Results 51 to 60 of about 29,610 (215)

Anti-Platelet Fraction Isolated from Galega Officinalis

open access: yesActa Medica Bulgarica, 2016
A fraction from crude extract of Galega officinalis has been purified by column chromatography on Sephadex G-25, Sepharose 4B, DEAE-Cellulose and Sephadex G-100. The final purification factor of the fraction is 120.
Atanasov A.
doaj   +1 more source

Purification and characterization of a calmodulin-dependent protein kinase that is highly concentrated in brain [PDF]

open access: yes, 1983
A calcium and calmodulin-dependent protein kinase has been purified from rat brain. It was monitored during the purification by its ability to phosphorylate the synaptic vesicle-associated protein, synapsin I.
Bennett, Mark K.   +2 more
core  

The Cell Wall Teichuronic Acid Synthetase (TUAS) Is an Enzyme Complex Located in the Cytoplasmic Membrane of Micrococcus luteus [PDF]

open access: yes, 2010
The cell wall teichuronic acid (TUA) of Micrococcus luteus is a long-chain polysaccharide composed of disaccharide repeating units [-4-β-D-ManNAcAp-(1→6)α-D-Glcp−1-]n, which is covalently anchored to the peptidoglycan on the inner cell wall and extended ...
Alexander, Alice A.   +3 more
core   +4 more sources

Purification of laccase from the fungus Clitopilus prunulus BV18 and its application in bioconversion of tea polyphenols to bioactive theabrownins

open access: yesBiomedical and Biotechnology Research Journal, 2023
Background: Theabrownins (TBs), which are produced from tea leaves, have been shown to have biological. To extract biologically active chemicals from polyphenol structures, an oxidizing enzyme (i.e.
Dinh Giap Vu   +4 more
doaj   +1 more source

Identification of microtubule-associated proteins in the centrosome, spindle, and kinetochore of the early Drosophila embryo. [PDF]

open access: yes, 1989
We have developed affinity chromatography methods for the isolation of microtubule-associated proteins (MAPs) from soluble cytoplasmic extracts and have used them to analyze the cytoskeleton of the early Drosophila embryo.
Alberts, BM, Field, CM, Kellogg, DR
core  

Screening, Purification, and Characterization of Protease Inhibitors From Different Legume Seeds With Cytotoxic and Antimicrobial Effects

open access: yesLegume Science, Volume 7, Issue 4, December 2025.
ABSTRACT This study reports the purification and characterization of protease inhibitors (PIs) from three Egyptian legumes: soybean (Glycine max), cowpea (Vigna unguiculata), and chickpea (Cicer arietinum). Their antimicrobial and cytotoxic activities were also assessed.
Mona M. Ibrahim   +5 more
wiley   +1 more source

Rapid quantitative measurement of lung tissue phospholipids

open access: yesJournal of Lipid Research, 1966
A rapid procedure for the separation of phospholipids of lung tissue into acidic and nonacidic fractions by means of diethylaminoethyl cellulose acetate microcolumns is described.
Louis Gluck   +2 more
doaj   +1 more source

ISOLATION, CHARACTERIZATION AND IMMOBILIZATION OFGLUCOSE OXIDASE FROM ASPERGILLUS NIGER [PDF]

open access: yesMesopotamia Journal of Agriculture, 2007
Two strains of Aspergillus niger were propagated on Czapek broth medium using submerged culture in a shaker incubator at 30 °C and 125 rpm to produce glucose oxidase.
Abdul-Aziz A. Abbas
doaj   +1 more source

Characterization of the mitochondrial processing peptidase of Neurospora crassa [PDF]

open access: yes, 1994
The mitochondrial processing peptidase (MPP) of Neurospora crassa is constituted by an alpha- and a beta-subunit. We have purified alpha-MPP after expression in Escherichia coli while beta-MPP was purified from mitochondria.
Arretz, Michael   +4 more
core  

Effects of TcFLA‐1BP and TcGP72 Deletion on the Infectivity and Survival of Trypanosoma cruzi in Cell Cultures

open access: yesCell Biology International, Volume 49, Issue 11, Page 1395-1410, November 2025.
ABSTRACT Chagas disease, caused by the protozoan Trypanosoma cruzi, is a neglected tropical disease with limited treatment options and no available vaccine. Understanding the role of proteins in the parasite's biological cycle is critical for advancing vaccine development and optimizing therapies.
Normanda Souza‐Melo   +2 more
wiley   +1 more source

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