Results 281 to 290 of about 188,771 (295)

A CHO cell line resistant to deoxycholic acid

Cancer Letters, 1987
Spontaneous mutants resistant to deoxycholic acid (DCA) have been selected from a CHO cell line AuxBl. One of the colonies or 'lines' selected was subsequently mutagenized by ethylmethanesulfonate (250 micrograms/ml) and a more resistant cell, named alpha 3, has been selected. When AuxBl and alpha 3 were exposed to graded concentrations of DCA for 1 h,
CADERNI, GIOVANNA, Stuart E, Anderson AM, Bruce WR   +3 more
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Disruption of cytoskeleton by methylmercury in cultured CHO cells

Toxicology in Vitro, 1992
The effect of methylmercury (MM) on three main cytoskeletal components [i.e. microtubules (MT), microfilaments (MF) and intermediate filaments (IF)] and on specific biochemical parameters (i.e. glutathione transferase (GST), glutathione reductase (RED), glutathione peroxidase (GSH-Px), glyoxalase 1 (GLY 1) and total -SH groups (TSH) of the cytosolic ...
Vignani, R., Milanesi, C., Di Simplicio, P.   +2 more
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Effects of thialysine on CHO cells growth.

Microbiologica, 1985
Thialysine, the lysine isolog with the 4-methylene group substituted by a sulfur atom, inhibits the growth rate and plating efficiency of Chinese Hamster Ovary (CHO) cells. The inhibition can be reversed by lysine, when added to the culture medium together with thialysine or shortly after; to have a complete reversion a lysine concentration five times ...
M. D. Girolamo, A. D. Girolamo, COCCIA, Raffaella, C. Foppoli, BLARZINO, Carla   +4 more
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Regulated autocrine growth of CHO cells.

Cytotechnology, 2000
The goal of this work was to engineer a CHO cell line capable ofautocrine growth in a fully defined protein-free medium. Thiswas accomplished by stable integration of the genes encodinginsulin-like growth factor I (IGF-I) and transferrin into thegenome of a CHO-K1 cell line. Thelac operator/repressorsystem was used to regulate the expression of the IGF-
Merilyn Sleigh, Masood Baig, Noelle-Anne Sunstrom, Sybille Marie Hunt, Sugiyono, Charles G. Bailey, Peter P. Gray   +6 more
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The Genetic Consequences of the Thy- Mutation to CHO Cells

1985
The behavior and regulation of dNTP pools in somatic cells has long led to speculation that they may play a role in DNA synthesis besides that of simple precursors [2, 20]. While controversy has developed concerning the relationship of dNTP pools to DNA synthesis [18, 19], observations from several laboratories provide convincing evidence that pool ...
Otelinda Gonçalves, Marie Trudel, Mark Meuth   +2 more
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Cultivation of CHO Cells on Novel Microcarriers

2005
CHO cells were successfully cultured on a novel type of microcarriers (ImmobaSil FS) in the bioreactors. Various factors influencing cell attachment, including serum concentration, cell to microcarrier ratio, agitation speed and agitation profile were investigated and the results were compared with those obtained using CultiSpher-G, and glass beads. It
A. Y. Hu, M. Al-Rubeal, Z. Zhang
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A Single‐Cell Model for CHO Cellsa

Annals of the New York Academy of Sciences, 1992
P. Wu, Michael L. Shuler, N. G. Ray
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Glycoengineering of CHO Cells to Improve Product Quality

2017
Chinese hamster ovary (CHO) cells represent the predominant platform in biopharmaceutical industry for the production of recombinant biotherapeutic proteins, especially glycoproteins. These glycoproteins include oligosaccharide or glycan attachments that represent one of the principal components dictating product quality. Especially important are the N-
Michael J. Betenbaugh, Cheng-Yu Chung, Qiong Wang, Bojiao Yin   +3 more
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CULTIVATION OF CHO-CELLS ON MICROCARRIERS DORMACELL

1991
ABSTRACT Four standard types of DORMACELL have been studied concerning their acceptability for the cultivation of CHO-cells. DORMACELL 2.9 proved to be the best carrier type for rapid cell growth whereas during the stationary phase the cells were more firmly attached to DORMACELL 2.0. Additional experiments showed that CHO-cell cultures can easily be
G. Gummich, I. Keller, M. Jacobs, D. Schwengers   +3 more
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CONTINUOUS SUSPENSION CHO CELL CULTURE

1994
ABSTRACT A recombinant DNA CHO cell line which produces t-PA was cultivated continuously in suspension (D = 0.5 h−1). The cultivation consisted of four phases with different ammonium chloride concentrations (0,2.5,5 and 7.5 mM) in the feed medium, causing reactor ammonium levels up to 8 mM.
Henrik Albahn Hansen, Claus Emborg
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