Results 111 to 120 of about 137,564 (241)

Optimized lentiviral vector transduction of adherent cells and analysis in sulforhodamine B proliferation and chromatin immunoprecipitation assays. [PDF]

STAR Protoc, 2023
Li L, Trent JC, Eid JE.
europepmc   +1 more source

Integrative Omics Analysis Reveals the Regulation of Hypoxia Tolerance in Large Yellow Croaker (Larimichthys crocea) via the Lipoic Acid Synthase (lias) Gene

Advanced Science, EarlyView.
Lipoic acid synthase (lias) can regulate α‐KG levels through lipoylation, thereby negatively regulating HIF‐1α protein levels via PHD under hypoixa. The Hap2 allele of lias exhibits lower expression levels than Hap1, leading to the accumulation of more HIF‐1α protein and thereby enhancing hypoxia tolerance. ABSTRACT Hypoxia stress seriously affects the
Jie Ding, Songpeng Jia, Ran Meng, Xuelei Wang, Xiongfei Wu, Weiliang Shen, Yibo Zhang, Junquan Zhu   +7 more
wiley   +1 more source

Quantification of protein enrichment at site-specific DNA double-strand breaks by chromatin immunoprecipitation in cultured human cells. [PDF]

STAR Protoc, 2023
Sharma AK, Fitieh AM, Locke AJ, Ali JYH, Ismail IH.   +4 more
europepmc   +1 more source

S100A8/A9‐High Macrophages Activate Intestinal Fibroblasts via mCCL6/hCCL15‐CCR1 Axis to Drive Intestinal Fibrosis in Crohn's Disease

Advanced Science, EarlyView.
S100A8/A9‐high macrophages are markedly enriched in the stenotic intestinal tissue of patients with Crohn's disease. These profibrotic macrophages secrete mCCL6 in a STAT3‐dependent manner. mCCL6 and its human ortholog hCCL15 activate fibroblasts via the CCR1 receptor, thereby driving excessive collagen deposition.
Shu Wang, Jiayun Wang, Junjie Lin, Ziping Ye, Geyujia Zhou, Jingjing Ma, Junjian Sun, Jiang Yu, Yingdi Zhang, Nana Tang, Chunhua Jiao, Xiaojing Zhao, Hongjie Zhang   +12 more
wiley   +1 more source

An efficient chromatin immunoprecipitation (ChIP) protocol for studying histone modifications in peach reproductive tissues. [PDF]

Plant Methods, 2022
Canton M, Farinati S, Forestan C, Joseph J, Bonghi C, Varotto S.   +5 more
europepmc   +1 more source

ANXA2+ Small Extracellular Vesicles Drive Chemoresistance in Anaplastic Thyroid Cancer by Promoting XRCC5 Lactylation and Enhancing Non‐Homologous End‐Joining Repair

Advanced Science, EarlyView.
ANXA2+ sEVs promote cisplatin resistance in ATC by stabilizing the SRC/LDHA interaction, increasing LDHA phosphorylation and activity, and lactate production. Elevated lactate levels promote KAT5‐mediated lactylation of XRCC5 at lysine 265, enhancing its binding to XRCC6.
Shanshan Su, YiShan Xiong, Yuxuan Liang, Xiang Min, Daofeng Dai   +4 more
wiley   +1 more source

Liver Endothelia Orchestrate MASH‐Associated Macrophage Zonation Through FSTL1‐ITGA4 Axis

Advanced Science, EarlyView.
Liver inflammation in MASH fundamentally hinges on the newly defined Kit/STAT2/FSTL1/ITGA4 axis. Declining endothelial Kit collapses normal hepatic zonation, driving an aberrant FSTL1 gradient that recruits pathogenic macrophages. Strikingly, targeting FSTL1‐ITGA4 with anti‐VLA4 restores spatial immune homeostasis and halts disease progression ...
Lin Sun, Zhensheng Yue, Zhiqiang Fang, Hao Xu, Wei Du, Yuwei Ling, Jingjing Liu, Ping Song, Fei He, Juanli Duan, Lin Wang   +10 more
wiley   +1 more source

Psychological Stress Associated Bile Acid Reprogramming Promotes Hepatocellular Carcinoma Progression

Advanced Science, EarlyView.
Depression is increasingly recognized as a risk factor for chronic diseases, yet its biological impact on cancer remains unclear. Using data from more than 490 000 participants across three international cohorts, we show that depression significantly increases the risk of liver cancer.
Ruijiang Zeng, Mengmeng Wang, Kang Wang, Zhuo Xing, Yulong Hong, Tongtong Li, Geng Zong, Chong Yang, Dan Zhang, Zhangling Chen, Xin Jin   +10 more
wiley   +1 more source

A plug and play microfluidic platform for standardized sensitive low-input chromatin immunoprecipitation. [PDF]

Genome Res, 2021
Dirks RAM, Thomas PC, Wu H, Jones RC, Stunnenberg HG, Marks H.   +5 more
europepmc   +1 more source

CXCL10/SLC11A1 Axis Exacerbates Septic Liver Injury by Regulating Neutrophil Extracellular Traps Formation to Drive Macrophage Pro‑Inflammatory Polarization

Advanced Science, EarlyView.
In sepsis‐induced liver injury, marked neutrophil infiltration correlates with disease severity. Neutrophil SLC11A1 drives intracellular Fe2+ and ROS via the Fenton reaction, promoting NETs formation. Hepatocyte‐derived CXCL10 recruits neutrophils and activates the JAK/STAT1/SLC11A1 axis, enhancing NETs‐mediated inflammation.
Haiping Lin, Shicong Zheng, Pei Song, Jie Chang, Zewei Chen, Cang Li, Min Yu, Lude Wang, Shian Yu   +8 more
wiley   +1 more source