Results 11 to 20 of about 73,919 (251)

Extraction, Structural Characterization and Biological Activities of Polysaccharides Derived From <i>Hericium erinaceus</i>. [PDF]

Food Sci Nutr
This review summarizes the methods used to extract and purify H. erinaceus polysaccharides (HEP), primarily hot water extraction and column chromatography. Structural characterization indicates that the molecular weights of HEP range from 2.1 to 75,000 kDa, with glucose as the dominant monosaccharide and β‐glycosidic bonds, notably β‐glucans with 1 → 3
Sun N, Li C, Liang W, Wu Z, Han X, Xu S.
europepmc   +2 more sources

Historical Insights Into the Purification of Polyphenol Oxidase From Plants. [PDF]

Food Sci Nutr
Overview of purification methods for polyphenol oxidase. Advancements in achieving partial or homogeneous purification. Key biochemical properties relevant to polyphenol oxidase. ABSTRACT Over the past 50 years, different methods have been developed to purify polyphenol oxidase (PPO), a key enzyme involved in the oxidation of phenolic compounds in ...
Vazquez-Lima CG, Quintana-Quirino M, Bravo AL, López-Simeon R, Nájera H.   +4 more
europepmc   +2 more sources

Glutamine Phosphoribosylpyrophosphate Amidotransferase-independent Phosphoribosyl Amine Synthesis from Ribose 5-Phosphate and Glutamine or Asparagine [PDF]

, 2007
Phosphoribosylamine (PRA) is the first intermediate in the common pathway to purines and thiamine and is generated in bacteria by glutamine phosphoribosylpyrophosphate (PRPP) amidotransferase (EC 2.4.2.14) from PRPP and glutamine.
Downs, Diana M., Koenigsknecht, Mark J., Ramos, Itzel   +2 more
core   +2 more sources

Isolation and Characterization of a Phaseolus vulgaris Trypsin Inhibitor with Antiproliferative Activity on Leukemia and Lymphoma Cells

Molecules, 2017
A 17.5-kDa trypsin inhibitor was purified from Phaseolus vulgaris cv. “gold bean” with an isolation protocol including ion exchange chromatography on DEAE-cellulose (Diethylaminoethyl-cellulose), affinity chromatography on Affi-gel blue gel, ion exchange
Miao Li, Qin Liu, Yajuan Cui, Dong Li, Hexiang Wang, Tzi Bun Ng   +5 more
doaj   +1 more source

Immunological activities of polysaccharide extracted from Elaeagnus angustifolia L.

CyTA - Journal of Food, 2018
The study was to investigate the immunological activities of polysaccharide isolated from Elaeagnus angustifolia L. The extracted Elaeagnus angustifolia L. polysaccharide (EAP) was purified by DEAE cellulose-52 chromatography (DEAE-52) and Sephadex G-100
Na-xin Sun, Hui-ping Liu, Xu-hui Liu, Yan Zhang, Xiao-qing Liu, Shu Wang, Xiang-xin Xu, Wen-tan Tian   +7 more
doaj   +1 more source

Chromatography of McCaslan Pole Bean Hemagglutinin on DEAE-Cellulose

Experimental Biology and Medicine, 1959
SummaryA crude preparation of McCaslan Pole Bean phytohemagglutinin has been fractionated chromatographically on DEAE-cellulose. Hemagglutinating activity, followed by testing each fraction with an rbc panel, was found in 3 groups of fractions. In each of these groups the activity was panagglutinating and gave the same titers with each rbc used in ...
M D, PRAGER, R J, SPEER
openaire   +2 more sources

Optimization of Ultrasonic Extraction Process and Anti-allergic Activity of Sulfated Polysaccharides from Kappaphycus alvarezii

Shipin gongye ke-ji, 2023
In order to obtain the best extraction process of sulfated polysaccharides from Kappaphycus alvarezii and determine its antiallergic activity, the single factor experiments were carried out firstly.
Tianxiang WU, Zhenxing LI, Yanyan WU, Xianqing YANG, Laihao LI, Shengjun CHEN, Bo QI, Yongqiang ZHAO   +7 more
doaj   +1 more source

Mitochondrial protein import [PDF]

, 1988
Transport of nuclear-encoded precursor proteins into mitochondria includes proteolytic cleavage of aminoterminal targeting sequences in the mitochondrial matrix. We have isolated the processing activity from Neurospora crassa.
Hartl, Franz-Ulrich, Hawlitschek, Gerhard, Neupert, Walter, Schmidt, Bernd, Schneider, Helmut, Tropschug, Maximilian   +5 more
core   +1 more source

Purification of rat pancreatic lipase

Journal of Lipid Research, 1968
A procedure for the isolation of lipase (glycerolester hydrolase, EC 3.1.1.3) from rat pancreas is described. The purification scheme includes homogenization of the pancreas. centrifugation at 3,000 rpm, centrifugation at 40,000 rpm.
Lewis I. Gidez
doaj   +1 more source

Purification of Rennet by DEAE-Cellulose Chromatography

Journal of the agricultural chemical society of Japan, 1966
レンネットをDEAE-セルロースカラムに吸着させ, 0.1M燐酸緩衝液(pH 5.7)に0.1, 0.2および0.3M NaClを加えて調製した溶出液および0.1N HClで順次溶出させてレンニンを分離精製し,あわせてレンネット中に混在するペプシンをも分離し,その活性割合を測定した. (1) 0.1M燐酸緩衝液(pH 5.7)に添加するNaClの濃度を0.1Mから0.2Mに増加した溶出液で得られるFraction 3に凝乳力カが存在し, pH 6.62,イオン強度0.2の燐酸緩衝液を用いた電気泳動,沈降分析からみて均一性の高いレンニンが得られた.
Umeo YOSHINO, Nobuji NAKATANI, Yo TOKORO, Kunio YAMAUCHI   +3 more
openaire   +2 more sources