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Studies on Hexosaminidase Forms in Chinese Hamster Ovary Cells
Enzyme, 2017The hexosaminidase forms present in Chinese hamster ovary cells grown in culture were partially purified and their kinetic and physical properties were characterized. The cells were found to contain three hexosaminidase forms, which could be resolved by ion-exchange chromatography.
O W, Blaschuk +2 more
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Conserved MicroRNAs in Chinese hamster ovary cell lines
Biotechnology and Bioengineering, 2010AbstractMicroRNAs (miRNAs), a class of short (20–24 nt) non‐coding RNAs that direct post‐transcriptional repression of messenger RNAs, increasingly have been shown to play a key role in regulating cellular physiology. We investigated the prevalence of miRNAs in Chinese hamster ovary (CHO) cells by high‐throughput sequencing. Six cDNA libraries of small
Kathryn C, Johnson +6 more
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Metabolism and action of neplanocin A in Chinese hamster ovary cells
Biochemical Pharmacology, 1985Neplanocin A is a naturally occurring carbocyclic analog of adenosine which contains a cyclopentene moiety in place of ribose and has demonstrated antitumor and antimicrobial activity. This compound was highly toxic to Chinese hamster ovary (CHO) cells; the approximate minimum inhibitory concentration of neplanocin A for inhibition of clone formation ...
P P, Saunders, M T, Tan, R K, Robins
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A Chinese hamster ovary cell mutant resistant to aphidicolin
Cell Biology International Reports, 1985A triple (aphr ara-Ar and araCr) mutant (AP7) of Chinese hamster ovary cells resistant to DNA polymerase inhibitors is described. The aphidicolin-resistance of the mutant was stable and inherited as a dominant genetic trait. The DNA polymerase alpha from the wild type (aphs) and the mutant (aphr) cells differed in their elution profiles on DEAE ...
D B, Smith, N C, Mishra
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Cytotoxicity and mutagenicity of dinitrotoluenes in Chinese hamster ovary cells
Mutation Research Letters, 1982Technical grade dinitrotoluene (DNT), a mixture composed predominantly of 2,4- and 2,6-DNT but containing lesser amounts of 2,3-, 2,5-, 3,4- and 3,5-DNT, has been shown to be a hepatocarcinogen in rats, The mutagenicity of these compounds has been evaluated using the CHO/HGPRT system, a quantitative mammalian somatic cell mutational assay ...
D J, Abernethy, D B, Couch
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Metabolomics of Chinese Hamster Ovary Cells
Increasing demand of protein biotherapeutics produced using Chinese hamster ovary (CHO) cell lines necessitates improvement in the production yield of the bioprocess. Various cell engineering, improved media formulation and process-design based approaches utilizing the power of OMICS technologies, specifically, genomics and proteomics, have been ...Rita, Singh +5 more
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The selection of virus-resistant Chinese hamster ovary cells
Cell, 1976We have selected Chinese hamster ovary (CHO) cells resistant to infection by encephalomycarditis (EMC) virus. Thus far, we have obtained five lines resistant to EMC, all of which manifest different phenotypes. Three of the five are not persistently infected with virus, while two lines produce infectious virus and grow in its presence.
R, Taber, V, Alexander, N, Wald
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Hyperthermic Radiosensitization of Thermotolerant Chinese Hamster Ovary Cells
Radiation Research, 1986Synchronous G1 cells were given a priming dose of heat (45.5 degrees C for 15 min) and then heated and irradiated 6-120 h later. Compared to heat radiosensitization for cells irradiated 10 min after the priming heat dose (thermal enhancement ratio, TER of 2.6 for a 10-fold reduction in survival), heat radiosensitization 18-24 h after the priming heat ...
P K, Holahan +3 more
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Biallelic Gene Knockouts in Chinese Hamster Ovary Cells
2008Chinese hamster ovary (CHO) cells are the most common host cells and are widely used in the manufacture of approved recombinant therapeutics. They represent a major new class of universal hosts in biopharmaceutical production. However, there remains room for improvement to create more ideal host cells that can add greater value to therapeutic ...
Naoko, Yamane-Ohnuki +2 more
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Nucleoside kinase activities of Chinese hamster ovary cells
Biochimica et Biophysica Acta (BBA) - General Subjects, 1983Chinese hamster ovary (CHO) cells and appropriate drug-resistant mutants derived from them have been analyzed for nucleoside kinase activities relevant to the phosphorylation of adenosine, deoxyadenosine, deoxyguanosine and deoxycytidine and for resistance to a variety of nucleoside analogs.
P P, Saunders, M M, Lai
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