Results 21 to 30 of about 729,330 (210)

The Effect of Temperature on the Chromatography of Insulin on Deae-Cellulose [PDF]

open access: yesAustralian Journal of Biological Sciences, 1960
The effect of ionic strength (range 0,15-0, 3), pH (range 7-9), and temperature (range I-25°C) on the chromatographic behaviour of three samples of insulin on diethylaminoethyl (DEAE)-cellulose columns has been studied. These three factors have a similar effect, a decrease of temperature or pH and an increase in ionic strength lowering the elution ...
IJ O'donnell, EOP Thompson
openaire   +1 more source

Isolation and fatty acid composition of the plant sulfolipid and galactolipids*

open access: yesJournal of Lipid Research, 1964
The plant sulfolipid has been isolated from Chlorella pyrenoidosa cells and from alfalfa leaves by chromatography on Florisil and DEAE cellulose columns.
John S. O'Brien, A.A. Benson
doaj   +1 more source

EXTRACTION, PURIFICATION AND CHARACTERIZATION OF LIPASE FROM THE DIGESTIVE DUCT OF COMMON CARP CYPRINUS CARPIO L.

open access: yesThe Iraqi Journal of Agricultural science, 2022
This study was aimed to use digestive duct of common carp Cyprinus Carpio L. as economic source for fish  lipase extraction . The enzyme was extracted using potassium phosphate buffer (pH 7 , 0.2 M ).
Hind. K. Ali   +2 more
doaj   +1 more source

Chromatography of Acidic Polysaccharides on Deae-Cellulose [PDF]

open access: yesAustralian Journal of Biological Sciences, 1962
The chromatography of polysaccharides on resinous ion-exchangers has been reported by Steiner, Neukom, and Deuel (1958). Neukom et al. (1960) later reported the chromatography of a variety of wheat and sugar-beet polysaccharides on DEAEcellulose. Only "neutral" polysaccharides could be eluted at pH 6-8 by increasing buffer and salt concentrations; acid
openaire   +1 more source

Isolation and Characterization of a Phaseolus vulgaris Trypsin Inhibitor with Antiproliferative Activity on Leukemia and Lymphoma Cells

open access: yesMolecules, 2017
A 17.5-kDa trypsin inhibitor was purified from Phaseolus vulgaris cv. “gold bean” with an isolation protocol including ion exchange chromatography on DEAE-cellulose (Diethylaminoethyl-cellulose), affinity chromatography on Affi-gel blue gel, ion exchange
Miao Li   +5 more
doaj   +1 more source

Milk-Clotting Protease From <i>Bacillus stercoris</i>NCCP-3139: A Potential Microbial Rennet for Cheese Production. [PDF]

open access: yesFood Sci Nutr
A milk‐clotting protease from Bacillus stercoris NCCP‐3139 was isolated from soil and purified via ammonium sulfate precipitation and sequential chromatographic techniques, yielding a homogeneous enzyme confirmed by SDS–PAGE. The protease exhibited high catalytic selectivity, with a marked increase in milk‐clotting‐to‐proteolytic activity ratio (50–550)
Sibtain M   +8 more
europepmc   +2 more sources

Purification of Rennet by DEAE-Cellulose Chromatography

open access: yesJournal of the agricultural chemical society of Japan, 1966
レンネットをDEAE-セルロースカラムに吸着させ, 0.1M燐酸緩衝液(pH 5.7)に0.1, 0.2および0.3M NaClを加えて調製した溶出液および0.1N HClで順次溶出させてレンニンを分離精製し,あわせてレンネット中に混在するペプシンをも分離し,その活性割合を測定した. (1) 0.1M燐酸緩衝液(pH 5.7)に添加するNaClの濃度を0.1Mから0.2Mに増加した溶出液で得られるFraction 3に凝乳力カが存在し, pH 6.62,イオン強度0.2の燐酸緩衝液を用いた電気泳動,沈降分析からみて均一性の高いレンニンが得られた.
YOSHINO, Umeo   +3 more
openaire   +2 more sources

Non-destructive evaluation of historical paper based on pH estimation from VOC emissions [PDF]

open access: yes, 2007
Volatile organic compounds (VOCs) emitted from materials during degradation can be a valuable source of information. In this work, the emissions of furfural and acetic acid from cellulose were studied using solid-phase micro-extraction (SPME) in ...
Kolar, J.   +12 more
core   +1 more source

Immunological activities of polysaccharide extracted from Elaeagnus angustifolia L.

open access: yesCyTA - Journal of Food, 2018
The study was to investigate the immunological activities of polysaccharide isolated from Elaeagnus angustifolia L. The extracted Elaeagnus angustifolia L. polysaccharide (EAP) was purified by DEAE cellulose-52 chromatography (DEAE-52) and Sephadex G-100
Na-xin Sun   +7 more
doaj   +1 more source

Chromatography of McCaslan Pole Bean Hemagglutinin on DEAE-Cellulose

open access: yesExperimental Biology and Medicine, 1959
SummaryA crude preparation of McCaslan Pole Bean phytohemagglutinin has been fractionated chromatographically on DEAE-cellulose. Hemagglutinating activity, followed by testing each fraction with an rbc panel, was found in 3 groups of fractions. In each of these groups the activity was panagglutinating and gave the same titers with each rbc used in ...
M D, PRAGER, R J, SPEER
openaire   +2 more sources

Home - About - Disclaimer - Privacy