Results 31 to 40 of about 1,561 (158)

Reclamation of Marine Chitinous Materials for Chitosanase Production via Microbial Conversion by Paenibacillus macerans

Marine Drugs, 2018
Chitinous materials from marine byproducts elicit great interest among biotechnologists for their potential biomedical or agricultural applications. In this study, four kinds of marine chitinous materials (squid pens, shrimp heads, demineralized shrimp ...
Chien Thang Doan, Thi Ngoc Tran, Van Bon Nguyen, Anh Dzung Nguyen, San-Lang Wang   +4 more
doaj   +1 more source

Expression and Surface Display of an Acidic Cold-Active Chitosanase in Pichia pastoris Using Multi-Copy Expression and High-Density Cultivation

Molecules, 2022
Chitosanase hydrolyzes β-(1,4)-linked glycosidic bonds are used in chitosan chains to release oligosaccharide mixtures. Here, we cloned and expressed a cold-adapted chitosanase (CDA, Genbank: MW094131) using multi-copy expression plasmids (CDA1/2/3/4) in
Yanhong Peng, Yaping Wang, Xiaoyan Liu, Ronghua Zhou, Xianqing Liao, Yong Min, Lixin Ma, Ying Wang, Ben Rao   +8 more
doaj   +1 more source

Screening for Aspergillus fumigatus strain-2T-2 with high chitosanase production activity and its application in chitosan degradation

Electronic Journal of Biotechnology
Background: High chitosanase-producing microorganisms from natural sources have extensive applications in food and agriculture. This study aimed to optimal conditions for high-activity chitosanase production.
Haoyue Yang, Linsong Wang, Chaojie Xu, Wentong Hao, Ronge Xing, Song Liu, Huahua Yu, Pengcheng Li   +7 more
doaj   +1 more source

Activity Measurement of Chitosanase by an Amperometric Biosensor

Analytical Sciences, 2009
An amperometric biosensor for the detection of glucosamine (GlcN) and chitosan oligosaccharides ((GlcN)n) was introduced for an activity measurement of chitosanase. By using the biosensor, an increase in the anodic current due to the production of GlcN and (GlcN)n by chitosanase was measured in a chitosan solution. The maximum value of the slope of the
Hirosuke, Tatsumi, Hajime, Katano, Tokuji, Ikeda, Hisashi, Kimoto   +3 more
openaire   +3 more sources

Screening, Identification and Conditions Optimization of High Chitosanase Producing Strains, and Analysis of Its Enzymatic Properties

Shipin gongye ke-ji
To screen and identify high chitosanase producing strains and to optimize the fermentation condition and analyze the enzymatic properties of the target strain.
Ying LIANG, Rui LIU, Chaodong SONG, Junjie XIE, Doudou YIN, Xia XU, Dengfeng YANG, Hongyan ZHANG, Naikun SHEN   +8 more
doaj   +1 more source

Controllable preparation of chitosan oligosaccharides via a recombinant chitosanase from marine Streptomyces lydicus S1 and its potential application on preservation of pre-packaged tofu

Frontiers in Microbiology, 2022
Chitosan oligosaccharides (COSs) are widely applied in many areas due to its various biological activities. Controllable preparation of COSs with desired degree of polymerization (DP) via suitable chitosanase is of great value.
Hao Chen, Hao Chen, Hao Chen, Hao Chen, Bilian Lin, Bilian Lin, Rui Zhang, Rui Zhang, Zhouliang Gong, Zhouliang Gong, Ming Wen, Ming Wen, Weiming Su, Weiming Su, Jinsong Zhou, Liangzhong Zhao, Liangzhong Zhao, Jianrong Wang, Jianrong Wang, Jianrong Wang   +19 more
doaj   +1 more source

Virtual Screening of Inhibitors for Chitosanases EAG1

Advances in Enzyme Research, 2020
Chitosanases EAG1 is a classical glycoside hydrolase from Bacillus ehimensis. The previous researches showed that this Chitosanases can not only hydrolyze the b1,4-glycosidic bonds of chitosan to COS in different sizes but also keep a high catalytic activity in organic, which was useful for producing chitooligosaccharides and GlcN for use in the food ...
Xiangyang Xu, Zaiwei Song, Yunchao Yin, Faguo Zhong, Junying Song, Jiachao Huang, Wangli Ye, Peng Wang   +7 more
openaire   +2 more sources

Reaction mechanism of chitosanase from Streptomyces sp. N174 [PDF]

Biochemical Journal, 1995
Chitosanase was produced by the strain of Streptomyces lividans TK24 bearing the csn gene from Streptomyces sp. N174, and purified by S-Sepharose and Bio-Gel A column chromatography. Partially (25-35%) N-acetylated chitosan was digested by the purified chitosanase, and structures of the products were analysed by NMR spectroscopy.
T, Fukamizo, Y, Honda, S, Goto, I, Boucher, R, Brzezinski   +4 more
openaire   +2 more sources

Design and Synthesis of a Chitodisaccharide-Based Affinity Resin for Chitosanases Purification

Marine Drugs, 2019
Chitooligosaccharides (CHOS) have gained increasing attention because of their important biological activities. Enhancing the efficiency of CHOS production essentially requires screening of novel chitosanase with unique characteristics.
Shangyong Li, Linna Wang, Xuehong Chen, Mi Sun, Yantao Han   +4 more
doaj   +1 more source

ARTP Mutagenesis Improves the Production of Chitosanase in Bacillus cereus

Shipin gongye ke-ji, 2022
Atmospheric and room temperature plasma(ARTP) Bacillus cereus was used to screen the strains with increased enzyme activity. The results showed that culturing Bacillus cereus in the seed solution for 6 hours could reach the middle and late logarithmic ...
Chaozheng ZHANG, Yi LI, Hua ZHAO
doaj   +1 more source