Results 21 to 30 of about 16,885 (184)
The Effect of Temperature on the Chromatography of Insulin on Deae-Cellulose [PDF]
Australian Journal of Biological Sciences, 1960 The effect of ionic strength (range 0,15-0, 3), pH (range 7-9), and temperature (range I-25°C) on the chromatographic behaviour of three samples of insulin on diethylaminoethyl (DEAE)-cellulose columns has been studied. These three factors have a similar effect, a decrease of temperature or pH and an increase in ionic strength lowering the elution ...
IJ O'donnell, EOP Thompson
openaire +1 more source
The Iraqi Journal of Agricultural science, 2022 This study was aimed to use digestive duct of common carp Cyprinus Carpio L. as economic source for fish lipase extraction . The enzyme was extracted using potassium phosphate buffer (pH 7 , 0.2 M ).
Hind. K. Ali +2 more
doaj +1 more source
Molecules, 2017 A 17.5-kDa trypsin inhibitor was purified from Phaseolus vulgaris cv. “gold bean” with an isolation protocol including ion exchange chromatography on DEAE-cellulose (Diethylaminoethyl-cellulose), affinity chromatography on Affi-gel blue gel, ion exchange
Miao Li +5 more
doaj +1 more source
Immunological activities of polysaccharide extracted from Elaeagnus angustifolia L.
CyTA - Journal of Food, 2018 The study was to investigate the immunological activities of polysaccharide isolated from Elaeagnus angustifolia L. The extracted Elaeagnus angustifolia L. polysaccharide (EAP) was purified by DEAE cellulose-52 chromatography (DEAE-52) and Sephadex G-100
Na-xin Sun +7 more
doaj +1 more source
Chromatography of McCaslan Pole Bean Hemagglutinin on DEAE-Cellulose
Experimental Biology and Medicine, 1959 SummaryA crude preparation of McCaslan Pole Bean phytohemagglutinin has been fractionated chromatographically on DEAE-cellulose. Hemagglutinating activity, followed by testing each fraction with an rbc panel, was found in 3 groups of fractions. In each of these groups the activity was panagglutinating and gave the same titers with each rbc used in ...
M D, PRAGER, R J, SPEER
openaire +2 more sources
Purification of rat pancreatic lipase
Journal of Lipid Research, 1968 A procedure for the isolation of lipase (glycerolester hydrolase, EC 3.1.1.3) from rat pancreas is described. The purification scheme includes homogenization of the pancreas. centrifugation at 3,000 rpm, centrifugation at 40,000 rpm.
Lewis I. Gidez
doaj +1 more source
Chromatography of Acidic Polysaccharides on Deae-Cellulose [PDF]
Australian Journal of Biological Sciences, 1962 The chromatography of polysaccharides on resinous ion-exchangers has been reported by Steiner, Neukom, and Deuel (1958). Neukom et al. (1960) later reported the chromatography of a variety of wheat and sugar-beet polysaccharides on DEAEcellulose. Only "neutral" polysaccharides could be eluted at pH 6-8 by increasing buffer and salt concentrations; acid
openaire +1 more source
Purification of Rennet by DEAE-Cellulose Chromatography
Journal of the agricultural chemical society of Japan, 1966 レンネットをDEAE-セルロースカラムに吸着させ, 0.1M燐酸緩衝液(pH 5.7)に0.1, 0.2および0.3M NaClを加えて調製した溶出液および0.1N HClで順次溶出させてレンニンを分離精製し,あわせてレンネット中に混在するペプシンをも分離し,その活性割合を測定した. (1) 0.1M燐酸緩衝液(pH 5.7)に添加するNaClの濃度を0.1Mから0.2Mに増加した溶出液で得られるFraction 3に凝乳力カが存在し, pH 6.62,イオン強度0.2の燐酸緩衝液を用いた電気泳動,沈降分析からみて均一性の高いレンニンが得られた.
YOSHINO, Umeo +3 more
openaire +2 more sources
Shipin gongye ke-ji, 2023 In order to obtain the best extraction process of sulfated polysaccharides from Kappaphycus alvarezii and determine its antiallergic activity, the single factor experiments were carried out firstly.
Tianxiang WU +7 more
doaj +1 more source
Biochemical investigations on endosperm development in Iris pseudoacorus L.
Acta Societatis Botanicorum Poloniae, 2015 In the successive development phases the nitrogen and protein fractions were determined and ion exchanger chromatography of globulins was performed on DEAE cellulose in the endosperm of the chalasal and micropylar poles of Iris pseudoacorus seeds.
L. Konopska
doaj +1 more source