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Chitin deacetylases: new, versatile tools in biotechnology
Trends in Biotechnology, 2000Chitin deacetylases have been identified in several fungi and insects. They catalyse the hydrolysis of N-acetamido bonds of chitin, converting it to chitosan. Chitosans, which are produced by a harsh thermochemical procedure, have several applications in areas such as biomedicine, food ingredients, cosmetics and pharmaceuticals.
I, Tsigos +3 more
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Chitin deacetylase product inhibition
Biotechnology Journal, 2010AbstractChitin deacetylase is the only known enzyme catalyzing the hydrolysis of the acetamino linkage in the N‐acetylglucosamine units of chitin and chitosan. This reaction can play an important role in enzymatic production of chitosan from chitin, or in enzymatic modification of chitosan, which has applications in medicine, pharmacy or plant ...
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Chitin Deacetylases in Invertebrates
1986Chit in deacetylase activities have been characterised from fungi in those cases where the resulting chitosan forms a structural component of the wall, and from chitinoclastic bacteria. Here are two accounts of their activity in invertebrates, being apparently associated here in softening of chitinous tissues to allow their expansion.
G. W. Gooday +3 more
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Purification and Characterization of Chitin Deacetylase from Absidia coerulea
Journal of Biochemistry, 1995Chitin deacetylase, which releases the acetyl groups of glycol chitin was purified from a fungus, Absidia coerulea, and characterized. The enzyme was purified 516-fold to homogeneity by means of 65-80% ammonium sulfate precipitation followed by chromatography on Butyl Toyoperal-650M, Gigapite (hydroxyapatite), and DEAE Toyopearl-650M.
X D, Gao, T, Katsumoto, K, Onodera
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International Journal of Biological Macromolecules, 2020
The chitin deacetylase produced by marine strain Nitratireductor aquimarinus MCDA3-3 (NaCDA) was purified by using ammonium sulfate precipitation, Q Sepharose, and Superdex column chromatography. The purified NaCDA showed 75-fold purity, 50 U/mg specific activity with 28.5% yield. The purified CDA molecular weight was about 36 kDa.
Shujun Wang, Yaowei Fang
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The chitin deacetylase produced by marine strain Nitratireductor aquimarinus MCDA3-3 (NaCDA) was purified by using ammonium sulfate precipitation, Q Sepharose, and Superdex column chromatography. The purified NaCDA showed 75-fold purity, 50 U/mg specific activity with 28.5% yield. The purified CDA molecular weight was about 36 kDa.
Shujun Wang, Yaowei Fang
exaly +3 more sources
Chitin deacetylase 2 is essential for molting and survival of Tetranychus urticae
Pesticide Biochemistry and Physiology, 2021Chitin metabolism has long been considered promising targets for development of biorational pesticides. Considering the increasing challenges of controlling the twospotted spider mite, Tetranychus urticae Koch, the roles of chitin deacetylases (CDAs) during molting process and mite development are explored.
Lihong, Song +4 more
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Kinetics of chitin deacetylase activation by the ionic liquid [Bmim][Br]
Journal of Biotechnology, 2017Chitin deacetylase is the only known enzyme that can deacetylate the N-acetyl-d-glucosamine units in chitin and chitosan to D-glucosamine. Unfortunately, this enzyme, originally obtained from fungi, usually has low activity. Here, we present that it is possible to enhance the activity of chitin deacetylase using the ionic liquid [Bmim][Br]. An increase
Malgorzata M Jaworska, Andrzej Górak
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Chitin deacetylase from Colletotrichum lindemuthianum
1988Publisher Summary Chitin, a β-1,4-1inked homopolymer of N-acetyl-D-glucosamine, is accompanied in the walls of various fungi by chitosan, a polymer similar to chitin by lacking the N-acetyl groups. Chitosan is most likely synthesized by partial deacetylation of the nascent chain of chitin once the latter is formed by chitin synthase from UDP-GlcNAc ...
Heinrich Kauss, Bärbel Bauch
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Carbohydrate Research, 1997
Chitin oligosaccharides of degree of polymerization 2-4 were deacetylated by purified chitin deacetylase isolated from Colletotrichum lindemuthianum to give their corresponding breakdown products after purification by liquid chromatography. Data from FABMS analyses suggested that N,N',N",N"'-tetraacetylchitotetraose and N,N',N"-triacetylchitotriose ...
K, Tokuyasu +4 more
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Chitin oligosaccharides of degree of polymerization 2-4 were deacetylated by purified chitin deacetylase isolated from Colletotrichum lindemuthianum to give their corresponding breakdown products after purification by liquid chromatography. Data from FABMS analyses suggested that N,N',N",N"'-tetraacetylchitotetraose and N,N',N"-triacetylchitotriose ...
K, Tokuyasu +4 more
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A search of an optimal carrier for immobilization of chitin deacetylase
Cellulose, 2008Chitin deacetylase is an enzyme that can play an important role in enzymatic deacetylation of chitosan to obtain polymers with a lower degree of acetylation. As this enzyme has never been immobilized up to now, efforts were directed towards determining both the most suitable carrier and the best method of covalent attachment to the selected carrier. In
Małgorzata M. Jaworska +2 more
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