biopharmaceutical downstream process development is a great challenge. This is especially the case for recombinant subunit vaccines, where product properties vary greatly and recovery often involves cell lysis, which yields a complex mixture of different host cell materials. Host cell
Tim Neijenhuis, Olivier Le Bussy, Geoffroy Geldhof, Marieke E Klijn, Marcel Ottens   +4 more
wiley   +1 more source

Rapid Determination of Membrane Transport Parameters in Adherent Cells

BioTechniques, 2000
Reported here is a new method that permits rapid (approximately 5 s) determinations of membrane transport phenomena in cells grown in monolayers at the base of 17-mm glass scintillation vials.
Karim A. Sharif, I. David Goldman
doaj   +1 more source