Results 71 to 80 of about 110,281 (284)
Agrin isoforms and their role in synaptogenesis [PDF]
, 1992 Agrin is thought to mediate the motor neuron-induced aggregation of synaptic proteins on the surface of muscle fibers at neuromuscular junctions. Recent experiments provide direct evidence in support of this hypothesis, reveal the nature of agrin ...
Escher, Gérard +6 more
core +1 more source
Biotechnology and Bioengineering, EarlyView.ABSTRACT Polysorbate‐degrading host cell proteins (HCPs) represent a critical challenge in the manufacturing of monoclonal antibody therapeutics due to their potential to persist during downstream processing. While their enzymatic activity has been characterized, the role of direct HCP‐mAb interactions, particularly those involving polysorbate ...
Melanie Maier +3 more
wiley +1 more source
مجله دانشکده پزشکی اصفهان, 2012 Background: Improvement of mRNA stability and therefore, production of interferon beta (IFNb) in Chinese hamster ovary (CHO) cell, as a recombinant protein expression system, is very important.
Zahra Bezi +5 more
doaj
Radiology and OncologyAdministering gadolinium-based contrast agent before electroporation allows the contrast agent to enter the cells and enables MRI assessment of reversibly electroporated regions.
Strucic Marko +5 more
doaj +1 more source
Investigating Virus Clearance via pH Inactivation During Biomanufacturing [PDF]
, 2019 In the processing of biopharmaceuticals, viral clearance and viral safety are important for the development of monoclonal antibodies. Murine xenotropic leukemia virus (XMuLV) is one of the retroviruses, recommended by Food and Drug Administration (FDA ...
Xu, Wenbo
core +2 more sources
Fluorescent turn-on probes for wash-free mRNA imaging via covalent site-specific enzymatic labeling. [PDF]
, 2017 Investigating the many roles RNA plays in cellular regulation and function has increased demand for tools to explore RNA tracking and localization within cells.
Alexander, Seth C +2 more
core +2 more sources
Biotechnology Progress, EarlyView.Abstract Key hydrodynamic‐related parameters such as volumetric power input (P/V), impeller configuration, aeration strategy, and maximum gas sparge rate, as well as an appropriate feeding strategy, must be carefully selected to improve production yields in bioreactor.
Lucas Lemire +5 more
wiley +1 more source
BioTechniques, 2021 Cellular redox changes are common in apoptosis, immune function, signaling pathways and cancer. The authors aimed to develop a single-wavelength method using the superior fluorescence sensitivity of a flow cytometer for measuring redox-sensitive green ...
Elizabeth R Denn, Joseph M Schober
doaj +1 more source
Dynamic pH profiles drive higher cell‐specific and volumetric productivity
Biotechnology Progress, EarlyView.Implementation of dyamic pH profiles for increased CHO cell culture productivity. Abstract Mammalian cell cultures in bioreactors rely heavily on critical process parameter control to ensure optimal growth, productivity, and reproducibility to produce recombinant therapeutic proteins. Culture pH has been shown to be a critical parameter that influences
Stephanie R. Klaubert +6 more
wiley +1 more source
What can mathematical modelling say about CHO metabolism and protein glycosylation?
Computational and Structural Biotechnology Journal, 2017 Chinese hamster ovary cells have been in the spotlight for process optimization in recent years, due to being the major, long established cell factory for the production of recombinant proteins.
Sarah N. Galleguillos +5 more
doaj +1 more source