Results 131 to 140 of about 3,708,398 (246)

Automated FRAP microscopy for high‐throughput analysis of protein dynamics in chromatin organization and transcription

open access: yesFEBS Open Bio, EarlyView.
RoboMic is an automated confocal microscopy pipeline for high‐throughput functional imaging in living cells. Demonstrated with fluorescence recovery after photobleaching (FRAP), it integrates AI‐driven nuclear segmentation, ROI selection, bleaching, and analysis.
Selçuk Yavuz   +6 more
wiley   +1 more source

Single‐molecule DNA flow‐stretch assays for high‐throughput DNA–protein interaction studies

open access: yesFEBS Open Bio, EarlyView.
We describe an optimised single‐molecule DNA flow‐stretch assay that visualises DNA–protein interactions in real time. Linear DNA fragments are tethered to a surface and stretched by buffer flow for fluorescence imaging. Using λ and φX174 DNA, this protocol enhances reproducibility and accessibility, providing a versatile approach for studying diverse ...
Ayush Kumar Ganguli   +8 more
wiley   +1 more source

RoundMi: A quantitative method to analyze mitochondrial morphology in mitotic cells

open access: yesFEBS Open Bio, EarlyView.
RoundMi is a workflow for rapid analysis of mitochondrial morphology in mitotic cells. By combining adaptive preprocessing with automated segmentation and quantification, it enables accurate measurements from single focal plane images, reducing acquisition time and computational demands while remaining compatible with high‐throughput fixed and live ...
Elmira Parvindokht Bararpour   +2 more
wiley   +1 more source

Versatile vector tools for efficient protein screening across multiple expression systems

open access: yesFEBS Open Bio, EarlyView.
A unified vector toolkit enables rapid protein expression screening across E. coli, insect, and mammalian cells. A single primer pair amplifies the target gene, which is inserted into any vector via a standardized interface. This streamlined workflow eliminates repeated cloning steps, accelerating the identification of optimal expression conditions for
Zhimin Zhu   +5 more
wiley   +1 more source

Interview with Mary Choice

open access: yes, 2019
Mary Choice (formerly Sullivan) is interviewed by Edward Clark Smith on March 21, 1987 as part of the Western North Carolina Tomorrow Black Oral History Project.
Smith, Edward Clark;
core  

Ruby\u27s Choice, 1989 [PDF]

open access: yes, 1989
1989 Menu from Ruby\u27s Choice restaurant, 116 Free Street. The popular lunch spot was a perennial winner of newspaper readers\u27 polls for best burger in Portland, and was in business on Free Street from late 1985 through 1997.1986 Photographs of Ruby\
Ruby\u27s Choice
core  

Beyond choice architecture: advancing the science of changing behaviour at scale. [PDF]

open access: yesBMC Public Health, 2021
Marteau TM   +3 more
europepmc   +1 more source

UiO‐66 metal–organic frameworks in biomedicine: From structural tunability to bioimaging, photodiagnostics, and photodynamic cancer therapy

open access: yesFEBS Open Bio, EarlyView.
UiO‐66(Zr) metal–organic frameworks are chemically stable, biocompatible, and highly tunable nanomaterials. Their modular structure enables controlled drug delivery, multimodal bioimaging, and light‐activated photodynamic therapy, supporting integrated diagnostic and therapeutic (theranostic) applications in cancer and biomedical research.
Veronika Huntošová   +2 more
wiley   +1 more source

Textiles and Architecture: Enhancing the Urban Fabric By Quilting

open access: yes
Textiles are the architectural material of choice for the temporary and small scale. They are used when architecture does not fully meet a person’s need for shelter, in the form of insulating wall hangings, bedspreads, and clothing.
Smedley, Samuel
core  

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