Results 11 to 20 of about 519,147 (169)

Dimerization capacities of FGF2 purified with or without heparin-affinity chromatography. [PDF]

PLoS ONE, 2014
Fibroblast growth factor-2 (FGF2) is a pleiotropic growth factor exhibiting a variety of biological activities. In this article, we studied the capacity of FGF2 purified with or without heparin affinity chromatography to self-associate. Analyzing the NMR
Natalia Platonova, Géraldine Miquel, Liang-Yuan Chiu, Said Taouji, Elisabetta Moroni, Giorgio Colombo, Eric Chevet, Shih-Che Sue, Andreas Bikfalvi   +8 more
doaj   +1 more source

Pharmaceutical And Biomedical Applications Of Affinity Chromatography: Recent Trends And Developments [PDF]

, 2012
Affinity chromatography is a separation technique that has become increasingly important in work with biological samples and pharmaceutical agents. This method is based on the use of a biologically-related agent as a stationary phase to selectively ...
Alexandre, Allenmark, Arata, Bartolini, Basiaga, Bergstrom, Block, Block, Boonjob, Bouriotis, Bourne, Bowers-Komro, Brown, Calvano, Caspi, Cellar, Chaga, Chan, Chattopadhyay, Chen, Chen, Chen, Chen, Chen, Chen, Cheshev, Clarke, Clarke, Cong Bi, Conrad, Cuatrecasas, Dalgaard, David S. Hage, de Frutos M, de Paula, del Castillo, Denizli, Domenici, Dong, Duong-Thi, Efthimia Papastavros, Erika Pfaunmiller, Felix, Friedberg, Garberc-Porekar, Garberc-Porekar, German, Gould, Gustavsson, Gutiérrez, Hage, Hage, Hage, Hage, Hage, Hage, Hage, Hage, Hage, Haginaka, Hattori, Haupt, Hauri, Hermanson, Hjerten, Hodgson, Itakura, Iwaki, Jeanethe A. Anguizola, Jiang, John Vargas, Joseph, Joseph, Joseph, Joseph, Joseph, Jozwiak, Jozwiak, Jozwiak, Jozwiak, Kaliszan, Kalizan, Kim, Kim, Kim, Kitagawa, Kool, Kovarik, Kuester, Kullolli, Labrou, Labrou, Larsson, Lee, Lee, Lewinson, Li, Little, Liu, Liu, Liu, Loun, Loun, Loun, Lowe, Mairal, Mallia, Mallik, Mallik, Mallik, Mallik, Mancini, Matsuda, Mayer, Middle, Moaddel, Moaddel, Monzo, Moser, Moser, Mrabet, Nakamura-Tsuruta, Nakano, Nasal, Nelson, Nelson, Ng, Noctor, Noctor, Nuwaysir, Oates, Ohlson, Ohlson, Ohnmacht, Okanda, Oliveira, Panteghini, Patel, Patel, Pedersen, Peterson, Plavina, Porath, Potyrailo, QIAGEN Group, Qiu, Ren, Romig, Rong Li, Ryan Matsuda, Schiel, Schiel, Schiel, Schiel, Schreimer, Silver, Sing, Singhal, Slon-Usakiewicz, Soman, Stevenson, Strandh, Suksrichavalita, Tachibana, Takeda, Talbert, Thomas, Todorova, Tong, Tong, Tong, Turkova, Tweed, Uzun, Victor, Vivekananda, Vosseller, Walters, Wang, Wang, Wikstroem, Wilchek, Winzor, Wu, Xiwei Zheng, Xu, Xuan, Xuan, Yamamoto, Yang, Yang, Yang, Ye, Yoo, Yoo, Yoo, Yoo, Yoo, Zeng, Zeng, Zhao, Zhao   +201 more
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Purification of Mg2+-dependent phosphatidate phosphohydrolase from rat liver: new steps and aspects [PDF]

, 2005
A new procedure for the partial purification of Mg2+-dependent, N-ethylmaleimide-sensitive phosphatidate phosphohydrolase (Mg2+-PAP; EC 3.1.3.4) from rat liver cytosol is described, using protein precipitation with MgCl2, gel filtration on Sephacryl S ...
Butterwith, Cascales, DeMattos, Hopewell, Hosaka, Martin, Mavis, Minami, Nemoto, Nishida, Pollard   +10 more
core   +1 more source

Matrices and Affinity Ligands for Antibody Purification and Corresponding Applications in Radiotherapy

Biomolecules, 2022
Antibodies have become an important class of biological products in cancer treatments such as radiotherapy. The growing therapeutic applications have driven a demand for high-purity antibodies. Affinity chromatography with a high affinity and specificity
Aiying Xue, Saijun Fan
doaj   +1 more source

Purification of baculovirus vectors using heparin affinity chromatography

Molecular Therapy: Methods & Clinical Development, 2016
Baculoviruses are commonly used for recombinant protein and vaccine production. Baculoviruses are nonpathogenic to vertebrates, have a large packaging capacity, display broad host and cell type tropism, infect both dividing and nondividing cells, and do ...
Md Nasimuzzaman, Danielle Lynn, Johannes CM van der Loo, Punam Malik   +3 more
doaj   +1 more source

Non-targeted characterization of attributes affecting antibody-FcγRIIIa V158 (CD16a) binding via online affinity chromatography-mass spectrometry

mAbs, 2022
Antibodies facilitate targeted cell killing by engaging with immune cells such as natural killer cells through weak binding interactions with Fcγ receptors on the cell surface.
Daniel W. Woodall, Thomas M. Dillon, Kevin Kalenian, Rupa Padaki, Scott Kuhns, David J. Semin, Pavel V. Bondarenko   +6 more
doaj   +1 more source

A three-step purification of human α1-acid glycoprotein [PDF]

, 1984
α1-Acid glycoprotein (AGP) was purified to homogeneity by a 3-step procedure using pseudo-ligand affinity chromatography on immobilized Cibacron blue F3GA, Procion red HE3B, and preparative column isoelectric focusing.
Arnaud, Philippe, Bienvenu, Jacques, Laurent, Philippe, Miribel, Laurent, Vallve, Charles   +4 more
core   +1 more source

A Monomer-to-Trimer Transition of the Human Mitochondrial Transcription Termination Factor (mTERF) Is Associated with a Loss of in Vitro Activity [PDF]

, 2004
The human mitochondrial transcription termination factor (mTERF) is a nuclear-encoded 39-kDa protein that recognizes a mtDNA segment within the mitochondrial tRNALeu(UUR) gene immediately adjacent to and downstream of the 16 S rRNA gene. Binding of mTERF
Asin-Cayuela, Jordi, Attardi, Giuseppe, Helm, Mark   +2 more
core   +1 more source

Identification of novel components of Trypanosoma brucei editosomes [PDF]

, 2003
The editosome is a multiprotein complex that catalyzes the insertion and deletion of uridylates that occurs during RNA editing in trypanosomatids. We report the identification of nine novel editosome proteins in Trypanosoma brucei.
Carmean, Nicole, Ernst, Nancy L, Panigrahi, Aswini K, Salavati, Reza, Schnaufer, Achim, Stuart, Kenneth, Wang, Bingbing   +6 more
core   +2 more sources

Produção, purificação e aumento da performance de ciclodextrina glicosiltransferases para produção de ciclodextrinas Production, purification and performance improve of cyclodextrin glycosyl transferases to cyclodextrins production

Química Nova, 2009
Biospecific affinity chromatography was used to purify three cyclodextrin glycosyl transferases (CGTases) obtained from microorganisms isolated of soil.
Cristiane Moriwaki, Cassiana Mazzer, Rúbia Pazzetto, Graciette Matioli   +3 more
doaj   +1 more source