Results 71 to 80 of about 971,786 (390)

Purification tags markedly affect self‐aggregation of CPEB3

FEBS Letters, EarlyView.
Although recombinant proteins are used to study protein aggregation in vitro, uncleaved tags can interfere with accurate interpretation. Our findings demonstrate that His₆‐GFP and His₁₂ tags significantly affect liquid droplet and amyloid fibril formation in the intrinsically disordered region (IDR) of mouse cytoplasmic polyadenylation element‐binding ...
Harunobu Saito, Yujin Lee, Motoharu Ueno, Naotaka Sekiyama, Masatomo So, Ayako Furukawa, Kenji Sugase   +6 more
wiley   +1 more source

Epithelial cells maintain memory of prior infection with Streptococcus pneumoniae through di-methylation of histone H3

Nature Communications
Epithelial cells are the first point of contact for bacteria entering the respiratory tract. Streptococcus pneumoniae is an obligate human pathobiont of the nasal mucosa, carried asymptomatically but also the cause of severe pneumoniae.
Christine Chevalier, Claudia Chica, Justine Matheau, Adrien Pain, Michael G. Connor, Melanie A. Hamon   +5 more
doaj   +1 more source

Circulating histones as clinical biomarkers in critically ill conditions

FEBS Letters, EarlyView.
Circulating histones are emerging as promising biomarkers in critical illness due to their diagnostic, prognostic, and therapeutic potential. Detection methods such as ELISA and mass spectrometry provide reliable approaches for quantifying histone levels in plasma samples.
José Luis García‐Gimenez, Juan Carlos Ruiz‐Rodríguez, Ricard Ferrer, Raquel Durá, Antonio Artigas, Iván Bajaña, David Bolado López de Andujar, Irene Cánovas‐Cervera, Adrián Ceccato, Luis Chiscano‐Camón, Elena Climent‐Martinez, Georgia García Fernández, Gemma Goma, Verónica Monforte, Beatriz Quevedo‐Sánchez, Adolf Ruiz‐Sanmartín, Antonio Sierra‐Rivera, Nieves Carbonell Monleón   +17 more
wiley   +1 more source

Synaptic, transcriptional, and chromatin genes disrupted in autism

Nature, 2014
The genetic architecture of autism spectrum disorder involves the interplay of common and rare variants and their impact on hundreds of genes. Using exome sequencing, here we show that analysis of rare coding variation in 3,871 autism cases and 9,937 ...
Silvia De Rubeis, Xin He, A. P. Goldberg, Christopher S. Poultney, K. Samocha, A. E. Cicek, Y. Kou, Li Liu, M. Fromer, S. Walker, T. Singh, L. Klei, J. Kosmicki, Shih-Chen Fu, B. Aleksic, M. Biscaldi, P. Bolton, Jessica M. Brownfeld, Jinlu Cai, Nicholas Campbell, Á. Carracedo, Maria H. Chahrour, A. Chiocchetti, H. Coon, Emily L. Crawford, L. Crooks, S. Curran, G. Dawson, E. Duketis, B. Fernandez, L. Gallagher, Evan T. Geller, Stephen J. Guter, R. Hill, I. Ionita-Laza, P. J. González, H. Kilpinen, S. Klauck, A. Kolevzon, Irene O. Lee, Jing Lei, T. Lehtimäki, Chiao-Feng Lin, Avi Ma’ayan, C. Marshall, Alison L. McInnes, B. Neale, M. Owen, N. Ozaki, M. Parellada, J. Parr, S. Purcell, K. Puura, D. Rajagopalan, K. Rehnström, A. Reichenberg, A. Sabo, M. Sachse, Stephan J Sanders, C. Schafer, M. Schulte-Rüther, D. Skuse, C. Stevens, P. Szatmari, K. Tammimies, O. Valladares, A. Voran, Li-San Wang, L. Weiss, A. Willsey, T. Yu, R. Yuen, E. Cook, C. Freitag, M. Gill, C. Hultman, T. Lehner, A. Palotie, G. Schellenberg, P. Sklar, M. State, J. Sutcliffe, C. Walsh, S. Scherer, M. Zwick, J. Barrett, D. Cutler, K. Roeder, B. Devlin, M. Daly, J. Buxbaum   +90 more
semanticscholar   +1 more source

Chromatin association of the SMC5/6 complex is dependent on binding of its NSE3 subunit to DNA [PDF]

, 2015
SMC5/6 is a highly conserved protein complex related to cohesin and condensin, which are the key components of higher-order chromatin structures. The SMC5/6 complex is essential for proliferation in yeast and is involved in replication fork stability and
Adamus, Marek, Alt, Aaron, Jurcisinova, Lenka, Lehmann, Alan R, Liao, Chunyan, Novakova, Marketa, Oliver, Antony W, Palecek, Jan J, Skoupilova, Hana, Vondrova, Lucie, Zabrady, Katerina   +10 more
core   +2 more sources

Insights into pegRNA design from editing of the cardiomyopathy‐associated phospholamban R14del mutation

FEBS Letters, EarlyView.
This study reveals how prime editing guide RNA (pegRNA) secondary structure and reverse transcriptase template length affect prime editing efficiency in correcting the phospholamban R14del cardiomyopathy‐associated mutation. Insights support the design of structurally optimized enhanced pegRNAs for precise gene therapy.
Bing Yao, Qiangbing Yang, Christian J.B. Snijders Blok, Mark A. Daniels, Pieter A. Doevendans, Raymond Schiffelers, Joost P.G. Sluijter, Zhiyong Lei   +7 more
wiley   +1 more source

Statins as anti‐tumor agents: A paradigm for repurposed drugs

Cancer Reports
Background Statins, frequently prescribed medications, work by inhibiting the rate‐limiting enzyme HMG‐CoA reductase (HMGCR) in the mevalonate pathway to reduce cholesterol levels.
Sneha Tripathi, Ekta Gupta, Sanjeev Galande   +2 more
doaj   +1 more source

Base-editing-mediated dissection of a γ-globin cis-regulatory element for the therapeutic reactivation of fetal hemoglobin expression

Nature Communications, 2022
Antoniou and colleagues used base editing to generate a variety of mutations inducing γ-globin and rescue the β-hemoglobinopathy phenotype. This strategy was safe and effective in long-term repopulating hematopoietic stem/progenitor cells.
Panagiotis Antoniou, Giulia Hardouin, Pierre Martinucci, Giacomo Frati, Tristan Felix, Anne Chalumeau, Letizia Fontana, Jeanne Martin, Cecile Masson, Megane Brusson, Giulia Maule, Marion Rosello, Carine Giovannangeli, Vincent Abramowski, Jean-Pierre de Villartay, Jean-Paul Concordet, Filippo Del Bene, Wassim El Nemer, Mario Amendola, Marina Cavazzana, Anna Cereseto, Oriana Romano, Annarita Miccio   +22 more
doaj   +1 more source

Megabase Chromatin Domains Involved in DNA Double-Strand Breaks in Vivo

Journal of Cell Biology, 1999
The loss of chromosomal integrity from DNA double-strand breaks introduced into mammalian cells by ionizing radiation results in the specific phosphorylation of histone H2AX on serine residue 139, yielding a specific modified form named γ-H2AX.
E. Rogakou, C. Boon, C. Redon, W. Bonner
semanticscholar   +1 more source

The Structure of Chromatin [PDF]

Proceedings of the National Academy of Sciences, 1971
The DNA in chromatin of isolated thymus nuclei, and in two different preparations of deoxyribonucleoprotein extracted from chromatin, has been digested with DNase. By variation of the time of reaction and the concentration of enzyme, and by comparison of the action of the enzyme on (protein-free) DNA with its action on DNA complexed with protein in ...
openaire   +3 more sources