Results 271 to 280 of about 2,151,232 (305)
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The characteristics of spinach chloroplast DNA polymerase
Archives of Biochemistry and Biophysics, 1969Purified chloroplast preparations from spinach incorporate [3H]-dTTP1 into an acid-insoluble product. The incorporation reaction is highly dependent on all four deoxynucleoside triphosphates, Mg2+ and a sulphydryl reagent, and is further stimulated by KCl.
Donald Spencer, Paul R. Whitfeld
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A Chloroplast DNA Study of the Agavaceae
Systematic Botany, 1995A chloroplast DNA restriction site analysis of 40 taxa in the Agavaceae s.l. was undertaken to test the accuracy of several current systems of classification. In Cronquist's system, the Agavaceae contain 18 genera of fibrous-leaved, more or less woody plants such as Yucca, Hes- peraloe, Agave, Manfreda, Polianthes, Dracaena, Sansevieria, Nolina ...
David J. Bogler, Beryl B. Simpson
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Miniprep for Chloroplast DNA Isolation
Microchemical Journal, 1993Abstract This paper describes a procedure for the isolation and purification of chloroplastic DNA (cpDNA) designed for undergraduate research projects. It is a modification of the procedures from Doyle and Doyle ( Phytochem. Bull. , 1987, 19 , 11-15), Marmur ( Mol. Biol.
E.P. Oharamays, J.C. Capwell
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Chloroplast DNA from three archegoniates
Planta, 19801. DNA from female and male Sphaerocarpos donnellii (liverwort) plants exhibits at least two species with buoyant densities of 1.703 (main band) and 1.691 (satellite) g cm(-3) in CsCl equilibrium gradients. At least part, if not all, of the satellite DNA is localized in plastids.
Reinhold G. Herrmann +2 more
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Mapping the Chloroplast DNA of Vicia faba
Current Genetics, 1983A complete clone bank representing the chloroplast DNA from Vicia faba has been constructed. A total of 15 fragments (10 Pst1, 1 Pst1-EcoR1 and 4 Sal1 fragments) were inserted into the vector pBR322 and transformed into the E. coli strain HB101. The cloned fragments were used as the main tools in constructing the physical map of Vicia faba for the ...
Kenton Ko +2 more
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Chloroplast DNA from broad bean
Biochimica et Biophysica Acta (BBA) - Nucleic Acids and Protein Synthesis, 1969Abstract Chloroplasts purified by centrifugation through the glycerol density gradients of James and Das 19 followed by the flotation technique of Jagendorf 20 were free from nuclear contamination. DNA was isolated from pure chloroplasts and nuclei of broad bean.
John P. Williams, S.D. Kung
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Chloroplast DNA in Expanding Spinach Leaves
, 1980The proportion of chloroplast DNA in total DNA from spinach leaves has been measured using the second order reassociation kinetics of a 3H-labelled chloroplast DNA probe in total DNA extracts. There was no significant difference between the proportion of
N. Scott, J. Possingham
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Universal primers for amplification of three non-coding regions of chloroplast DNA
Plant Molecular Biology, 1991P. Taberlet +3 more
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