Results 1 to 10 of about 73,919 (251)
DEAE-Cellulose-Urea Chromatography of Casein in the Presence of 2-Mercaptoethanol
Abstract κ -Casein, after reduction of the disulfide bond(s) by 2-inercaptoethanol, can be separated from α s1 - and β -casein variants, as well as from whole casein, by DEAE-cellulose-urea chromatography. When κ -easein is in the reduced form it elutes from DEAE-cellulose at 0.05m NaCl.
Marvin P. Thompson
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Chromatography of Acidic Polysaccharides on Deae-Cellulose [PDF]
The chromatography of polysaccharides on resinous ion-exchangers has been reported by Steiner, Neukom, and Deuel (1958). Neukom et al. (1960) later reported the chromatography of a variety of wheat and sugar-beet polysaccharides on DEAEcellulose. Only "neutral" polysaccharides could be eluted at pH 6-8 by increasing buffer and salt concentrations; acid
M. Jermyn
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PURIFICATION OF PEROXIDASE-LABELED ANTIBODY BY USING DEAE-CELLULOSE CHROMATOGRAPHY
A method which facilitates the separation of the conjugated γ-globulin from both non-labeled antibody and unreacted peroxidase by use of DEAE-cellulose chromatography was shown in this paper. Using the new method of conjugation reported by Nakane and Kawaoi (1974), the isoelectric point of the peroxidase-labeled antibody was found appreciably to shift ...
S. YAMASHITA, N. YAMAMOTO, K. YASUDA
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Detoxification of pesticide residues requires effective methods. In this regard, the adsorption efficiency of diethylaminoethyl cellulose (DEAE-cellulose) as an adsorbent material for the removal of 2,4-dichlorophenoxyacetic acid (2,4-D) from water at ...
Jagadeesh Kodali +4 more
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Comparative hydrolysis effect of industrial xylanase purified from Bacillus stearothermophilus (ATCC 12980) on two kinds of xylan [PDF]
Xylan the major portion of the hemicellulose of plant cell wall is a heterogeneous polysaccharide. Xylanases(EC:3.2.1.8) are enzymes obtained from different species of microorganisms that degrade the xylosidic linkages of xylan’s backbone producing ...
Zohreh Faezi Zadeh, Amir Gharib
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Properties of Folate Binding Protein Purified from Cow's Milk
Folic acid played an important role in the metabolism of the body. To measure the serum folic acid levels could use the folate binding protein (FBP) from cow's milk with a technique analogous to ELISA.
A SUBANDRATE +2 more
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Acid phosphatase and ribonuclease extracted with 0.1 M sodium acetate buffer, pH 5.1 from Dactylis glomerata seeds, and partially purified by means of 70% ethanol precipitation showed electrophoretic and Chromatographic heterogeneity.
Eleonora Wieczorek +2 more
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This study was aimed to use digestive duct of common carp Cyprinus Carpio L. as economic source for fish lipase extraction . The enzyme was extracted using potassium phosphate buffer (pH 7 , 0.2 M ).
Hind. K. Ali +2 more
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Chitinases from pitcher fluid of Nepenthes distillatoria
Chitinases belong to a diverse family of enzymes among many other hydrolytic enzymes of the pitcher fluid of Nepenthes sp. There are no existing studies on identification of chitinases in Nepenthes distillatoria the only representative of the genus ...
R. W. K. M. Senevirathna +2 more
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Isolation and fatty acid composition of the plant sulfolipid and galactolipids*
The plant sulfolipid has been isolated from Chlorella pyrenoidosa cells and from alfalfa leaves by chromatography on Florisil and DEAE cellulose columns.
John S. O'Brien, A.A. Benson
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