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Extraction, Structural Characterization and Biological Activities of Polysaccharides Derived From <i>Hericium erinaceus</i>. [PDF]

open access: yesFood Sci Nutr
This review summarizes the methods used to extract and purify H. erinaceus polysaccharides (HEP), primarily hot water extraction and column chromatography. Structural characterization indicates that the molecular weights of HEP range from 2.1 to 75,000 kDa, with glucose as the dominant monosaccharide and β‐glycosidic bonds, notably β‐glucans with 1 → 3
Sun N, Li C, Liang W, Wu Z, Han X, Xu S.
europepmc   +2 more sources

Historical Insights Into the Purification of Polyphenol Oxidase From Plants. [PDF]

open access: yesFood Sci Nutr
Overview of purification methods for polyphenol oxidase. Advancements in achieving partial or homogeneous purification. Key biochemical properties relevant to polyphenol oxidase. ABSTRACT Over the past 50 years, different methods have been developed to purify polyphenol oxidase (PPO), a key enzyme involved in the oxidation of phenolic compounds in ...
Vazquez-Lima CG   +4 more
europepmc   +2 more sources

Optimization of Ultrasonic Extraction Process and Anti-allergic Activity of Sulfated Polysaccharides from Kappaphycus alvarezii

open access: yesShipin gongye ke-ji, 2023
In order to obtain the best extraction process of sulfated polysaccharides from Kappaphycus alvarezii and determine its antiallergic activity, the single factor experiments were carried out firstly.
Tianxiang WU   +7 more
doaj   +1 more source

Purification of rat pancreatic lipase

open access: yesJournal of Lipid Research, 1968
A procedure for the isolation of lipase (glycerolester hydrolase, EC 3.1.1.3) from rat pancreas is described. The purification scheme includes homogenization of the pancreas. centrifugation at 3,000 rpm, centrifugation at 40,000 rpm.
Lewis I. Gidez
doaj   +1 more source

Mitochondrial protein import [PDF]

open access: yes, 1988
Transport of nuclear-encoded precursor proteins into mitochondria includes proteolytic cleavage of aminoterminal targeting sequences in the mitochondrial matrix. We have isolated the processing activity from Neurospora crassa.
Hartl, Franz-Ulrich   +5 more
core   +1 more source

Purification of Rennet by DEAE-Cellulose Chromatography

open access: yesJournal of the agricultural chemical society of Japan, 1966
レンネットをDEAE-セルロースカラムに吸着させ, 0.1M燐酸緩衝液(pH 5.7)に0.1, 0.2および0.3M NaClを加えて調製した溶出液および0.1N HClで順次溶出させてレンニンを分離精製し,あわせてレンネット中に混在するペプシンをも分離し,その活性割合を測定した. (1) 0.1M燐酸緩衝液(pH 5.7)に添加するNaClの濃度を0.1Mから0.2Mに増加した溶出液で得られるFraction 3に凝乳力カが存在し, pH 6.62,イオン強度0.2の燐酸緩衝液を用いた電気泳動,沈降分析からみて均一性の高いレンニンが得られた.
Umeo YOSHINO   +3 more
openaire   +2 more sources

Biochemical investigations on endosperm development in Iris pseudoacorus L.

open access: yesActa Societatis Botanicorum Poloniae, 2015
In the successive development phases the nitrogen and protein fractions were determined and ion exchanger chromatography of globulins was performed on DEAE cellulose in the endosperm of the chalasal and micropylar poles of Iris pseudoacorus seeds.
L. Konopska
doaj   +1 more source

Chromatography of Acidic Polysaccharides on Deae-Cellulose [PDF]

open access: yesAustralian Journal of Biological Sciences, 1962
The chromatography of polysaccharides on resinous ion-exchangers has been reported by Steiner, Neukom, and Deuel (1958). Neukom et al. (1960) later reported the chromatography of a variety of wheat and sugar-beet polysaccharides on DEAEcellulose. Only "neutral" polysaccharides could be eluted at pH 6-8 by increasing buffer and salt concentrations; acid
openaire   +1 more source

The influence of gibberellin on the level of soluble protein and activities of some hydrolases in corn seedlings

open access: yesActa Societatis Botanicorum Poloniae, 2015
The effect of gibberellin on the qualitative and quantitative composition of soluble proteins and the activity of Mg++ dependent and Na+-K+ -stimulated ATPase, RNase and peptidase in five days old maize seedlings were investigated.
P. Masłowski, H. Masłowska
doaj   +1 more source

The incorporation of dimethylaminoethanol and dimethylaminoisopropyl alcohol into Phormia regina phospholipids*

open access: yesJournal of Lipid Research, 1963
The effect of replacing dietary choline with dimethylaminoethanol and dimethylaminoisopropyl alcohol on the larval phospholipids of the blowfly Phormia regina was investigated. It was found that these compounds were incorporated into the phospholipids to
Loran L. Bieber, R.W. Newburgh
doaj   +1 more source

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