Pitfall in the high-throughput quantification of whole blood cyclosporin A using liquid chromatography-tandem mass spectrometry [PDF]
, 2005 In a growing number of laboratories the technique of liquid chromatography-tandem mass spectrometry is used for the quantification of cyclosporin A in whole blood, employing cyclosporin D as the internal standard. Cyclosporin A is extensively metabolized
Spöhrer, Ute, Vogeser, Michael
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Hydrophobic interaction chromatography [PDF]
, 2011 Most proteins and large polypeptides have hydrophobic regions at their surface. These hydrophobic ‘patches’ are due to the presence of the side chains of hydrophobic or non-polar amino acids such as phenylalanine, tryptophan, alanine and methionine ...
Cummins, Phil, O'Connor, Brendan
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A liquid chromatography tandem mass spectrometry method for simultaneous analysis of 46 atmospheric particulate-phase persistent organic pollutants and comparison with gas chromatography/mass spectrometry, [PDF]
, 2017 A novel multi-analyte method for the simultaneous determination of 46 compounds of environmental concern, most of them belonging to the category of persistent organic pollutants, was developed using high-performance liquid chromatography and the results ...
Bartocci, Martina +4 more
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Bioassay-guided isolation and identification of antimicrobial compounds from thyme essential oil by means of overpressured layer chromatography, bioautography and GC-MS [PDF]
, 2012 A simple method is described for efficient isolation of compounds having an antibacterial effect. Two thyme (Thymus vulgaris) essential oils, obtained from the market, were chosen as prospective materials likely to feature several bioactive components ...
Andrea Böszörményi +23 more
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Chromatography of oxysterols [PDF]
Biochimie, 2018 Oxysterols play important roles in development and diseases, but can be highly challenging to analyze. To ensure satisfactory measurements, oxysterols must typically be separated with chromatography prior to detection. Here, we will devote attention to the chromatography of oxysterols, focusing on gas chromatography and liquid chromatography.
Irundika H.K. Dias +2 more
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Purification and analytical characterization of an anti- CD4 monoclonal antibody for human therapy [PDF]
, 1994 A purification process for the monclonal anti-CD4 antibody MAX.16H5 was developed on an analytical scale using (NH&SO, precipitation, anion-exchange chromatography on MonoQ or Q-Sepharose, hydrophobic interaction chromatography on phenyl- Sepharose and ...
Emmrich, F. +8 more
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Self-interaction chromatography as a tool for optimizing conditions for membrane protein crystallization [PDF]
, 2010 The second virial coefficient, or B value, is a measurement of how well a protein interacts with itself in solution. These interactions can lead to protein crystallization or precipitation, depending on their strength, with a narrow range of B values ...
Behlke +26 more
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Continuous preparative thin-layer chromatography [PDF]
, 1967 A short survey of preparative chromatographic methods is given. An apparatus for general use in continuous preparative thin-layer chromatography is described, and its performance compared with some related preparative ...
Visser, K.
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Selection of imprinted nanoparticles by affinity chromatography [PDF]
, 2009 Soluble molecularly imprinted nanoparticles were synthesised via iniferter initiated polymerisation and separated by size via gel permeation chromatography.
Chianella, Iva +4 more
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Gel filtration chromatography [PDF]
, 2011 Gel-filtration chromatography is a popular and versatile technique that permits the effective separation of proteins and other biological molecules in high yield. Here, the basis of the method is described and typical matrix types are contrasted.
Cummins, Phil +2 more
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