Results 211 to 220 of about 5,769,168 (249)
Some of the next articles are maybe not open access.
Developing genomic platforms for Chinese hamster ovary cells
Biotechnology Advances, 2009Chinese hamster ovary (CHO) cells are widely used in recombinant protein production, yet despite their importance in bioprocessing, few genomic resources have been developed for this cell line. Over the past several years, we have made considerable progress in the development of genomic tools for CHO.
Anne, Kantardjieff +7 more
openaire +2 more sources
Regulation of polyamine transport in Chinese hamster ovary cells
Journal of Cellular Physiology, 1990AbstractControl Chinese hamster ovary (CHO) cells and mutant CHO cells lacking ornithine decarboxylase activity (CHODC−) were used to study the regulation of polyamine uptake. It was found that the transport system responsible for this uptake was regulated by intracellular polyamine levels and that this regulation was responsible for the maintenance of
T L, Byers, A E, Pegg
openaire +2 more sources
[Modification of Chinese hamster ovary cells].
Sheng wu gong cheng xue bao = Chinese journal of biotechnology, 2002Chinese hamster ovary cells (CHO) are preferable to prokaryotic, yeast or insect cells as hosts for biopharmaceutical production due to the products are more similar to their natural conformation. However, CHO cells confront tremendous difficulties when cultured in large scale such as mal-adaptation to serum-free medium, apoptosis and over-growth ...
Da-Zhi, Lai +4 more
openaire +1 more source
Phagocyte-induced mutation in Chinese hamster ovary cells
Cancer Letters, 1984Human phagocytic cells elaborate toxic oxygen metabolites which can cause mutations in bacteria and sister chromatid exchanges in cultured mammalian cells. In the present study we demonstrate that human phagocytes can induce mutations in Chinese hamster ovary cells.
S A, Weitzman, T P, Stossel
openaire +2 more sources
[1] Chinese hamster ovary cells
1987Publisher Summary This chapter describes Chinese hamster ovary (CHO) cells that use for genetic analysis in tissue culture. These cells have been used for the isolation of mutants affecting intermediary metabolism; DNA, RNA, and protein synthesis; membrane functions; and several more complex forms of cell behavior such as cell growth and endocytosis.
openaire +1 more source
Reaching the depth of the Chinese hamster ovary cell transcriptome
Biotechnology and Bioengineering, 2010AbstractThe high‐throughput DNA sequencing Illumina Solexa GAII platform was employed to characterize the transcriptome of an antibody‐producing Chinese hamster ovary (CHO) cell line. More than 55 million sequencing reads were generated and mapped to an existing set of CHO unigenes derived from expressed sequence tags (ESTs), as well as several public ...
Nitya M, Jacob +7 more
openaire +2 more sources
Regulation of transglutaminase activity in Chinese hamster ovary cells
Biochimica et Biophysica Acta (BBA) - General Subjects, 1980We have investigated the regulation of transglutaminase activity (epsilon-(gamma-glutamyl)lysine crosslinking enzyme) in Chinese hamster ovary cells in culture. We report that transglutaminase activity increases several-fold in CHO cells at maximum density in suspension culture. This increase cannot be explained by the presence of soluble regulators of
P G, Milhaud +3 more
openaire +2 more sources
Characterization of peroxisomes in Chinese hamster ovary cells in culture
Experimental Cell Research, 1985In order to explore the potential value of Chinese hamster ovary (CHO) cells for the isolation of peroxisomal mutants defective in the peroxisomal fatty acid oxidation system, some characteristics of their peroxisomes were studied. Catalase was detected biochemically and histochemically in peroxisome-like particles in cells or in subcellular fractions ...
SANTOS, MJ +4 more
openaire +2 more sources
Surface polypeptides of the cultured Chinese hamster ovary cell
Biochemistry, 1975The organization of the plasma membrane of logarithmically growing Chinese hamster ovary (CHO) suspension cells has been probed using surface label techniques in conjunction with subcellular fractionation and sodium dodecyl sulfate gel electrophoresis. Five components of apparent molecular weights 137,000, 121,000, 97,000, 67,000, and 57,000 have been ...
R L, Juliano, M, Behar-Bannelier
openaire +2 more sources
The transport of L-arginine in Chinese hamster ovary cells
Biochemical and Biophysical Research Communications, 1989The transport of L-arginine has been characterized in Chinese hamster ovary cells (CHO). In the absence of Na+ the influx of the amino acid decreased. Both in the presence and in the absence of Na+ L-arginine influx was trans-stimulated and cis-inhibited by cationic amino acids.
ROTOLI, Bianca Maria +3 more
openaire +3 more sources