Glycan-specific whole cell affinity chromatography: A versatile microbial adhesion platform
MethodsX, 2014 We have sought a universal platform for elucidating and exploiting specificity of glycan-mediated adhesion by potentially uncharacterized microorganisms. Several techniques exist to explore microbial interactions with carbohydrate structures.
Maxwell L. Van Tassell +2 more
doaj +1 more source
Synthesis of endohedral iron-fullerenes by ion implantation [PDF]
, 2015 In this paper, we discuss the results of our study of the synthesis of endohedral iron-fullerenes. A low energy Fe+ ion beam was irradiated to C60 thin film by using a deceleration system. Fe+-irradiated C60 thin film was analyzed by high performance liquid chromatography and laser desorption/ionization time-of-flight mass spectrometry. We investigated
arxiv +1 more source
ppiTrim: Constructing non-redundant and up-to-date interactomes [PDF]
, 2011 Robust advances in interactome analysis demand comprehensive, non-redundant and consistently annotated datasets. By non-redundant, we mean that the accounting of evidence for every interaction should be faithful: each independent experimental support is ...
Stojmirović, Aleksandar, Yu, Yi-Kuo
core +3 more sources
Evaluating pre-treatment methods in the isolation and purification of monoclonal IgG antibody [PDF]
, 2008 In this study, the effects of different sample preparation techniques on the separation of monoclonal antibody IgG I were inve st igated experimentally. Monoclonal IgG I was obtained from hybridoma cell line TB /C3 transfected with bcl-2 carrier plasmid.
Abdul Majid, Fadzilah Adibah +1 more
core
پزشکی بالینی ابن سینا, 2005 Western blotting or immunoblotting commonly use for study of reaction between antigens and antibodies. Denaturation of many proteins in immunoblotting can affect greatly the reactivity of antibodies and outcome of the procedure.
Ramin Karimi +4 more
doaj
Rapid determination of serum albumin partition coefficients using affinity chromatography
Environmental Advances, 2022 Sorption to serum albumin is essential for characterizing the distribution of chemicals in organisms. It impacts the concentration of the freely dissolved chemical in vivo and in vitro, and needs to be considered when effect concentrations at the target ...
Nadin Ulrich, Alexander Böhme
doaj
Immobilized metal affinity chromatography co-purifies TGF-β1 with histidine-tagged recombinant extracellular proteins. [PDF]
PLoS ONE, 2012 Extracellular recombinant proteins are commonly produced using HEK293 cells as histidine-tagged proteins facilitating purification by immobilized metal affinity chromatography (IMAC).
Jasvir Kaur, Dieter P Reinhardt
doaj +1 more source
Purification and quantification of recombinant Epstein-Barr viral glycoproteins gp350/220 from Chinese hamster ovary cells [PDF]
, 1992 Truncated Epstein-Barr virus (EBV) membrane antigen gp350/220 (EBV-MA) lacking the membrane anchor was expressed and secreted into the medium of recombinant Chinese hamster ovary cells that had been cultured in Plasmapur hollow-fibre modules using ...
Hessing, M. +4 more
core +1 more source
Isolation and purification of HLA-DR antigen from Daudi cell line by immunoaffinity chromatography
Journal of Basic Research in Medical Sciences, 2017 Introduction: The major histocompatibility complex (MHC) is a group of cell surface proteins that are essential for recognizing foreign molecules in human and other mammals.
Zahra Khayyati, Fatemeh Yari
doaj
Quantification of Captopril using Ultra High Performance Liquid Chromatography [PDF]
arXiv, 2021 We develop an efficient, sensitive, accurate, cost effective, and greener liquid chromatography method for the determination of a quantification method concerning Captopril in pharmaceutical dosage forms by using ultra high performance liquid chromatography (UHPLC) equipment.
arxiv