Results 231 to 240 of about 644,201 (301)

Transient Transfection of Rolling‐Circle Amplified DNA in Biomanufacturing‐Relevant Mammalian Cell Lines: A Comparison of Transfection Conditions for Optimal Protein Expression

Biotechnology and Applied Biochemistry, EarlyView.
ABSTRACT Rolling‐circle amplification (RCA) is a completely synthetic and rapid mechanism for scaling‐up bulk DNA that eliminates requirements for large‐scale bacterial fermentation. Several reports have described the surprising ability of large unprocessed (hyperbranched) rolling circle‐amplified DNA (RCA DNA) to effectively transfect cultured cells ...
Evelina Loghin, Erik Kvam, Lisa Lowery, Nathan Hudson, Jenny Keen, Brian Davis, Roger B. Dodd, Christine Surrette, John Nelson   +8 more
wiley   +1 more source

Exploiting non-permissive CHO cells as a rapid and efficient method for recombinant HSV-1 isolation. [PDF]

AMB Express
Kelishadi M, Shahsavarani H, Tabarraei A, Shokrgozar MA, Rahimi A, Teimoori-Toolabi L, Azadmanesh K.   +6 more
europepmc   +1 more source

Na5FeS4 as High‐Capacity Positive Electrode Active Material for All‐Solid‐State Sodium Batteries

Batteries &Supercaps, EarlyView.
All‐solid‐state sodium cells using Na5FeS4, synthesize through a simple and scalable process using sodium polysulfides, as a positive electrode active material exhibit a high reversible capacity of 480 mAh g−¹, which is attributed to the sufficiently high ionic conductivity of the active material and the maintenance of the active material/solid ...
Yuta Doi, Tomoya Otono, Yushi Fujita, Raku Kato, Masato Torii, Jiong Ding, Shigeo Mori, Kota Motohashi, Atsushi Sakuda, Akitoshi Hayashi   +9 more
wiley   +1 more source

Stable overexpression of native and artificial miRNAs for the production of differentially fucosylated antibodies in CHO cells. [PDF]

Eng Life Sci
Schlossbauer P, Naumann L, Klingler F, Burkhart M, Handrick R, Korff K, Neusüß C, Otte K, Hesse F.   +8 more
europepmc   +1 more source

Efficacy, safety and cost‐effectiveness of CAR‐T therapy

British Journal of Clinical Pharmacology, EarlyView.
CAR T‐cells demonstrate high efficacy in blood cancers, including ALL, MM and DLBCL. Innovations target solid tumours despite challenges such as antigen escape. Combination therapies enhance the delivery and infiltration of CAR T cells. Toxicity, cost and resistance remain major barriers to clinical use.
Emina Karahmet Sher, Amina Džidić‐Krivić, Emma Pinjic, Nejra Selak, Kanita Omerbasic, Alexandar Chupin, Andrej Belancic, Almir Fajkic   +7 more
wiley   +1 more source

Increased stable integration efficiency in CHO cells through enhanced nuclear localization of Bxb1 serine integrase. [PDF]

BMC Biotechnol
Huhtinen O, Prince S, Lamminmäki U, Salbo R, Kulmala A.   +4 more
europepmc   +1 more source

Quantitative Analysis Reveals Hitchhiking Drives Polysorbate Hydrolase Persistence Via Host Cell Protein–Antibody Interactions

Biotechnology and Bioengineering, EarlyView.
ABSTRACT Polysorbate‐degrading host cell proteins (HCPs) represent a critical challenge in the manufacturing of monoclonal antibody therapeutics due to their potential to persist during downstream processing. While their enzymatic activity has been characterized, the role of direct HCP‐mAb interactions, particularly those involving polysorbate ...
Melanie Maier, Lukas Griesinger, Matthias Franzreb, Simon Kluters   +3 more
wiley   +1 more source

Effects of autophagy-inhibiting chemicals on sialylation of Fc-fusion glycoprotein in recombinant CHO cells. [PDF]

Appl Microbiol Biotechnol
Lee HM, Park JH, Kim TH, Kim HS, Kim DE, Lee MK, You J, Lee GM, Kim YG.   +8 more
europepmc   +1 more source

Multimodal Chromatography in the Downstream Processing of mAb‐Based Products: Mechanisms, Strategies, and Applications

Biotechnology and Bioengineering, EarlyView.
ABSTRACT Multimodal chromatography has emerged as a powerful tool for the purification of monoclonal antibodies (mAbs) and their derivatives—including antibody fragments (Fabs), Fc‐fusions, bispecific (BsAb), and antibody–drug conjugates (ADCs)—offering enhanced selectivity through the integration of ionic, hydrophobic, hydrogen‐bonding, and π–π ...
Amin Javidanbardan, Marco P․ C․ Marques, Ana M. Azevedo, Daniel G. Bracewell, Stephen Goldrick   +4 more
wiley   +1 more source

A complete workflow for single cell mtDNAseq in CHO cells, from cell culture to bioinformatic analysis. [PDF]

Front Bioeng Biotechnol
Foley A, Lao N, Clarke C, Barron N.
europepmc   +1 more source