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Cell Engineering and Cultivation of Chinese Hamster Ovary (CHO) Cells
Current Pharmaceutical Biotechnology, 2010Mammalian cell lines are important host cells for the industrial production of pharmaceutical proteins owing to their capacity for correct folding, assembly and post-translational modification. In particular, Chinese hamster ovary (CHO) cells are the most dependable host cells for the industrial production of therapeutic proteins.
Takeshi, Omasa +2 more
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Regulation of hamster α1B-adrenoceptors expressed in Chinese hamster ovary cells
European Journal of Pharmacology, 1996Chinese hamster ovary (CHO) cells were stably transfected to express the hamster alpha 1B-adrenoeceptor, and the function and agonist-induced regulation of the binding properties of these receptors were characterized. The cells expressed approximately 230,000 receptors per cell, with a KD for [3H]prazosin of 140 pM.
S J, Zhu +3 more
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Glycerolization of Chinese hamster ovary cells in monolayer culture
Cryobiology, 1980Abstract CHO monolayer cultures were used as a model system to examine the kinetics of cell glycerolization and deglycerolization. Both influx and efflux of [3H]glycerol appear to be first-order processes, the rate of flux being proportional to glycerol concentration.
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Perfusion culture of Chinese Hamster Ovary cells for bioprocessing applications
Critical Reviews in Biotechnology, 2022Dinora Roche Recinos +2 more
exaly
Electrotransformation of Chinese Hamster Ovary Cells
2003D G, Taghian, J A, Nickoloff
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Production and Purification of Antibodies in Chinese Hamster Ovary Cells
Antibodies are versatile biological molecules with widespread applications in research and medicine. This protocol outlines the generation of monoclonal IgG antibodies from Chinese hamster ovary cells. It includes steps for cell maintenance, transient transfection, and antibody purification via protein A affinity chromatography.openaire +2 more sources
HPRT Mutation Assay for Chinese Hamster Ovary Cells
Gene mutations are a hallmark of cancer. Although mutations can occur due to various endogenous and extracellular stress factors daily, detecting them is not straightforward. The HPRT gene is one of the housekeeping genes and is essential for the nucleotide salvage pathway.openaire +2 more sources
REFERENCE RECOMBINANT CHINESE HAMSTER OVARY CELL LINES
In Vitro Cellular & Developmental Biology - Animal, 2001C H, Liu, I M, Chu, S M, Hwang
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