Effect of morpholino-mediated knockdown and chromatin immunoprecipitation.
, 2012 (A–I) Effect of morpholino-mediated knockdown of Pax7, Msx1/2, Ets1 and Zic1 on the endogenous expression of FoxD3. Knockdown of Pax7 (A), Msx1/2 (B) and Ets1 (C) results in reduction of endogenous expression of FoxD3 in the cranial neural crest.
Joanne Tan-Cabugao (108591) +4 more
core +1 more source
An optimized chromatin immunoprecipitation protocol using Staph-seq for analyzing genome-wide protein-DNA interactions. [PDF]
STAR Protoc, 2022 Tao F, Rhonda E, He X, Perry JM, Li L.
europepmc +1 more source
Advanced Science, EarlyView.A multifunctional EV‐based nanoplatform (Ang‐TEVs@Gel) was engineered via preconditioning, surface targeting, and ROS‐responsive hydrogel encapsulation to reprogram microglia. This system restored autophagy via miR‐664a‐3p/PIK3CA axis, cleared myelin debris, resolved neuroinflammation, and promoted axon remyelination, ultimately achieving robust motor ...
Wu Xiong +17 more
wiley +1 more source
Optimized lentiviral vector transduction of adherent cells and analysis in sulforhodamine B proliferation and chromatin immunoprecipitation assays. [PDF]
STAR Protoc, 2023 Li L, Trent JC, Eid JE.
europepmc +1 more source
BOULE is Essential for the Dynamic Disassembly of Heat Shock Granules in Male Germ Cells
Advanced Science, EarlyView.BOULE orchestrates stress granule disassembly in germ cells via a two‐pronged mechanism: it promotes G3BP1 ubiquitination by upregulating TRIM27, generating a signal for VCP/FAF2 recruitment, and it maintains G3BP1 and FAF2 protein levels. BOULE deficiency disrupts disassembly complex formation, leading to impaired heat shock granule clearance ...
Xin Li +8 more
wiley +1 more source
An Efficient Chromatin Immunoprecipitation Protocol for the Analysis of Histone Modification Distributions in the Brown Alga Ectocarpus. [PDF]
Methods Protoc, 2022 Bourdareau S +6 more
europepmc +1 more source
Advanced Science, EarlyView.In microglia, STAT3 upregulates TAB2, which promotes NF‐κB activation through its NZF domain‐mediated recognition of K63‐linked ubiquitin chains, leading to inflammatory cytokine release and subsequent neuronal injury. Lumacaftor suppresses TAB2 expression and directly binds the TAB2‐NZF domain to interrupt K63 ubiquitin recognition, thereby blocking ...
Yanhao Zhao +12 more
wiley +1 more source
Microfluidic systems and methods for chromatin immunoprecipitation (ChIP)
, 2014 An integrated microfluidic chromatin immunoprecipitation assay dramatically improves the collection efficiency of ChIP DNA from cells. Immunoprecipitation of chromatin fragments is conducted in a microfluidic chamber with a large fraction of its volume ...
core
Quantification of protein enrichment at site-specific DNA double-strand breaks by chromatin immunoprecipitation in cultured human cells. [PDF]
STAR Protoc, 2023 Sharma AK +4 more
europepmc +1 more source
Advanced Science, EarlyView.The CXCL5/CXCR2 axis directly inhibits ferroptosis in ICC cells by activating the NF‐κB/PTGS2 pathway. Meanwhile, the CXCL5/CXCR2 axis recruits N2 TANs, and the activated PTGS2 promotes the secretion of TNF‐α from the recruited N2 TANs via the CCL2/CCL7/CCR2 pathway, thereby activating the NF‐κB/PTGS2 axis and forming a positive feedback loop, which ...
Chanqi Ye +17 more
wiley +1 more source