Results 61 to 70 of about 110,281 (284)

In vitro approach to the study of chronic exposure to low doses of x-rays [PDF]

, 2011
El presente trabajo fue realizado con el fin de estudiar el efecto de dosis bajas y repetidas de radiación sobre dos líneas celulares de la misma especie. Se desarrolló un modelo in vitro para evitar la influencia de los factores de confusión que afectan
Peral Garcia, Pilar, Ponzinibbio, Maria Virginia, Seoane, Analia Isabel   +2 more
core   +1 more source

Chinese Hamster Ovary Cell Mutants Defective in Glycosaminoglycan Assembly and Glucuronosyltransferase I*

Journal of Biological Chemistry, 1999
The proteoglycans of animal cells typically contain one or more heparan sulfate or chondroitin sulfate chains. These glycosaminoglycans assemble on a tetrasaccharide primer, -GlcAβ1,3Galβ1,3Galβ1,4Xylβ-O-, attached to specific serine residues in the core
X. Bai, G. Wei, A. Sinha, J. Esko
semanticscholar   +1 more source

Dynamic Binder Exchange Improves Protein Labeling Efficiency in DNA‐PAINT up to 15‐Fold

Angewandte Chemie International Edition, EarlyView.
Dynamic Binder Exchange (DyBE) leverages transient binder–target interactions to enhance labeling efficiency by up to 15‐fold in super‐resolution microscopy. DyBE maps nanoscale receptor tyrosine kinase (RTK) assemblies, including HER2 homodimers and EGFR–HER2 heterodimers, advancing single‐protein spatial proteomics.
Clemens Steinek, Isabelle Pachmayr, Sebastian Strauss, Monique Honsa, Jisoo Kwon, Ralf Jungmann   +5 more
wiley   +1 more source

Caspase-7 deficiency in Chinese hamster ovary cells reduces cell proliferation and viability

Biological Research, 2020
Background Chinese hamster ovary (CHO) cells are the most commonly used mammalian host cell in the commercial-scale production of biopharmaceutical proteins. Modification of genes involved in apoptosis may improve the productivity of CHO cells. Executive
Fatemeh Safari, Safar Farajnia, Abbas Behzad Behbahani, Habib Zarredar, Mazyar Barekati-Mowahed, Hesam Dehghani   +5 more
doaj   +1 more source

Transient Transfection of Rolling‐Circle Amplified DNA in Biomanufacturing‐Relevant Mammalian Cell Lines: A Comparison of Transfection Conditions for Optimal Protein Expression

Biotechnology and Applied Biochemistry, EarlyView.
ABSTRACT Rolling‐circle amplification (RCA) is a completely synthetic and rapid mechanism for scaling‐up bulk DNA that eliminates requirements for large‐scale bacterial fermentation. Several reports have described the surprising ability of large unprocessed (hyperbranched) rolling circle‐amplified DNA (RCA DNA) to effectively transfect cultured cells ...
Evelina Loghin, Erik Kvam, Lisa Lowery, Nathan Hudson, Jenny Keen, Brian Davis, Roger B. Dodd, Christine Surrette, John Nelson   +8 more
wiley   +1 more source

Model-based analysis of N-glycosylation in Chinese hamster ovary cells. [PDF]

PLoS ONE, 2017
The Chinese hamster ovary (CHO) cell is the gold standard for manufacturing of glycosylated recombinant proteins for production of biotherapeutics. The similarity of its glycosylation patterns to the human versions enable the products of this cell line ...
Frederick J Krambeck, Sandra V Bennun, Mikael R Andersen, Michael J Betenbaugh   +3 more
doaj   +1 more source

A natural killer cell receptor specific for a major histocompatibility complex class I molecule. [PDF]

, 1994
Target cell expression of major histocompatibility complex (MHC) class I molecules correlates with resistance to lysis by natural killer (NK) cells.
Daniels, BF, Karlhofer, FM, Seaman, WE, Yokoyama, WM   +3 more
core  

Expression of Kv1.1 Delayed Rectifier Potassium Channels in Lec Mutant Chinese Hamster Ovary Cell Lines Reveals a Role for Sialidation in Channel Function*

Journal of Biological Chemistry, 1996
Kv1.1 potassium (K+) channels contain significant amounts of negatively charged sialic acids. To examine the role of sialidation in K+ channel function, Chinese hamster ovary cell lines deficient in glycosylation (Lec mutants) were transfected with rat ...
W. Thornhill, Michael B. Wu, Xiaoqiao Jiang, Xiaying Wu, Peter T. Morgan, J. Margiotta   +5 more
semanticscholar   +1 more source

Lab‐Scale Continuous Biomanufacturing: A Tool for Process Development With Adaptive Strategies for Capture and Virus Inactivation

Biotechnology and Bioengineering, EarlyView.
Schematic representation of the continuous lab‐scale process. ABSTRACT Monoclonal antibodies (mAbs) are nowadays fundamental in treating a wide range of severe diseases, including cancer, infections, or autoimmune disorders. Due to their high specificity, potent activity, and fewer side effects compared to small molecular drugs, the market for mAbs is ...
Thomas Kruse, Fabian Schmitz, Janina Kilian, Jonas Austerjost, Lucas‐Nik Reger, Markus Kampmann   +5 more
wiley   +1 more source

Sequencing, annotation and analysis of the Syrian hamster (Mesocricetus auratus) transcriptome. [PDF]

PLoS ONE, 2014
BACKGROUND:The Syrian hamster (golden hamster, Mesocricetus auratus) is gaining importance as a new experimental animal model for multiple pathogens, including emerging zoonotic diseases such as Ebola.
Nicolas Tchitchek, David Safronetz, Angela L Rasmussen, Craig Martens, Kimmo Virtaneva, Stephen F Porcella, Heinz Feldmann, Hideki Ebihara, Michael G Katze   +8 more
doaj   +1 more source