Results 1 to 10 of about 519,147 (169)

Chromatography affinity resin with photosynthetically-sourced protein A ligand [PDF]

Scientific Reports
Green, photosynthesizing plants can be proficiently used as cost-effective, single-use, fully biodegradable bioreactors for environmentally-friendly production of a variety of valuable recombinant proteins.
Nisha A. Owens, Pieter H. Anborgh, Igor Kolotilin   +2 more
doaj   +2 more sources

Consecutive Affinity and Ion-Exchange Chromatography for AAV9 Vectors Purification [PDF]

Biomedicines
Background: Irrespective of the rapid development of AAV-based gene therapy, the production of clinical-grade vectors has a bottleneck resulting from product-related impurities such as empty and partially filled capsids, which lack a functional ...
Ozgun Firat Duzenli, George Aslanidi
doaj   +2 more sources

Protein purification with light via a genetically encoded azobenzene side chain [PDF]

Nature Communications
Affinity chromatography is the method of choice for the rapid purification of proteins from cell extracts or culture supernatants. Here, we present the light-responsive Azo-tag, a short peptide comprising p-(phenylazo)-L-phenylalanine (Pap), whose side ...
Peter Mayrhofer, Markus R. Anneser, Kristina Schira, Carina A. Sommer, Ina Theobald, Martin Schlapschy, Stefan Achatz, Arne Skerra   +7 more
doaj   +2 more sources

Affinity and Pseudo-Affinity Membrane Chromatography for Viral Vector and Vaccine Purifications: A Review

Membranes, 2023
Several chromatographic approaches have been established over the last decades for the production of pharmaceutically relevant viruses. Due to the large size of these products compared to other biopharmaceuticals, e.g., proteins, convective flow media ...
Keven Lothert, Michael W. Wolff
doaj   +1 more source

Lectin Purification through Affinity Chromatography Exploiting Macroporous Monolithic Adsorbents

Separations, 2023
Growing medical, engineering, biochemical, and biological interest has led to a steady pace of research and development into polymeric monolithic structures with densely interconnected pores for purifying bio compounds.
Josiane F. da Silva, Clara M. G. Lima, Débora L. da Silva, Ivonea S. do Nascimento, Sarah de O. Rodrigues, Letícia A. Gonçalves, Renata F. Santana, Waseem Khalid, Silvani Verruck, Talha Bin Emran, Irwin R. A. de Menezes, Henrique D. M. Coutinho, Mayeen U. Khandaker, Mohammad R. I. Faruque, Rafael da C. I. Fontan   +14 more
doaj   +1 more source

RNA-Affinity Chromatography

Bio-Protocol, 2013
RNA-affinity chromatography assays are used to identify proteins binding specific RNA sequences. These proteins represent potential factors contributing to the function of RNA molecules. In our lab, we have used this protocol to identify proteins binding
Lucia Morales , Pedro Mateos-Gomez, Luis Enjuanes, Isabel Sola   +3 more
doaj   +1 more source

Kinetic Analysis by Affinity Chromatography

Frontiers in Chemistry, 2019
Important information on chemical processes in living systems can be obtained by the rates at which these biological interactions occur. This review will discuss several techniques based on traditional and high-performance affinity chromatography that ...
Sazia Iftekhar, Susan T. Ovbude, David S. Hage   +2 more
doaj   +1 more source

Selection of imprinted nanoparticles by affinity chromatography [PDF]

, 2009
Soluble molecularly imprinted nanoparticles were synthesised via iniferter initiated polymerisation and separated by size via gel permeation chromatography.
Chianella, Iva, Guerreiro, A., Piletska, Elena V., Piletsky, Sergey A., Whitcombe, M. J.   +4 more
core   +1 more source

Actividad inhibitoria de dihidroxifenilpropenona sobre β-lactamasa de Enterobacter cloacae: estudio preliminar en el desarrollo de fármacos para enfrentar la resistencia bacteriana

Biomédica: revista del Instituto Nacional de Salud, 2014
Introducción. Microorganismos patógenos como Enterobacter cloacae producen enzimas β-lactamasas que les confieren resistencia a los antibioticos β-lactámicos y actualmente se ha identificado la actividad limitada de los inhibidores enzimáticos, de modo ...
Cristina Lucía Mora, Julián Castaño, María Consuelo Jaramillo   +2 more
doaj   +1 more source

Glycoform-resolved FcɣRIIIa affinity chromatography–mass spectrometry

mAbs, 2019
Determination of the impact of individual antibody glycoforms on FcɣRIIIa affinity, and consequently antibody-dependent cell-mediated cytotoxicity (ADCC) previously required high purity glycoengineering.
Steffen Lippold, Simone Nicolardi, Elena Domínguez-Vega, Anna-Katharina Heidenreich, Gestur Vidarsson, Dietmar Reusch, Markus Haberger, Manfred Wuhrer, David Falck   +8 more
doaj   +1 more source