Results 71 to 80 of about 83,358 (225)

Dynamic pH profiles drive higher cell‐specific and volumetric productivity

Biotechnology Progress, EarlyView.
Implementation of dyamic pH profiles for increased CHO cell culture productivity. Abstract Mammalian cell cultures in bioreactors rely heavily on critical process parameter control to ensure optimal growth, productivity, and reproducibility to produce recombinant therapeutic proteins. Culture pH has been shown to be a critical parameter that influences
Stephanie R. Klaubert, Dylan G. Chitwood, Danqia Peng, Erin Redman, Ji Young L. Anderson, Nicholas R. Sandoval, Sarah W. Harcum   +6 more
wiley   +1 more source

Enhancing CHO cell recombinant protein production using a perfusion‐directed host evolution approach

Biotechnology Progress, EarlyView.
Abstract Clonally derived cell lines generated from Chinese hamster ovary (CHO) cells encounter numerous stressors when cultured in high‐intensity perfusion bioreactors leading to poor process performance. To circumvent this, the ability of CHO cells to adapt to different culture environments was exploited. Here host cells were selected in the presence
Peter Amaya, Rajesh K. Mistry, Susie Sou, Sonja Hess, Bijay Khanal, Zayla Schaeffer, Jie Zhu, Lina Chakrabarti   +7 more
wiley   +1 more source

Analysis of magnetic resonance contrast agent entrapment following reversible electroporation in vitro

Radiology and Oncology
Administering gadolinium-based contrast agent before electroporation allows the contrast agent to enter the cells and enables MRI assessment of reversibly electroporated regions.
Strucic Marko, Miklavcic Damijan, Vidic Zala, Scuderi Maria, Sersa Igor, Kranjc Matej   +5 more
doaj   +1 more source

Rapid Determination of Membrane Transport Parameters in Adherent Cells

BioTechniques, 2000
Reported here is a new method that permits rapid (approximately 5 s) determinations of membrane transport phenomena in cells grown in monolayers at the base of 17-mm glass scintillation vials.
Karim A. Sharif, I. David Goldman
doaj   +1 more source

Agrin isoforms and their role in synaptogenesis [PDF]

, 1992
Agrin is thought to mediate the motor neuron-induced aggregation of synaptic proteins on the surface of muscle fibers at neuromuscular junctions. Recent experiments provide direct evidence in support of this hypothesis, reveal the nature of agrin ...
Escher, Gérard, Honig, Lawrence S., Horton, Sharon E., Kröger, Stephan, McMahan, U. J., Ruegg, Markus A., Werle, Michael J.   +6 more
core   +1 more source

A case study showing the role of hydrophobicity variants and other enriched mAb proteoforms on filterability through a virus filter with productivity improvement measures

Biotechnology Progress, EarlyView.
Abstract A rapid assessment of manufacturability for drug candidates is crucial for advancing a prospective biotherapeutic from a candidate to a bulk drug substance. A lot‐to‐lot approach to manufacturability is adopted where each biologic batch is assessed for manufacturability as a bulk, unfractionated pool.
Solomon Isu, Derek Silva, Melissa Holstein, Angela Lewandowski, Kristina Cunningham, Adam Sokolnicki, Bala Raghunath   +6 more
wiley   +1 more source

High throughput, efficacious gene editing & genome surveillance in Chinese hamster ovary cells.

PLoS ONE, 2019
Chinese hamster ovary (CHO) cells are a common tool utilized in bioproduction and directed genome engineering of CHO cells is of great interest to enhance recombinant cell lines.
S C Huhn, Y Ou, A Kumar, R Liu, Z Du
doaj   +1 more source

The GalNAc-type O-Glycoproteome of CHO Cells Characterized by the SimpleCell Strategy [PDF]

, 2014
The Chinese hamster ovary cell (CHO) is the major host cell factory for recombinant production of biological therapeutics primarily because of its “human-like” glycosylation features.
Alder Schulz, Morten, Clausen, Henrik, Gram Schjoldager, Katrine Ter-Borch, Halim, Adnan, Jitendra Joshi, Hiren, Kayser, Kevin J., Levery, Steven B., Narimatsu, Yoshiki, Paul Bennett, Eric, Sealover, Natalie R., Steentoft, Catharina, Vakhrushev, Sergey Y., Zhang, Yang   +12 more
core   +1 more source

Evaluation of gene editing in CHO cells using the Cas‐CLOVER system

Biotechnology Progress, EarlyView.
Abstract Recent advances in gene editing technologies have transformed the genetic engineering of Chinese hamster ovary (CHO) hosts, enabling the development of cell lines with improved stability and productivity. In this study, we employed the programmable nuclease (PN) Cas‐CLOVER to precisely target the Glutamine synthetase (GS) locus in CHO cells. A
Tiffany McLamarrah, Efecan Aral, Michael Hoffman, Jennifer Tedstone, Thomas King, Jason Vitko, Maria João Sebastião, Jose M. Escandell, Mafalda M. Dias, Iona McCall, Daniel Machado, Victor Cairns, Christine DeMaria, John J. Scarcelli   +13 more
wiley   +1 more source

Methyl-α-D-glucopyranoside from Tulbaghia violacea extract induces apoptosis in vitro in cancer cells

Bangladesh Journal of Pharmacology, 2013
Pro-apoptotic activity of Tulbghia violacea extracts was evaluated in order to understand the mechanisms of action that might be related to its traditional use as anticancer medicine.
Sylvester Leonard Lyantagaye
doaj   +1 more source