Context and Mutation in Gymnosperm Chloroplast DNA. [PDF]
Mutations and subsequent repair processes are known to be strongly context-dependent in the flowering-plant chloroplast genome. At least six flanking bases, three on each side, can have an influence on the relative rates of different types of mutation at any given site.
Morton BR.
europepmc +3 more sources
Chloroplast DNA methylation in the kelp Saccharina latissima is determined by origin and possibly influenced by cultivation [PDF]
DNA cytosine methylation is an important epigenetic mechanism in genomic DNA. In most land plants, it is absent in the chloroplast DNA. We detected methylation in the chloroplast DNA of the kelp Saccharina latissima, a non‐model macroalgal species of ...
Lydia Scheschonk +3 more
doaj +2 more sources
An Improved Chloroplast DNA Extraction Procedure for Whole Plastid Genome Sequencing
Background Chloroplast genomes supply valuable genetic information for evolutionary and functional studies in plants. The past five years have witnessed a dramatic increase in the number of completely sequenced chloroplast genomes with the application of
Li-Zhi Gao
exaly +2 more sources
Use of the DNA-specific fluorochrome 4'6-diamidino-2-phenylindole (DAPI) makes it possible to examine in situ the structure of chloroplast DNA (chDNA) with the fluorescence microscope.
Annette W. Coleman
exaly +2 more sources
IN VIVO SPECIFIC LABELING OF CHLAMYDOMONAS CHLOROPLAST DNA
When Chlamydomonas reinhardi is supplied with (methyl-3H)-thymidine, radioactivity is incorporated specifically into chloroplast DNA Chromatographic analysis of the products of enzymatic hydrolysis of the DNA reveals that only thymidine monophosphate has
D. Swinton, P. Hanawalt
exaly +2 more sources
Changes in the relative copy numbers of chloroplast and mitochondrial DNA in the leaves of Vitis vinifera L. after high-temperature treatment in vitro [PDF]
In the context of global warming, studying the consequences of increased temperature on agricultural crops becomes important for predicting the shortand long-term impacts on productivity.
Vodolazhsky Dmitry I., Kryukov Lavr A.
doaj +1 more source
An optimized chloroplast DNA extraction protocol for grasses (Poaceae) proves suitable for whole plastid genome sequencing and SNP detection. [PDF]
BACKGROUND: Obtaining chloroplast genome sequences is important to increase the knowledge about the fundamental biology of plastids, to understand evolutionary and ecological processes in the evolution of plants, to develop biotechnological applications (
Kerstin Diekmann +3 more
doaj +1 more source
SYNTHESIS OF CHLOROPLAST DNA IN ISOLATED CHLOROPLASTS [PDF]
Chloroplasts isolated from Euglena gracilis incorporated both tritiated thymidine 5'-triphosphate and tritiated deoxyadenosine 5'-triphosphate into an acid-stable fraction. The incorporation was dependent on the presence of all four deoxynucleoside triphosphates and was sensitive to treatment with deoxyribonuclease and actinomycin D.
N S, Scott, V C, Shah, R M, Smillie
openaire +2 more sources
Each plant cell has hundreds of copies of the chloroplast genome and chloroplast transgenes do not undergo silencing. Therefore, chloroplast transformation has many powerful potential agricultural and industrial applications.
Kazusato Oikawa +6 more
doaj +1 more source
Phylogenic study of Lemnoideae (duckweeds) through complete chloroplast genomes for eight accessions [PDF]
Background Phylogenetic relationship within different genera of Lemnoideae, a kind of small aquatic monocotyledonous plants, was not well resolved, using either morphological characters or traditional markers.
Yanqiang Ding +6 more
doaj +2 more sources

