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A comprehensive account of fungal chitin deacetylases: Aspects and prospects
International Journal of Biological MacromoleculesChitin deacetylases (CDA, EC 3.5.1.41), found in bacteria, insects and fungi, are the only enzymes in nature that catalyzes the conversion of chitin (polymer of N-acetyl-β-D-glucosamine) to chitosan (polymer of β-D-glucosamine). Presently, the commercial production of chitosan involves using harsh chemicals at high temperatures, causing pollution ...
Namrata, Raghavan, Ejaj K, Pathan
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Cloning and Expression of Two Chitin Deacetylase Genes ofSaccharomyces cerevisiae
Yeast, 1997Chitin deacetylase (EC 3.5.1.41), which hydrolyses the N-acetamido groups of N-acetyl-D-glucosamine residues in chitin, has been demonstrated in crude extracts from sporulating Saccharomyces cerevisiae. Two S. cerevisiae open reading frames (ORFs), identified by the Yeast Genome Project, have protein sequence homology to a chitin deacetylase from Mucor
C, Mishra +6 more
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Detection of chitin deacetylase activity after polyacrylamide gel electrophoresis
Analytical Biochemistry, 1990Mucor racemosus and Rhizopus nigricans were used as sources of chitin deacetylases. Crude protein extracts were subjected to polyacrylamide gel electrophoresis at pH 8.9 (Davis system) or 4.3 (Reisfeld system) under native conditions. After electrophoresis, an overlay gel containing 0.1% (w/v) glycol chitin as substrate was incubated in contact with ...
J, Trudel, A, Asselin
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CHITIN DEACETYLASE AND METHOD FOR PRODUCING THE SAME
2003The invention relates to chitin deacetylase sequences and to proteins coded therefrom, which are suitable for converting biopolymers into glucosamine.
FRAUNHOFER GES FORSCHUNG +8 more
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Journal of Agricultural and Food Chemistry
Cascade conversion of chitin into soluble and functional chitooligosaccharides has gained great attention. However, the biotransformation route is still limited to the low catalytic performances of chitin deacetylases (CDAs) and complicated procedures. In this study, a CDA from Arthrobacter sp.
Liwei Qing +7 more
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Cascade conversion of chitin into soluble and functional chitooligosaccharides has gained great attention. However, the biotransformation route is still limited to the low catalytic performances of chitin deacetylases (CDAs) and complicated procedures. In this study, a CDA from Arthrobacter sp.
Liwei Qing +7 more
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Investigation of the thermal stability of chitin deacetylase from Saccharomyces cerevisiae
International Journal of Biological MacromoleculesChitin deacetylase hydrolyzes N-acetyl-D-glucosamine in chitin and chito-oligosaccharides, showing strong potential for industrial chitosan and chito-oligosaccharide production. Although a heat-resistant chitin deacetylase from Saccharomyces cerevisiae (ScCDA2) has been previously reported, the molecular basis of its thermostability remains unclear ...
Hongli, Wei +6 more
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Screening and identification of novel chitin deacetylases for the sustainable valorisation of chitin
Chitin is one of the most abundant organic molecules after lignocellulose, yearly produced in nature in the range of 1010-1011 tons. However, its direct applications are mainly restricted to low value-added products (e.g., animal feed and agricultural fertilizers) because of the high crystallinity and inflexibility of its structure.Melissa Bisaccia +6 more
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